| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 354,288 | 0 | T | G | 25.7% | 139.5 / 10.4 | 71 | V528G (GTG→GGG) | prpE | propionyl‑CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (6/46); new base G (18/0); total (25/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.80e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGA > NC_000913/354038‑354536 | ccGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑t < 2:340145/251‑1 (MQ=255) ggcgccgGGGTGTATTCAGAACATCTGGGGGGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACGGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑aaa < 2:246209/248‑1 (MQ=255) gccgccGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGTG‑TG‑AAAg < 1:270266/251‑1 (MQ=255) ggggTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATgcgc < 1:280181/251‑1 (MQ=255) gggTGTATTCAGACCATCTGGGGCGACGACGGCCGCTGTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCGTTGACTGGGGCATCCGGGACGCTGACGGTTAGCACTTTATACGCGGGCGCACTGACGATGTAATTAGCGTTGGCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGGATGTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCt < 1:206571/251‑1 (MQ=255) ggTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTg < 1:339788/251‑1 (MQ=255) cAGACCATCGGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCa < 2:305498/251‑1 (MQ=255) aGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAg < 2:142613/251‑1 (MQ=255) aGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACGGGTCGCTGTTTTCCCGCCCGGTGTACGCCTCCTGTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCGGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCAGCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATTCGCTG‑AAA‑GGGCAg < 2:55631/251‑1 (MQ=255) gggggcggagacgGCCGGTTTGTGAAGTCTTACGGGTCTCTGGTTTCCCGCCCGGGGTACGCCACCTTTGACTGGGGTATCCGTGACGCTGACGGTTATCACTTTAGTCTCGGGCGCGCGGACGATGTAATTAACGTTGCCGGGCATCGGCGGGGGACGCGCGAGATTGAAGGGAGTATCTCCAGCCAACCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGAGGCGCTG‑AAA‑GGGCAggtggcggt < 2:281423/242‑1 (MQ=255) gCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCAtt < 1:391979/251‑1 (MQ=255) gtgAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAag < 1:394036/250‑1 (MQ=255) tgAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑GG‑AAAGAAGCGCTG‑AAA‑GGGGAGGGGGGGGGGGCG‑TTTGTCATTTCGAAag > 1:449051/1‑249 (MQ=255) ggTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCt < 1:26310/250‑1 (MQ=255) cGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGa < 1:222803/250‑1 (MQ=255) ttttCACGCCCGGTGTACGCCACCTTTGACTGGCGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGCGATTGCAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑GAAGAAGCGCAGCAAA‑GGGAAGG‑GGCGGGAGGG‑CTTGTAATTCCGAAAAAGAACGACAGtattggagat > 1:232669/1‑240 (MQ=255) ttCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑GGGAAAGA‑GCGCTG‑GAA‑GGGGAGGGGGCGGGGGCG‑GTTGTCATTTCGAAAGAGAGCGGCAGGCTGGGAGATCGTg > 1:212248/1‑251 (MQ=255) tCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGG‑GTGGGG‑GG‑GAAGATGCGCTG‑AAA‑GGGCAGGGGGCGGGGGCG‑GTTGTCCTTCCGAAAGAGAGCGACAGTCTGGGAGAGCGTgg > 1:285281/1‑250 (MQ=255) cccgcccgGTGTACGCCACCTTTGGCGGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGTCAGTCTGTAAGATCGTGAt < 2:288324/251‑1 (MQ=255) cccgcccgGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAt < 2:339448/251‑1 (MQ=255) ccgcccgGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtg < 1:365416/251‑1 (MQ=255) cccgTTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGGAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCCGCCATCCGGGCGTTGCCGAAGTGGCGGGGG‑TTGGGGGTG‑AC‑GATGCACGG‑AAAAGGGC‑GGAGGCGGTGGCG‑TTTGTTATTCCGAAAGAAGGCGACCGTCTGGGACATCGTGAtgggg > 2:313303/1‑248 (MQ=255) ccgGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGG‑GG‑GAAGATGCGCCG‑AAA‑GGGCAGGTGGCGGGGGCG‑GTTGTCATTCCGCAAGAGCGCGGCAGTCTGGGAGAGCGTGGTGTggg > 2:51255/1‑250 (MQ=255) ggTGGACGCCACCTTTGACTGGGGCAGCCGTGACGCTGACGGTTATCACTTTATGCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGTGACGCGCGAGATTTAAGAGAGTATCGCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GTGCAGGTGGCGGTGGCG‑TTTGTCATGCCGAAAGAGAGCTATAGTCTTGAAGATCGTGATGGGgctc < 2:232669/251‑3 (MQ=255) tgtACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGG‑GG‑GAAGATGGGCTG‑AAA‑GGGCAGGGGGCGGGGGGG‑GTTGTCCTTCCGGAAGAGAGCGGCCGGCTGGGAGATCggggtggggcgccc > 1:168424/1‑238 (MQ=255) gtACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGGAGTGGCGGGGG‑GTGGGG‑GG‑GAAGATGAGCCG‑AGA‑GGGCCGGTGGCGGGGGCGGTTTG‑CACTCCGGAAGAAAGGGCAAGTCTGGGAGATAGTGGTGTGGGGCAcc > 2:142582/1‑250 (MQ=255) gCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGG‑GG‑GAAGATGCGCTG‑GAA‑GGGCAGGGGGGGGGGGGG‑TTTTTCCTTTCGAAAGAGGGCGGCAGTCTGGGAGATCGTGGTGTGGGGCCCTCGcc > 1:262945/1‑250 (MQ=255) ccTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGAGTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCAGTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag < 1:49896/251‑1 (MQ=255) ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt < 1:139642/251‑1 (MQ=255) aTCCGTGACGCTGATGGTTATCACTTTATTCCCGGGCGTACTGACGATGGAATTAACGTTGCCGGGCATCGGCTGGGGCCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGTCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgc < 1:240250/251‑1 (MQ=255) gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtg < 1:262339/249‑1 (MQ=255) gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGACGCAATTAACCTTGCCGGGCATCGGCTGGGGACGCGCGAGCTTGCAGAACGTATATCCCGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTTGGGG‑‑G‑AAAGAGGCGCTG‑GAA‑GGGGCGGGGGCGGTGGCG‑TGTGGCGTTCCGAAAGAGAGCGGCAGGATTGAAGATCGGGATCGGGCGCAATCGCAAGGGAAgtggcatatggtgctggtggc > 1:39716/1‑231 (MQ=255) tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGG‑GG‑GAAGATGGGCTG‑GAA‑GGGCCGGGGGGGGGGGGG‑GTTGTCATTCCGGAAGAGAGCGGAAGGGTGGGAGAGCGTGATGTGGGGGGCTCGCAAGaggagggggtttttgcggaggtggac > 1:97281/1‑230 (MQ=255) gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGAGTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa < 2:409281/250‑1 (MQ=255) cGGGCGCACTGACGATGGAATTAACGTTGCCGTGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATTCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGATAGCTACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc < 1:150154/251‑1 (MQ=255) cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑GG‑GAAGATGCGCTG‑GAA‑AGGCAGGTGGCGGGGGCG‑GTTGTCATTCCGAAAGAGAGCGAAAGTCTGGAAGATCGGGATGGGGCGCAACCGCAAGAGAAAGCGATTATGAGGCTGGGGGGCAGCCCAATTGGGCACATTGGGCGCCCgg > 1:267785/1‑251 (MQ=255) gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGt < 2:446839/251‑1 (MQ=255) aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑GG‑AAAGATGCGCTG‑AAA‑GGGAAGGTGGCGGGGGGG‑GTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCCGATTGGCAACATTGGCCGCCCGgcgcccggc > 1:438398/1‑246 (MQ=255) aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTgg < 1:170278/250‑1 (MQ=255) agaaCGTTGCCGGGCATCGGCGTGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCAGCCGGGCGTTGCCGGAGGGGCGGTGG‑TTGGGG‑TG‑AAAGAATCGCTG‑AAA‑GTGCAGGTGGCGGTAGCT‑GTTGTCAATCCGCAAGAGAGCGACAGTCTCGAAGATCGTGATGTGTCGCACACGCAAGAGAAGGCGAGTATGTCGCTGGTGGACAGCCAGATGTGCAACTTCGTCCGCTCGGCGCCCGTcgagttt < 2:369444/249‑7 (MQ=255) ggggCCGGGCATCGGCTGGTGACGCGCGAGATTGAAGAGAGTAGCTCCAGCCAGCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCGG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAGCGTGATGTGGCGCACGCGCAAGAGAAGGCGATTAGGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGGCGGGCGCACGTCTGGTTTGtctc < 2:31902/248‑1 (MQ=255) gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGtctc < 1:340059/251‑1 (MQ=255) gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTTGGG‑GG‑AAAGATGCGCTG‑AAA‑AGGCAGGTGGCGGGGGGG‑TTTTTTATTCCGAAAAAGAGCGGCAGTCTGGGAGAATGTGATGTGGGGCCCTCGCCAGAGAAGGCGGTTATGGCGCCGGTGGACAGCCCGATTGGCAACTTTTGCCCCCCGGCGCACGTCCGGTTTGTCTTGCAA‑tt > 1:413768/1‑251 (MQ=255) gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑tt < 2:168424/251‑1 (MQ=255) ggtgggggACGCGCGAGAGTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑aaaa < 2:265476/247‑1 (MQ=255) gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑GG‑AAAGATGCGCTG‑GAA‑GGGCAGGGGGCGGGGGCG‑GTTGTCATTTCGAAAGAGAGGGACAGTCTGGAAGATCGTGGTGTGGGGCACTCGCAAGAGAAGGGGGTTATGGGGCTGGGGGGCAGCCAGATTGGGAACTTTGGCCGCCCGGGGCCCGGCTGGGTTGTCTCGAAA‑TTGCC‑aaaa > 1:193938/1‑250 (MQ=255) gggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGG‑GG‑AAAGATGCGCTG‑AAA‑GGGGAGGTGGCGGGGGGG‑GTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTGGGGCACTCGCAAGAGAAGGCGATTATGGGGCTGGGGGACAGCCAGGTTGGCAACTTTTGCCGCCCGGCGCCCGTCTGGGTTGTCTCCCAA‑TTGCC‑AAAAAagcg > 1:438408/1‑251 (MQ=255) gACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAACGCGAt < 1:197831/251‑1 (MQ=255) cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTg < 2:338856/136‑1 (MQ=255) cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGG‑TG‑AAAAATGCGCTG‑AAA‑GGGCAGGGGGGGGGGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGGCAGCCAGATTGGCAACTTTTGCCCCCCGGCGCACGGCCGGTTTGTCTCGCCA‑TTGCCCAAAAAC‑CGATcccg > 1:328890/1‑249 (MQ=255) cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGG‑GG‑AAAGATGCGCTG‑GAA‑GGGCAGGTGGCGGTGGCG‑GTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTg > 1:338856/1‑136 (MQ=255) gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGG‑GG‑AAAACAGCGCTGGAAA‑GGG‑AGGTGGGGGGGGCG‑GTTGTTAATCCGGAAAAGAGGGGCAGGCCGGAAGAACGTGATGGGGGGCAACCCCAAGAGAAGGCGATTATTGGGCTGGTTGACAGCCCGAGTGGCAACTTTTGCCCCCCCGCGCACGTCTGGTTTTTCTCGCAAATTCCC‑AAAAAC‑CGATcccggc > 2:400290/1‑246 (MQ=255) gaTTGAAGATAGTATCTCCAGCCATCCGGGCGTTGTCGAAGTGGCGGGGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGGTCTTGATGTGGCGCACTCGTAAGAGAAGGCGATTGTGGCTCTGGTTGACAGCTGGATTGTCAACTTTGGCGGCCCGGCGCACGTCGGGTTTGTCACGCAA‑TTGCC‑AAAAACGCGATCCGGaaaaa < 1:67164/251‑1 (MQ=255) gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAACGCGATCCGGaaaaa < 1:51255/251‑1 (MQ=255) aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAACGCGATCCGGAAAAAt < 1:410580/251‑1 (MQ=255) gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTTG‑TTTGGG‑GG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGGGGCG‑TTTGTCATTCCGAAAGAGAGCGGCAGTCTGGAAGATCGGGGTGTGGCGCCCTCGCCAAAGAAGGCGGTTATGGCGCTGGGGGACAGCCAGATTGGCCACTTTGGCCGCCCCGCGCACCTCTGGGTTGTCTCGCAA‑TTTCC‑CCAAAAGCGATACGGAAAAAtact > 2:116057/1‑248 (MQ=255) gagagGATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGATCGACAGTCTGGAAGAGCGTGATGTGGCGCACTCGCAAGAGAAGGCGAGTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACCTCTGGTTTGGCTCGCAA‑TTGCC‑AAAAACGCGATCCGGAAAAATGCTgcg < 2:97281/251‑1 (MQ=255) agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGTAA‑TTGCC‑AAAAACGCGATCCGGAAAAATGCTgcgc < 1:716/251‑1 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt < 1:177903/153‑1 (MQ=255) tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGAAGCGCTG‑AAA‑GGGGAGGGGGCGGGGGGG‑TTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGGCGCACTCGCAAAAGAAGGGGGTTATGGCGCTGGGGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAAAGCGATCCGGGAAAAAGCTGcgccgc > 1:162009/1‑250 (MQ=255) tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑GG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGGGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCGGTTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCCGCGCACGTCTGGTTTGTCTCGCAA‑TTTCC‑AAAAACGCGATCCGGAAAAATGCTGcgccgcc > 1:91252/1‑250 (MQ=255) ccAGCCATCCGGGAGTGTCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATGCCGAAAGAGAGCGACAGTCTGGAAGAGCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAACGCGAGCCGGAAAAATGCTGCGTCGCAc < 2:17810/247‑1 (MQ=255) cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TGGCC‑AAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATcc < 2:262945/251‑1 (MQ=255) ccATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑GG‑GAAGATGCGCTG‑AAA‑GGGCAGGGGGCGGTGGCG‑GTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGAACGTGGTGGGGCGCCCCCGCCAGAGAAGGCGGTTATGGCGCTTGTGGGCAGCCAGAGTGGGAACTTTGGCCGCCCGGCGCCCGTCCGGTTTTTTTCGCCA‑TTTCC‑CAAAAAGCGAGCCGGAAAAATAATGCGCCGCCCGAACCAgg > 2:317740/1‑251 (MQ=255) cGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAACGCTAGCCGGAAAAATGCTGCGCCGCGCGATCCAGGCGAtt < 1:353204/251‑1 (MQ=255) tGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGaa < 1:83255/250‑1 (MQ=255) ggCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAg < 2:89778/250‑1 (MQ=255) gCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATcc > 1:410588/1‑251 (MQ=255) cGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCt < 2:38199/251‑1 (MQ=255) ggtgg‑tTGGGA‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTAGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAAGGCGCTGGACGTAAGCCAGCTTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAACGCGATCCGGAAAAATGCTGCGCCGCAAGCTCCAGGCGATTTGCGAAGGAAGCGATCCTg > 1:304937/1‑251 (MQ=255) gg‑tTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAGCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCTGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGgag < 1:276367/251‑1 (MQ=255) gg‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAACGCGATCCGGAAAAAGGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTga < 1:192509/251‑1 (MQ=255) | CCGCTGCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGG‑TG‑AAAGATGCGCTG‑AAA‑GGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAA‑TTGCC‑AAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGA > NC_000913/354038‑354536 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |