| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,358,859 | T→C | Y110C (TAT→TGT) | puuP ← | putrescine:H(+) symporter PuuP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,358,859 | 0 | T | C | 93.8% | 92.3 / ‑4.9 | 34 | Y110C (TAT→TGT) | puuP | putrescine:H(+) symporter PuuP |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (17/13); minor base A (1/1); total (20/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
GGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATC > NC_000913/1358618‑1359101 | ggTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCcagca < 1:322760/251‑1 (MQ=255) tctcCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGATGACCAGc < 1:200449/250‑1 (MQ=255) cTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAAACCCACAGCGGAGACCAGCCGAcc > 1:297777/1‑251 (MQ=255) gAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTg < 1:255186/250‑1 (MQ=255) gAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGAACAGGTTGCCGCTCTTCCGATTCGCGGCGGTTAAAATGGCGCCGAAGGTTACCACCCACACCCACGGCGGCACTTACGGGAAGAGGGCGG‑GGAGATAGATTTTCGACAACAA‑GACGTTAATAATCGGCAAAACG‑AGACAAACCAGCACCGCTGACCATCCGCCCATAAATCCgccgcgc > 2:434483/1‑245 (MQ=255) acgaCCAGGAAGAAAAACACCTCCAGGATGGAGATTTGCGCCAGTACAAGCAGGGTATTGAATTTAGCGACCAGGTTGACGCTCGTCAGACTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTGCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCgg < 2:434639/248‑1 (MQ=255) tACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACCCCCCCGGCGGCACTTCCGGGAAGAGGGCGG‑GGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCCGCAGCGATGACCCACCGACCATAAATCCGAcgggcgggg > 1:233487/1‑250 (MQ=255) ccAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTGCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCGCCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTa < 1:77189/251‑1 (MQ=255) aaCACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGAGGACCAGCCGACCATAAATCCGACGTGCGGGTTAAACGACttgtg < 2:384499/251‑4 (MQ=255) caTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGCCGAAGGTTACCCCCCCCACCCACGGCGGCCCTTCCGGGAAGAGGGGGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCCGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTaaagatcggaaaagcgtcgg > 2:272873/1‑233 (MQ=255) caTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTaa < 1:272873/233‑1 (MQ=255) gagaTTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGGGGCACTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAACAGCGATGCACAGCCGACCCTAAATACGACGTGCGGGGTAATCGACTTTTGCGCGTAGGTATAGGc > 1:348243/1‑251 (MQ=255) aCAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCgg < 1:111731/251‑1 (MQ=255) tagagacaaGGTTGACGCTCTTCAGATTCGCGGCGGTTACAATGGCGCAGAAGGATACCACCCACAACCACGGAGGGAATTCCGGGGAGGGGGCGGCAG‑GCTAGATTTTTGCCAAAAC‑GAGGGAAATCACCGGAAAAAAGCAGA‑AATCCAGCAGCGCTGAGCCGCCGACCCTAAATCCGGCGGGCGGGCTCATCAACCTTTGAGCGTAGGTATAGACCGAAACGGACCCCGGGAAATGGCggacacggttg > 2:386897/9‑241 (MQ=255) tctTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGG‑AGAGATAGAATTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGATGAACAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGt > 1:33528/1‑250 (MQ=255) aGATTCGCGGCGGTTAAAATGGCGACGAAGGTTTCCCCCCACACCCACGGCGGCCCTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑AACGTTAATCATCGGCAAAAAG‑AGACCATCCAGCAGCGATGACCAGCCGACCATAAATCCGGCGTGCGGGTTAATCGACTTTTGCGCGGAGGGATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTCGCTGATAACGGGAAAc > 2:255931/1‑249 (MQ=255) ggcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACAcccc > 1:168500/1‑250 (MQ=255) cggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGAGGCACTCACCAGAAGCGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GAAGTTAATCATCGGCAAACAG‑CGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTCCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGCTAGCGGTAAACAGCACACCcgcc > 1:46720/1‑251 (MQ=255) aaaTGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccagcgcc > 1:91050/1‑251 (MQ=255) aaGGTCACCACCCAAACCCACGGCCGCACTTCCGGGAAGAGGCCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCCTCGGCAAAAAG‑CGCAAATCCAGCAGCGATGACCAGACGACCATAAATCCGACGTGCGGGTTAATCGCCTTTTGCGCGAAAGAATAGGCCGAACCGACCTCCGGAAACTGGCGAACCAGTTTGCCGTAACTGATAGGGGTAACCAGCACACCCGCCAGCGCCAGCAAAAAgg > 1:141265/1‑250 (MQ=255) ccggcggacattcggggAAGAGGGCGG‑AAAGATAGTTTTGCGC‑AACAAAGATGTAAATCACCGGAAAAAAG‑AGAAAATCCAGCGGCGAGGACCAGCCGACCATAAATCCGACGTTGGTGTTAATCTATTTTGGCGTGTAGGTAGAGGCCGAACCTGCCTCCGGAAACTGGTGCACCAATTGGCGGTAGCAGACAGCGGTAACCAGCCCAGCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCTTCg < 2:69691/237‑1 (MQ=255) cggcACTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTa > 2:127220/1‑250 (MQ=255) cTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACAcc > 2:248424/1‑202 (MQ=255) cTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACAcc < 1:248424/202‑1 (MQ=255) gCGG‑AGAGATAGATGTTCGCCAAAAA‑GACGTTAAACATCGGAAAAAAG‑AGACAATCCAGAAGCGATGCCCAGCCGACCATAAACCCGACGTGCGGTTTAATCGATTTTTGCGCGGAGGTATAGCCCGACCCGGCCCCCGGAAACTGGCGATCCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCaaa < 1:89358/251‑1 (MQ=255) ggg‑AGAGATAGATTTTCGCAAACAA‑GACGTAAATCACCGGAAAAAAG‑AGACAATCCAGCAGCGATGCCCAGCCGACCATAAAGCCGCCGTGCGGTTTAATCGATTTTTGCGCGTGGGTATAGGCCGACCCGGCCCCCGGAAACTGGCGAACCAGTTTGCCGGAGCTGATAGCGGTAACCAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCaaaa < 2:233487/250‑1 (MQ=255) caacaa‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCg > 1:151347/1‑251 (MQ=255) aa‑GACGTTAATCATCGGCAAAAAG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccagc > 1:370149/1‑171 (MQ=255) cGTTAATCATCGGCAAAAAG‑AGA‑CATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGCGACAATACCAAAAGTATCAAATACGGTCATCGGCGTg > 1:146419/1‑247 (MQ=255) atcGGCAAAAAG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGcc > 1:457747/1‑249 (MQ=255) aaaaG‑AGACAATCCAGCAGCGATGCCCAGCCGACCATAAACCCGACGTGCGGTTTAATCGACTTTTGCGCGTAGGTATGGGCCGACCCGGCCCCCGGAAACGGGCGAACCAGTTTGCCGTAGCGGATGGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCcatc < 1:444021/251‑1 (MQ=255) aaaaG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccagcgc > 1:87354/1‑154 (MQ=255) aaaaG‑AGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGGGACCGTCGCTACTGCCGGAGACAATACCAAAAGTATCAAATACGGTAATCGGAGTGAGATAGGCCAGACCaatc > 2:82776/1‑251 (MQ=255) aaaaG‑AGACAAGCCAGCGGCGATGACCAGCCGCCCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTATCTGTTAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCcatc < 1:32352/251‑1 (MQ=255) | GGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGG‑AGAGATAGATTTTCGCCAACAA‑GACGTTAATCATCGGCAAAAAG‑AGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATC > NC_000913/1358618‑1359101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |