| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 377,423 | 0 | G | T | 38.7% | 60.7 / 68.3 | 62 | intergenic (+112/+112) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB |
| Reads supporting (aligned to +/- strand): ref base G (20/18); new base T (14/10); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.50e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAAC‑TGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTC > NC_000913/377187‑377669 | ttGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGcc > 1:414685/1‑251 (MQ=255) ccGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTg > 2:400258/1‑250 (MQ=255) cGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCGTCCGGCATTTGTgcgctgatgcctg > 1:217272/1‑241 (MQ=255) aaTTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCACAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACg < 1:319169/251‑1 (MQ=255) tCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTgc > 1:231546/1‑251 (MQ=255) cAGCGCGATGCCAGCAGTATAGTGTTGCACACAGCGCTCAGCGCCGACGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGCTGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCtgacg > 1:448783/1‑247 (MQ=38) aGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCtgacgc < 1:321044/251‑6 (MQ=38) cgATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGGGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGACGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCtgacgcgtct > 1:336010/1‑242 (MQ=38) ataccAGCAGTATAGTGATGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCACAGTGCCTGATGATTTGATGTGGTAAACATAGATAAATCGTGCGCAGATGCCAGATGCGCCGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATCAGGCGTTC‑ACGCCGCATCCGGCAGTCATGCGC‑C‑ATACCTGATGCGACGCTTTGCGCGTCtt > 2:315539/4‑250 (MQ=255) gcagTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCatgc > 1:238767/1‑248 (MQ=37) cagTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGcc < 2:301043/251‑1 (MQ=37) cagTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGcc > 1:46228/1‑251 (MQ=37) gTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCt > 1:226031/1‑250 (MQ=37) ataGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAc > 1:115675/1‑250 (MQ=37) ataGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCATATCATGCCTAc < 1:12194/250‑1 (MQ=37) ataGTGTTGAACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACg > 2:290791/1‑251 (MQ=255) gtgtTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGAGGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTGATGGGCTAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATTCGACGCGG‑ACGCGTCTTATCATGCCTAcaaat < 2:448783/251‑5 (MQ=25) ggtTGCACAGCGCGCTCAGCGCCGAAGAGCAGGAGGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATGTGATGTGGTAAACATAGATAAGTCGTTCTCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCt‑t < 2:231546/250‑1 (MQ=255) gtTGCACAGCGCGCGCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTGCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCt‑t < 1:98586/250‑1 (MQ=255) tGCACAGCGCGCTCAGCGGCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCGGAGGATTTGATGTGGTAAACAGAGAGAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctg < 2:86046/250‑8 (MQ=21) gcTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacgcgaaccg > 1:78891/1‑233 (MQ=21) gcTCAGCGACAAAGAGTAGGATGAATGCGGCCCGTATGCCTAGTTCAACGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCTCATATGCCGGATGCTACGCTATGCGCGTCTTCTCATGATTACGGGTTATTGTCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCTTCCGGCTTTTGTGCGCTG‑ATGCCTGAGGCGACGCTG‑ACGCGTCTTATCATGCCTActtatctgtactcgaatct < 2:85960/251‑19 (MQ=255) cTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGAACGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacgcgaaccg > 2:364456/1‑232 (MQ=21) cAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGGGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTTCGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGC‑TTATGCCTGATGCGAC‑CCTGACGCGTCTTATCATGCCTAcaaatctgtacgcgaaccgtag < 1:328793/251‑22 (MQ=21) cAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTACAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacgcgaaccgt > 1:390149/1‑230 (MQ=21) aGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacgcgaaccgtagg < 1:146486/251‑23 (MQ=21) gcgcCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGGGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatagtccgaaccgtaggccgg < 2:131173/251‑24 (MQ=21) gcCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCgg < 1:168489/251‑1 (MQ=255) gAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGAt > 1:173032/1‑250 (MQ=255) aagccgacCCGTGTGCCGACTTCAGAGTGCCTGAGGATTTGAGGTGGTAAACATAGACAAGTCGTTCTCAGATGCCTGATGCTACGCTATGCGCGTCTTATCGGGCCTACGGTTGATGGGCGAATTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTTCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCAcgcc < 2:142456/247‑1 (MQ=37) cGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTACAAATCTGTACG‑‑CGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCg > 2:222855/1‑250 (MQ=14) gTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 2:339496/1‑250 (MQ=255) aTGCCGACTTCAAAGTGCCTGAGGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCGGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTACAAATCTGTACG‑‑CGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCgacct < 2:146633/251‑5 (MQ=2) gCCGACTTCAAAGTGCATGATGATTTGATGTGGTAAACATAGATAAGTAGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATAGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGAGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg > 1:262537/1‑251 (MQ=255) gACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTACAAATCTGTACG‑‑CGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCgacctgaaa > 1:403594/1‑242 (MQ=2) cAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTACAAATCTGTACG‑‑CGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCgacctgaaaattctt > 2:98161/1‑237 (MQ=2) aaaGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTT‑ACGCCGCATCCGGCATTTGTGCGCTG‑ATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTACAAATCTGTACG‑‑CGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCgacctgaaaattctt > 1:190325/1‑236 (MQ=2) ccTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGc > 2:36818/1‑251 (MQ=255) gatgatTTGATGTGGTAAACATAGATAAGTCGTGCGCAGTTGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAGGTTTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCat < 1:401561/250‑1 (MQ=255) gtgGTATACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTAtt > 1:118187/1‑251 (MQ=255) ggTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCCGTCGTGCTATTATCAACGCATATTCAGTTTATTgg > 2:90030/1‑251 (MQ=255) aTAGATTAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGt > 1:130216/1‑250 (MQ=255) gTCGTGGGCAGATGCCTGATGCGACGCTATGCGCGGTTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAatat < 2:222019/251‑1 (MQ=255) gTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAatat < 1:290791/251‑1 (MQ=255) cGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTGC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGc < 1:364235/251‑1 (MQ=255) cGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAAC‑Tgg > 1:211307/1‑248 (MQ=255) aTGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAAC‑TGGAGACAAaata < 1:280538/251‑1 (MQ=255) cgcgTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCATTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAAC‑TGGAGACAAAataata < 2:173032/251‑1 (MQ=255) gTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAAC‑TGGAGACAAAATAATGGCt > 1:158597/1‑251 (MQ=255) ttATCAGGCCTACGGTTTATGGGCGAAGTGTAAACAGCATAAGCCGTTA‑ACCCCGCCTCCGGCAGTCGAGCGC‑C‑ATGCCTGATGCGACGATTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TAAGGGCGAAGTGTACGCCCGATAAGGCGTTAACGCCGCATCCGGCAGTCGCGCTATTATCAACGCATATTCAGTTTATTGGCGTGGAAGCCAATATGCACGCCAATAAAAC‑TGGAGACAAAATAATAGCtggg > 1:454310/1‑249 (MQ=255) aCGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAAC‑TGGAGACAAAATAATAGCTGTGATCATAACCCt < 1:36818/251‑1 (MQ=255) aCGGTTTATGGGCGAAGTGAAAACCGGATAAGGC‑TTCTACGCCGCATCCGGCAGTCTTGCGC‑C‑ATGCTTGATGCGACGCGTTGCGCGGCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCTCCAATAAAAC‑TGGAGACATAATAATAGCTGTGATCATAAccc < 2:421716/250‑1 (MQ=38) ttATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGAGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAAC‑TGGAGACAAAATAATAGCTGTGATCATAACCCTCGTg > 2:119042/1‑250 (MQ=255) ttATGGGAGAAGTGTAGACCGGAAAAGGCGTTC‑ACGCCGCATCCGGCAGGCGGGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTAGCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAAC‑TGGAGACAAAATAATAGCTGTGATCATAACCCTCGTg < 2:17117/250‑1 (MQ=255) gggagAAGTGAAGCCCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTTTAGGCCGGATAAGGCGGTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAAC‑TGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAAc < 2:153386/247‑1 (MQ=255) aGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAAC‑TGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGAtt > 1:228067/1‑250 (MQ=255) aGACCGGATAGGGCGTTC‑ACGCCGCATCCGGCAGGCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAAC‑TGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGt < 2:130216/250‑1 (MQ=255) gACCGGAGAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCGTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAAC‑TGGAGACAAAATAAGAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCt < 1:60930/251‑1 (MQ=255) aTAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGA‑C‑AAGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TCCGGGCGAAATGTAGGCCGGATAAGGCGTTCACGCCGAATCCGGCAGACGAGCTATTATCAACACCTATTCAGTTTTTTGGCGCAGTAGGAGACATGCTCGGCAATAACACATGGGTACCAA‑TACAAGCTTTGATTCTATCCCTCGTGATAACGGATTATGGTCTTGatat > 2:79319/1‑251 (MQ=16) aTCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATCTTCAGTTTATTGGCGTGGTCGGCAAAATGCTCGCCAATAAAAC‑TGGAGCCAAAATTATAGCTGGGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTTGCAGATCt > 2:179752/1‑251 (MQ=38) gtctgTCGTGGGC‑C‑ATGCCTGATTGGACGCTTTTCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTGCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAAC‑TGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTc < 2:329857/247‑1 (MQ=255) cAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAg < 1:102272/145‑1 (MQ=255) cAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAg > 2:102272/1‑145 (MQ=255) | TTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTC‑ACGCCGCATCCGGCAGTCGTGCGC‑C‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAAC‑TGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTC > NC_000913/377187‑377669 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |