| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,287,040 | G→A | intergenic (+56/+26) | ychS → / ← rttR | putative uncharacterized protein YchS/small RNA RttR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,287,040 | 0 | G | A | 93.3% | 33.6 / ‑3.6 | 15 | intergenic (+56/+26) | ychS/rttR | putative uncharacterized protein YchS/small RNA RttR |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (7/7); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01 | |||||||||||
AACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCG‑CATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGC > NC_000913/1286799‑1287286 | aaCCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCG‑CATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg < 1:188325/251‑1 (MQ=255) ttcgcacccgtacccGTTTGGGCAGAATATTTGATTGCGGATGCGTTTGAGAATTCCGGGTTTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCGGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCG‑CATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGt < 2:217846/238‑1 (MQ=17) ccTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCG‑CATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGAtggtg < 1:372255/250‑1 (MQ=255) aTTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCG‑CATGGGCAGAGTATTTGATTGAGGGTTCGCTTAAGAGTTCAGGGACTTTTGGAAGCGATGGAGGTGGGGGAAGGATTACTCAGCGATGCGCGCTTcc > 2:357698/1‑250 (MQ=17) tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCG‑CATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCACCTCTGCGCGCTTcaccgtt > 2:13764/1‑245 (MQ=255) ccGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCG‑CATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTg > 1:138633/1‑251 (MQ=255) tttGAAAGGGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCG‑CATGGGCAGAATATTTGATTGCGGAGTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCgg < 1:60484/250‑1 (MQ=255) gattatccAGCGCTGCGCCCTCCGCCCTTCGTGTCGTGGCCGGCGGCAACTCTCACCCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCGTCTCAGCCTTCCCCGAGATGG‑CAGAATATTTGATGGCGGATTCGCTTAAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTc < 2:138633/244‑1 (MQ=14) cGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAGCCTTCCCCG‑CATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGc < 1:357698/250‑1 (MQ=255) cGGCAACTCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCG‑CATGGTCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCACCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGaa < 1:33907/251‑1 (MQ=255) tctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCG‑CATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAtt > 1:57313/1‑251 (MQ=255) cTTAGTCGAAGCTTCTCATCCTTCCCCG‑CATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGACTATTTAATTGCGGATTCGTTGGGAAGTTCAggg > 1:255775/1‑250 (MQ=255) cTTCTCATCCTTCCCCG‑CATGGGCCGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCGTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACATTGGACGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAGAGt > 1:176457/1‑251 (MQ=255) ccTTCCCCG‑CTTGGGCAGAATATTTGATTGGGGATTCGATTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggt < 1:45425/249‑1 (MQ=255) gCTTAAGAGTTCAGGGACTTTTGACAGTGATGGTGGCGGGGGAAGGATTACTCAGAGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCCGGCGCTCCAGTCGACCCTTGGTCGAAGCTTCTCATACTTCCCCGCCTGGGCAGAATTTTTACTTTCGAATTCGCTGTGCCGCTCCGCGGTTTTTGATGGTGATGGTGGGGGGGGTATGATTCGATCCTCCGAAGTCGATGACGGc > 2:377627/1‑251 (MQ=9) | AACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCG‑CATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGC > NC_000913/1286799‑1287286 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |