Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,587,112	C→T	A461V (GCA→GTA)	narQ →	sensor histidine kinase NarQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,587,112	0	C	T	95.5%	127.4 / NA	45	A461V (GCA→GTA)	narQ	sensor histidine kinase NarQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (16/26);  minor base G (2/0);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 1.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTG  >  NC_000913/2586866‑2587347                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            acttacGTATCCAGTTGACGTTACTGAAGCGTTCGTTACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGAGTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCTCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGTAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCTTCGTCTGCCTTCTGCCAACCCGGGTACTg                                                                                                                                                                                                                                         <  2:417753/251‑1 (MQ=255)    tacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGa                                                                                                                                                                                                                                       <  1:50767/250‑1 (MQ=255)     acGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGacg                                                                                                                                                                                                                                     <  1:234357/251‑3 (MQ=255)     acGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGAt                                                                                                                                                                                                                                      <  1:322593/250‑1 (MQ=255)      cGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATgc                                                                                                                                                                                                                                    >  1:216095/1‑251 (MQ=255)       gTATCCAGTTGACGTTACTGCAGCGTTCGATACCGGAAGATAACGCCACCGCACACAGTATAATGGCCGCTTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGGTACGTTACAAAATCAAACCAGCGCCCAACTGACCATCGGCTGCCGTCTGCCAACCCTGGTACTGGATgcc                                                                                                                                                                                                                                   >  2:452475/1‑250 (MQ=255)       gTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGAGGCTTATCGGCAGTTACGCGAGCTGGTGACTACTTTCCGCCTGACTCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATgcg                                                                                                                                                                                                                                   <  1:273210/251‑1 (MQ=255)         aTCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCTCGCAAAGTATCATGGCCTATTTTTCCCAGTCTTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGTCGCTTCAGCATTCGGATCTCCCCACCGCATTTATGTATATGTTGGATGCGTTACAAAATCAAGCCAGCGCCTAATTGACCCTCGCCGGCCGCCTGCCATCCCTGGTCCTGTATgcgc                                                                                                                                                                                                                                  <  2:294378/250‑1 (MQ=255)                 gACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGATGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACGCTCGACTGCCGGCTGCCAACCCTGGTACTGGATGCGCAAATGCAgg                                                                                                                                                                                                                         <  1:199095/251‑1 (MQ=255)                  aCGTTACTGAAGCGCTCGATACCGGAAGATAACGCCACCGCCCCAAGTATCATGGCCGCTTTTTCCAAGGCGTTGAATGATGCTTATCGGCAGTTACGCGCGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCAGATCTCCCCTCCGCATTGAGGGACATGCTGGATACGATACCCAATCACACCAGCGCCAAACTGACCCTCAACTGCAGTCTGCCCACCCTGGTACTGGGTGCGCAAATGCaagt                                                                                                                                                                                                                        >  2:188334/1‑248 (MQ=255)                     ttACTGAAGCGTTCGATACAGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTACGCCTGCCGCTGGAGCAGGAGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAAACCAAACAGCGCAAAACTGACACCCGACTGCCGTCTGCAAACCCTGGGACGGGATGCGCAAATGGAgggca                                                                                                                                                                                                                      >  2:265941/1‑247 (MQ=255)                         tGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATttg                                                                                                                                                                                                                 >  1:257661/1‑251 (MQ=255)                             gCGTTCGATACCGGAAGATAACGCCACGGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACGTTCCGCCTGACGCTGCAGCAGGCTGATCTCCCCTCCGCTTTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACGGGCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATGTGTTGc                                                                                                                                                                                                             <  1:158813/251‑1 (MQ=255)                                 tCGATACCGGAAGATAACGCCACCGCACAAAGAATCATGGCCGATTTTTCACAGGCGTTGAATGATGATTATCGGCAGTTACGCGAGCTGTTTACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAAACAGCGCCAAACTGACCCTCGACTGCCGTATGCCAACCATGGTACTGGATGCGCAAATGCAGGAGCAATTGTTGCAAat                                                                                                                                                                                                         >  1:443473/1‑251 (MQ=255)                                     tACCGGACGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTAAGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTAAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAAATGACCCTCGACTGCCGTCTGCCAACCCTGGGACTGGATACGCAAAGGCAGGTGCATTTGTTGCACattttt                                                                                                                                                                                                     >  2:458011/1‑248 (MQ=255)                                      aCCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTc                                                                                                                                                                                                    <  2:99660/251‑1 (MQ=255)                                                     cACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTGCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACGGGAGGCGCAAATGCAGGTGCATGTGTTGCAAATTATTctctacggggtgctga                                                                                                                                                                                     <  2:426467/251‑16 (MQ=255)                                                                     aTGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGCGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGCAGCacgc                                                                                                                                                                     >  1:286379/1‑251 (MQ=255)                                                                                     aGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATc                                                                                                                                                     <  2:411240/251‑1 (MQ=255)                                                                                       gCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGc                                                                                                                                                   >  1:25726/1‑251 (MQ=255)                                                                                                                       gCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGGTGCAAATTAGTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATc                                                                                                                   <  1:452475/251‑1 (MQ=255)                                                                                                                                       cGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCg                                                                                                   <  2:216095/251‑1 (MQ=255)                                                                                                                                            gACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGAt                                                                                               <  1:458011/250‑1 (MQ=255)                                                                                                                                               gCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAACTGCTGGATACGTTACAAAATCAAACCAGCGCCACACTGACCCTCGACTGCCGTCTGCCAACCCTGGAACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCCCCGCGCCGGCCGGCAATCACACGGTTTATAACCGTGATAACg                                                                                           >  1:228440/1‑251 (MQ=255)                                                                                                                                               gCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACg                                                                                           >  1:313637/1‑251 (MQ=255)                                                                                                                                                     gcagGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCacgccaacgcc                                                                                                                                                              <  2:123776/178‑1 (MQ=255)                                                                                                                                                     gcagGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCacgccaacgcc                                                                                                                                                              >  1:123776/1‑178 (MQ=255)                                                                                                                                                                 ccccTCCGCATTGAGGGAAATGCTGGATACGTTTCCAACTCAAACCAGCGCCAAACTGCCCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATgcgc                                                                                                                                                                                                                                  >  1:185862/1‑98 (MQ=255)                                                                                                                                                                 ccccTCCGCATTGAGGGAAATGCTGGATACGGTACAAAATCAAACCAGCGCCAAACTGACCCTCGAGTGCCGTCTGCCAACCCTGGTACTGGATgcgc                                                                                                                                                                                                                                  <  2:185862/98‑1 (MQ=255)                                                                                                                                                                                    aTGCTGGATACGTTACAAAATCAAACCAGCGCCAAACCGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACACCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTc                                                       >  1:138667/1‑250 (MQ=255)                                                                                                                                                                                     tGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTc                                                                                                                                               <  2:230072/161‑1 (MQ=255)                                                                                                                                                                                     tGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTc                                                                                                                                               >  1:230072/1‑161 (MQ=255)                                                                                                                                                                                           aTACGTTACAAAATCAAACTAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAGCGCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAAGGCGATGAAGCACGCCAACGCCAGCTAAATCGCCGGCAGTTGCGTCACCGCGCCGGACGGCAATCATACGGTTTATACCCGGGATAACGGGATTGGTGTCGGTGAACCGAAAGATCCCGAAGGTCATTATGGt                                               <  1:291032/251‑1 (MQ=255)                                                                                                                                                                                                  aCAAAAGCAAACCAGCGCCAAACTGACCCTCGACTGCCGGCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGGTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAata                                        <  1:147829/251‑1 (MQ=255)                                                                                                                                                                                                   aaaaaTCAAACCTGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCGGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGTGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTTAatat                                       <  2:407129/250‑1 (MQ=255)                                                                                                                                                                                                    aaaaTCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATc                                      <  1:304501/251‑1 (MQ=255)                                                                                                                                                                                                     aaaTCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTCCTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATc                                      >  1:29157/1‑250 (MQ=255)                                                                                                                                                                                                             ccAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGa                              >  1:57233/1‑250 (MQ=255)                                                                                                                                                                                                                  gcCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAAcgcgc                        <  2:153849/251‑1 (MQ=255)                                                                                                                                                                                                                      aacggACCCTCGACTGCCGTCTGCCAGCCCTGGTACTGGATGCGCAAATGCAGGTGCAGTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAGCCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGaa                    <  2:224117/247‑1 (MQ=255)                                                                                                                                                                                                                          gACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACg                  <  2:352084/249‑1 (MQ=255)                                                                                                                                                                                                                            cccTCGACTGCCGGCGGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTa              <  2:64226/251‑1 (MQ=255)                                                                                                                                                                                                                               ccGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTAGCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGt           <  2:133731/250‑1 (MQ=255)                                                                                                                                                                                                                                  aCTGCCGTCTGCCAACCCTGGTACTGGATGCGCCAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTAGCGTCACCGCGCCGGGCGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGACTGAATATCTTGCGCGCACTCGCGGAACGGCTAGGTggg        >  2:327056/1‑251 (MQ=255)                                                                                                                                                                                                                                        gTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGTACGCTg  >  2:99678/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            ACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTG  >  NC_000913/2586866‑2587347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑