Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	156,068	A→G	V45A (GTA→GCA)	yadV ←	putative fimbrial chaperone YadV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	156,068	0	A	G	100.0%	59.3 / NA	18	V45A (GTA→GCA)	yadV	putative fimbrial chaperone YadV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/11);  total (7/11)

TTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCA  >  NC_000913/155823‑156277                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                  tttCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGtt                                                                                                                                                                                                               <  2:445649/250‑1 (MQ=255)                                   gTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTTCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGCTCGCTTTTATATATTAcgcg                                                                                                                                                                              >  2:319132/1‑249 (MQ=255)                                            gAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGaaa                                                                                                                                                                   <  2:341921/251‑1 (MQ=255)                                                                          ggTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTaa                                                                                                                                     <  1:188344/251‑1 (MQ=255)                                                                              ctggctgTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCACTAGACGATGAACTAAAAGc                                                                                                                                 >  1:8358/1‑251 (MQ=255)                                                                                                        ttGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCt                                                                                                        <  1:435723/250‑1 (MQ=255)                                                                                                                         cAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTTTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGtt                                                                                       <  2:354944/250‑1 (MQ=255)                                                                                                                           aTACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATTCTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACGTTTTTGATCGCTTTGATATATTACGCGAGTACCCGAAATGACAATGGCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGGTGTATGTTTGGTGTTaaa                                                                                    <  2:210217/251‑1 (MQ=255)                                                                                                                                 gATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTAc                                                                                                                                                                         <  2:174839/160‑1 (MQ=255)                                                                                                                                 gATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTAc                                                                                                                                                                         >  1:174839/1‑160 (MQ=255)                                                                                                                                  aTACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACAt                                                                              >  1:334021/1‑250 (MQ=255)                                                                                                                                     cTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAAc                                                                          >  1:145852/1‑251 (MQ=255)                                                                                                                                      tGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCtgttg                                                                                                    >  1:337807/1‑224 (MQ=255)                                                                                                                                      tGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCtgttg                                                                                                    <  2:337807/224‑1 (MQ=255)                                                                                                                                        gcggctgaGCAGTAAAAGGGCCTGTAATACTGCCAGGCACAGCGTTGTCATCTCCAGTATCTAACCTACTCTGGACAAGCATCGGGTTATTCCCTTTATGTTCCAGACGTGCGTTGACACTGTTGTGATCGCCTTTATATATTACGCGAGTTCCCGAAATGTCAATGTCCGCAATAGACGTTGAACTAAAAGCCATACAGTTTACTAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCAt                                                                       <  1:296275/244‑1 (MQ=255)                                                                                                                                                     aaaaGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGtt                                                          <  1:110180/251‑1 (MQ=255)                                                                                                                                                                                                  aTCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATATACGATGAACTAAAAGCCATACAGGTTACGAAGCAGAAAGCTTTTGTATGTTTGGTGTTAAAAAACATAAACCAGCCCTGATAGAGTTAATGAAGACAGGGAATAATATAAAATAGAAATTATTATTCCCTg              <  1:259888/250‑1 (MQ=255)                                                                                                                                                                                                              ctGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATAttcattca  >  2:293215/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                  TTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCA  >  NC_000913/155823‑156277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑