Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	789,580	C→A	E134* (GAA→TAA)	galK ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	789,580	0	C	A	100.0%	94.3 / NA	27	E134* (GAA→TAA)	galK	galactokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/16);  total (11/16)

GTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGTTTTCATGTGCGACAATGGGCGCATCGAGGGAAAACTCGTCGAGCTGATTTTCATAATCGGCTGCCATCACGCGAACTTTACGGTCATCGCGTGGTGCACAACTGATCACG  >  NC_000913/789334‑789824                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     gTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGt                                                                                                                                                                                                                                                  <  2:19627/251‑1 (MQ=255)         ttACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGCGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGACGCgg                                                                                                                                                                                                                                           >  2:332382/1‑250 (MQ=255)           aCTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCGGCAATACGGTTCCGACCGCGACTTACAGTGAAACGgaag                                                                                                                                                                                                                                        <  1:119269/251‑1 (MQ=255)             tGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGgaaga                                                                                                                                                                                                                                       >  2:333998/1‑250 (MQ=255)                      tgaCGACAGCCACACCTTTGCGCATGGAACCTGCTTTGGTACCCAGGGAGCGGCAATCGATCAGCAAGGCATGCTCTTTCTTGCCGAGCGCGGCAATTAGCTGATACATGATCCCGCAGTTACAGCCTACACACTGGTTTTCTGCTTCCTGACAGTTACGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCACTAGGGTTCCGCCAGCGACTTACAGTGAAGCGGAAGCACTTACCCCg                                                                                                                                                                                                                             >  2:253528/1‑251 (MQ=255)                       gaggacAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCg                                                                                                                                                                                                                             <  2:244659/247‑1 (MQ=255)                          gacAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCAc                                                                                                                                                                                                                         >  2:381256/1‑251 (MQ=255)                                                               cccAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCAcgcc                                                                                                                                                                                    <  2:371882/251‑1 (MQ=255)                                                                   gTGAGCGGCAATCGAGCAGCAAGGCATGAGCTTTCTTGCCGGGCGCGGAAAGTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCGGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTTAGCACCATGGCCACGccgccg                                                                                                                                                                                <  2:409496/251‑1 (MQ=255)                                                                       gCGGCAATCGATCAGCAAGTCATGATCTTTCGTGCCGTGCGCGGAAATTATCTTATCCATGATCCCGCAGTTACAGCCTTCAAGCTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATGCGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTTCCGCTGATCACCATGTCCACGCCGCCGAAGc                                                                                                                                                                            <  1:459239/251‑1 (MQ=255)                                                                        cGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTGGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCt                                                                                                                                                                           <  1:447333/251‑1 (MQ=255)                                                                                      cAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGtt                                                                                                                                                             <  1:333998/251‑1 (MQ=255)                                                                                         ggCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGc                                                                                                                                                           >  2:371053/1‑250 (MQ=255)                                                                                                       tGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTcaccac                                                                                                                                             <  1:381256/250‑1 (MQ=255)                                                                                                        gCCGAGCGCGGAAATTAGCTGAGCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCAccacg                                                                                                                                            <  2:114337/250‑1 (MQ=255)                                                                                                            aGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAGGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCAccacgccacg                                                                                                                                       >  1:253570/1‑251 (MQ=255)                                                                                                               gcgGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAAc                                                                                                                                    <  1:43133/251‑1 (MQ=255)                                                                                                                          cTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGccc                                                                                                                         >  1:86807/1‑251 (MQ=255)                                                                                                                                cATGACCCCGCAGTTACAGCCTACAAACTGGCTTTCTGCTTACACACAGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCCGCTGTTAAACCTGCTGCAATACGACTCCGACCGCGACTTACAGTGAAGCGGACGAAATTAACCCGGCACCCAGCGGCACATTGCCCCTGCTCACCATGTCCACGCCGCCGAAGCTGTTGATACGCAGTTGCAGATGTTTCACCACGCCACGAACGCAGTTAGCACATTGAt                                                                                                                   >  1:451075/1‑251 (MQ=255)                                                                                                                                 aTGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATa                                                                                                                  <  1:290287/251‑1 (MQ=255)                                                                                                                                    aTCCAGCAGGTACAGCCTACACACGGGTTTTCTGCTTCCTGTCCGTGAAGCGCTATTGTTACGACATGCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACCTAACCCGGCACCCTGGGGCAGATTGTCTCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGTAGTTGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGtt                                                                                                               <  1:387163/251‑1 (MQ=255)                                                                                                                                                                    tGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACCTTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGTTTTCATGTGCGACAATGGGCGCATCGAGGGaa                                                                                >  2:401649/1‑250 (MQ=255)                                                                                                                                                                                       gcgATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGTTTTCATGTGCGACAATGGGCGCATCGAGGAAAAACTCGTCGAGCTGATTTTc                                                            >  2:344897/1‑251 (MQ=255)                                                                                                                                                                                                        cAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTACCCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGTTTTCATGTGCGACAATGGGCGCATCGAGGGAAAACTCGTCGAGCTGATTTTCATAATCGGCTGCCATCa                                           <  1:334860/251‑1 (MQ=255)                                                                                                                                                                                                                      aaaGCTGCTGCAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGTTTTCATGTGCGACAATGGGCGCATCGAGGGAAAACTCGTCGAGCTGATTTTCATAATCGGCTGCCATCACGCGAACTTTACg                              <  1:332382/250‑1 (MQ=255)                                                                                                                                                                                                                                                 cgACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGTTTTCATGTGCGACAATGGGCGCATCGAGGGAAAACTCGTCGAGCTGATTTTCATAATCGGCTGCCATCACGCGAACTTTACGGTCATCGCGTGGTGCACAACTGATCACg  <  1:337100/251‑1 (MQ=255)                                                                                                                                                                                                                                                  gACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGTTTTCATGTGCGACAATGGGCGCATCGAGGGAAAACTCGTCGAGCTGATTTTCATAATc                                                      >  1:442577/1‑198 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     GTTTGAAGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGTTTTCATGTGCGACAATGGGCGCATCGAGGGAAAACTCGTCGAGCTGATTTTCATAATCGGCTGCCATCACGCGAACTTTACGGTCATCGCGTGGTGCACAACTGATCACG  >  NC_000913/789334‑789824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑