Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,100,161	C→G	V190L (GTA→CTA)	ansB ←	L‑asparaginase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,100,161	0	C	G	100.0%	174.9 / NA	55	V190L (GTA→CTA)	ansB	L‑asparaginase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (27/28);  total (27/28)

TGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGT  >  NC_000913/3099921‑3100407                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       tGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGgtggtg                                                                                                                                                                                                                                              >  1:136921/1‑251 (MQ=255)        aCCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGttt                                                                                                                                                                                                                                       <  2:446761/251‑1 (MQ=255)           cacaCCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTgg                                                                                                                                                                                                                                    >  2:55833/1‑251 (MQ=255)            acacCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGt                                                                                                                                                                                                                                   >  1:359113/1‑251 (MQ=255)              cccAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGa                                                                                                                                                                                                                                 <  1:313755/250‑1 (MQ=255)              acCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGACGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTAAGCTTAGAGACATCGAATGGCGTGTCGATGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTg                                                                                                                                                                                                                                  >  1:284797/1‑250 (MQ=255)               ccAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGAc                                                                                                                                                                                                                                <  2:387725/251‑1 (MQ=255)                cAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACg                                                                                                                                                                                                                               >  1:134969/1‑251 (MQ=255)                  gcgcTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTc                                                                                                                                                                                                                             <  1:455410/251‑1 (MQ=255)                     cTAACGATGCCATCATAGCCCGCCTCTACCAGTGCTTTAGCCGGAAGATCGGCTGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGCCTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACg                                                                                                                                                                                                                          >  2:151936/1‑251 (MQ=255)                        aCGATGCCATCATAGCCCGCATCTTCCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGTCTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGcc                                                                                                                                                                                                                       <  1:401360/251‑1 (MQ=255)                              ccATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAg                                                                                                                                                                                                                 <  1:406604/251‑1 (MQ=255)                                atcatAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGc                                                                                                                                                                                                                >  1:316335/1‑250 (MQ=255)                                                         ttAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCAAGGCCATCAAGCACGGTGTCATTCATCACTACCCGCAc                                                                                                                                                                                      >  1:348856/1‑251 (MQ=255)                                                              cGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCCTTCAGCTTAGAGACATCGAAAGGCGTGTCGCTGGTCTGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGACGGGCGCTAGGTCGGTGGTGTTGGGTGTGGTGACGTCACGACCATCAAGCCAGGTGTCATTCATCCCTACCAGCACGCCAc                                                                                                                                                                                 >  2:195824/1‑251 (MQ=255)                                                                 aaGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGt                                                                                                                                                                              <  2:13528/251‑1 (MQ=255)                                                                  aGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCAGCAAGCACGGTGTCATTCATCACTACCAGCACGCCTCGGtt                                                                                                                                                                             <  2:265312/251‑1 (MQ=255)                                                                   gATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTg                                                                                                                                                                            >  1:79653/1‑251 (MQ=255)                                                                      cGGATGCGTTAGAGTAGTTAGAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAAGGGCGGGTCGCTGGTAGGCTTACGTGCCGGGGTACGCTGGTAGTCAATCGGACCGTGGTGAATGGAACCCAGAGGACCGTAGTTTACAGACTGGAAGGGCGCTAGGTCGGTGGGGTTGGTTTTGGGGACGTCTCGGCCATCAAGCACGGTGTCATTCAGCACTACTAGGACGGCACGGTTGGc                                                                                                                                                                          <  2:201402/250‑1 (MQ=255)                                                                          ggcggtAGCGTAGTTATAAACAATGCCGACTGTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGGAGTCAATCTGACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGGCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGGCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCTCGGTTTGCGGAgg                                                                                                                                                                     <  2:180744/246‑1 (MQ=255)                                                                                  cGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATc                                                                                                                                                              >  1:175660/1‑250 (MQ=255)                                                                                     aGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGTTTTATCAGCt                                                                                                                                                          <  2:419836/251‑1 (MQ=255)                                                                                           aaaCAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGt                                                                                                                                                     <  1:174560/250‑1 (MQ=255)                                                                                                                gTTCATTCAGCTTAGAGACATCGAATGGCGTGGCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTg                                                                                                                               <  2:183546/251‑1 (MQ=255)                                                                                                                    attcaGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATg                                                                                                                           >  2:320384/1‑251 (MQ=255)                                                                                                                        agcgtAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGAcc                                                                                                                       <  2:136921/247‑1 (MQ=255)                                                                                                                        aGCTTAGAGACATCGAATGGCGTGTCGCTGTTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTATCAGACTTGAAGGTCTCTAGGTCGGTGGTGTTGGTTTGGGTGACGTCACGGCCATCAGGGACGGTGTGATTCATCACGACCAGCACGCCACTGTTGGGGGAGGCTTTGTCATCTGTGGTCACTACCGCGTTATACAGGTTGAATGGAcc                                                                                                                       <  1:451882/251‑1 (MQ=255)                                                                                                                           ttAGAGACATCGACTGGCGTGTCGCTGGCATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCAGTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTc                                                                                                                    >  2:223117/1‑251 (MQ=255)                                                                                                                             agagACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGGTATACAGGTTGAATGGACCGTCt                                                                                                                   <  1:173494/250‑1 (MQ=255)                                                                                                                                 aCATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCt                                                                                                              <  2:6485/251‑1 (MQ=255)                                                                                                                                               gtCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTggacg                                                                                                <  1:14249/251‑1 (MQ=255)                                                                                                                                                       tATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCAtt                                                                                        >  2:356094/1‑251 (MQ=255)                                                                                                                                                        aTGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTg                                                                                       >  1:293250/1‑251 (MQ=255)                                                                                                                                                             tACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGCATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCCCTACCCGCCCGCCACGGTTGGCGGAGGCTTTATCCGCTGCGGTCACTACCGCGTTATCCAGGTTGACTGGACCGTCTGCGCTCATAGACGTGGGCGGACGCTTTgcgc                                                                                    >  2:69401/1‑249 (MQ=255)                                                                                                                                                                gTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGAcc                                                                               >  1:261019/1‑251 (MQ=255)                                                                                                                                                                  gCCGGGGTACTCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCa                                                                              <  2:359113/250‑1 (MQ=255)                                                                                                                                                                    cGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGACGGTGGTGTTGGGTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTGTCAGCGGCGGTCACTACCGCGTTATACAGGTTGCATGGACCGTCTGCGCTCATAGACGGGGACGGACGCATTGCGCCGACCATc                                                                            >  1:347123/1‑250 (MQ=255)                                                                                                                                                                            gCTGGTAGGCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCGCGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGCCCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGt                                                                   <  1:70080/251‑1 (MQ=255)                                                                                                                                                                                   gTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGc                                                            >  2:15174/1‑251 (MQ=255)                                                                                                                                                                                     cAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGACGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACAGCGTTATCCAGGATGAATGGACCGTCTGCGCTCATAGACGTGGCCGGACGCATTGCGCCGCCAATCACCCCCGGTTTGTCGCAt                                                          >  2:224106/1‑251 (MQ=255)                                                                                                                                                                                     cAATCTTACCGTTGGGAATGTAACCCAGAGGACCGTTGTTAACAGACTTGAAGGGCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCTCTACCGCGTTATAAAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACTGACGCATGGCGCCGACCATCACCACCGGTTTGTCGCAt                                                          <  2:55116/251‑1 (MQ=255)                                                                                                                                                                                               gTTGTGAATGTAACCCAGAGGATCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGCCGCATTGCGCCGCCCATCACCACCGGTTTGTCGCACTTCACCGTCa                                                <  2:316335/251‑1 (MQ=255)                                                                                                                                                                                                      aTGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGATGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTTGACGGACGCATGGCGCTGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAg                                         <  1:23209/251‑1 (MQ=255)                                                                                                                                                                                                          aaCCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGaa                                      <  1:55833/250‑1 (MQ=255)                                                                                                                                                                                                          aaCCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGa                                       >  1:450131/1‑249 (MQ=255)                                                                                                                                                                                                             ccAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCCCCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTa                                   >  2:140668/1‑250 (MQ=255)                                                                                                                                                                                                             ccAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTaa                                  <  2:74355/251‑1 (MQ=255)                                                                                                                                                                                                                 agGACCGTAGTTAACAGACTTGACGGCCGCTAGGTCGGTGGTGTTGGTTTCGGTGAAGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCCCGCCCCGGTTGGCGGAGGCTTTATCAGCTGCGGTAACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGCCCATCACCCCCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAga                                >  2:39307/1‑248 (MQ=255)                                                                                                                                                                                                                 agGACCGTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGATGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAg                              <  1:320384/251‑1 (MQ=255)                                                                                                                                                                                                                       gTAGTTAGCAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCGCGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGGCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAAGCGTGGACGTACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTttctt                        <  1:88174/251‑1 (MQ=255)                                                                                                                                                                                                                       gTAGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGCGGAAGTAAGCAGTttctt                        >  2:434784/1‑251 (MQ=255)                                                                                                                                                                                                                         aGTTAACAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTttcttc                       <  2:293250/250‑1 (MQ=255)                                                                                                                                                                                                                             aaCAGACTTGAAGGTCGCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCg                  >  1:135549/1‑251 (MQ=255)                                                                                                                                                                                                                                     tGAAGGTCGATAGGTCGGCGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCAAGGTGTCAATCATCACTACCAGCACGCCACGGATGGCGGAGGCTTTATCAGCTGCGGTCAATACCGCGATATAAAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGCAGGCCGCATTGCGCCGCCCATGACCACGGGTTTGTCGCATTTCAAAGTCAGGTCGCGGAAGTAAGCAGTTTCTTCCAGCGTGTAGGt           >  2:136864/1‑250 (MQ=255)                                                                                                                                                                                                                                             gCTAGGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTgggg  >  2:40538/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       TGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTGGCGGAGGCTTTATCAGCTGCGGTCACTACCGCGTTATACAGGTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGT  >  NC_000913/3099921‑3100407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑