Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	246,747	0	G	T	44.6%	28.7 / 79.5	65	S12S (TCG→TCT)	yafL	NlpC/P60 family protein YafL
Reads supporting (aligned to +/- strand):  ref base G (22/14);  new base T (15/14);  total (37/28)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.31e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GGGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCTCAAGG  >  NC_000913/246504‑246994                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        ggggTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGTTTAc                                                                                                                                                                                                                                                  >  1:282436/1‑251 (MQ=255)        ctcCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGAt                                                                                                                                                                                                                                           >  1:232565/1‑251 (MQ=255)         tcCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGAtt                                                                                                                                                                                                                                          >  1:155491/1‑251 (MQ=255)          ccTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGa                                                                                                                                                                                                                                            <  2:415479/248‑1 (MQ=255)          ccTGTGCTGTATATTTATTCAGCTTGAATTGTAGCGCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAAGAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATGCACTGCAACTTTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGAtt                                                                                                                                                                                                                                          <  1:365557/250‑1 (MQ=255)          ccTGTGCTGTATATTTATTCAGCTGGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATAGATATGTTAATTTTATAATAATTGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGTCCAGCCATTATTTAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTg                                                                                                                                                                                                                                             <  2:375032/247‑1 (MQ=255)             gtgCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGAtttgtt                                                                                                                                                                                                                                      <  1:3647/251‑1 (MQ=255)              tgCTGTATATTTATTCAGCTTGAATTGTAGCAAAAATGCGCAACAGAGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGAtttgttt                                                                                                                                                                                                                                     >  1:271717/1‑251 (MQ=255)               gCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACGGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGAtttgttt                                                                                                                                                                                                                                     <  2:352052/250‑1 (MQ=255)                  gTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACAAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCg                                                                                                                                                                                                                                 >  1:385294/1‑251 (MQ=255)                    atatTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGtt                                                                                                                                                                                                                               <  1:357486/251‑1 (MQ=255)                    atatTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTGAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGtt                                                                                                                                                                                                                               >  1:177341/1‑251 (MQ=255)                          attCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTttctt                                                                                                                                                                                                                          <  1:32870/250‑1 (MQ=255)                                         tAGCTAAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGCTACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCCTTTGCCAGCGccac                                                                                                                                                                                                          >  2:370277/1‑251 (MQ=255)                                           gCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGAtttgttt                                                                                                                                                                                                                                     <  1:13714/222‑1 (MQ=255)                                           gCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGAtttgttt                                                                                                                                                                                                                                     >  2:13714/1‑222 (MQ=255)                                                        aCAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATAtctc                                                                                                                                                                                           <  1:103189/251‑1 (MQ=255)                                                             gACAATCCATGAGTAATTTAATAAACGGCTGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTc                                                                                                                                                                                       >  2:453981/1‑250 (MQ=255)                                                             gACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTc                                                                                                                                                                                       >  2:108813/1‑250 (MQ=255)                                                                     aTGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTAcgcc                                                                                                                                                                              >  1:169836/1‑251 (MQ=255)                                                                         gTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGccgccc                                                                                                                                                                          <  1:453981/251‑1 (MQ=255)                                                                         gTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGccgccc                                                                                                                                                                          >  2:122286/1‑251 (MQ=255)                                                                                   taaCGTATGTTTTTACTTAATTAACTGAAATATATATGTAAATTTTTTAATAATGGTTTATTAAGTTTGTTCGAAAACT‑ACCCGGGATACACTGCAACATTAAATATTGCCCAGCATTTATTGAGTATTTCCTTGCCGTCTATTTCATCCTTTTTATTGTCTGGATTACTTTTGTTTTGTTTCCCGTTGTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTCCGCCGCCCGCCTGCggt                                                                                                                                                                 <  2:310890/249‑2 (MQ=255)                                                                                    caCGGATGTTTTTACATAATTAACTGAAATATATTTGTTAATCTTATCATCTTGATTTCTTAAGTTTGTTCAGAAACG‑ATCAAGGATCCGCTGCACCCTTAATGCATGACCAGCCCTTATTGAGAGTATACTTGTCGAAGATTCCATCCTTTGTATTGTCTAGATTACTGTTGGTATGTTTGCCGTTTTCTTCATTTGACAGCGCAACCACATCACATATCTCTTTCAGCTCCGCAGCCCGCCACCGGATg                                                                                                                                                               >  1:57873/2‑251 (MQ=255)                                                                                    aaCGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATg                                                                                                                                                               >  2:4594/1‑251 (MQ=255)                                                                                          tGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAAc                                                                                                                                                         >  1:155781/1‑251 (MQ=255)                                                                                            tttttACATATTTAACGGAAATAGATTTGTTAATTTTAAAATAAGGGATTATTAATTTCGGCCAGAA‑CGTATCCGGTATACGCAAGAACATAAAAGTATCCCCAGCCGTTATGGAGTATGTCGTGGCCGTCGCTTCCTTCCTTTGTATTGTCGGGATTACTGTTGATTGGTTTGCCGTTTTCTGCCTGTGCCAGCGCCACCACAACCCATACCTCTTTCATCTACGCCGCCCGCCAGCGGATGCAAAAcc                                                                                                                                                        <  2:211504/250‑1 (MQ=255)                                                                                                              aaaTATATATGTTACTTTTATAATAATGGTTTATTAAGTTTGTTAAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGCGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACTCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTTTTaaacc                                                                                                                                      >  2:268653/1‑248 (MQ=255)                                                                                                                 tatatatGTTAATTTTATAATAATGGTTTATTCAGTTTGTTCAGAAAAG‑ATCCGGGATACACTGCAACATTAAAGCATGAACAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCCGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGt                                                                                                                                   >  2:174105/1‑250 (MQ=255)                                                                                                                     tatGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCa                                                                                                                               >  1:254892/1‑250 (MQ=255)                                                                                                                           aaTTTTATAATAATGTTTTTTTAAGTTTGTTCAGAAACG‑ATCCTGGATACACTGAAACTTAAAAGCTTGTCTAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCTTTTTCTTCTTTTGCCAGCGCCACCTCATCACATAGCTCTTTCAGCTCCGCCTGCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCa                                                                                                                         <  1:270442/250‑1 (MQ=255)                                                                                                                             ttttATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTTCGCGTTTATTCAAACAGTACCAAACTCATCt                                                                                                                      >  2:249935/1‑251 (MQ=255)                                                                                                                               ttATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTg                                                                                                                     >  2:61720/1‑250 (MQ=255)                                                                                                                                  taataaTGGTTTATTAAGTTTGTTCAGAAACG‑CTCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaa                                                                                                                  >  1:445442/1‑250 (MQ=255)                                                                                                                                  taataaTGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTCTTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaa                                                                                                                  >  1:214147/1‑250 (MQ=255)                                                                                                                                  taataaTGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAACCTCATCTGaaa                                                                                                                  >  2:34229/1‑250 (MQ=255)                                                                                                                                   aataatGGTTTATTAAGTTTTTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACTAAACTCATCTGaaaa                                                                                                                 <  1:92710/250‑1 (MQ=255)                                                                                                                                   aataatGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaaaa                                                                                                                <  1:250668/251‑1 (MQ=255)                                                                                                                                   aataatGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaaaa                                                                                                                <  1:311398/251‑1 (MQ=255)                                                                                                                                     taatGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGTTTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAACTACGCCGCCCGCCAGCGGATGCACAACCGTGCCCGTTTATTAAACCCGTACCAAACTCATCTGAAAAAGc                                                                                                              >  2:377988/1‑251 (MQ=255)                                                                                                                                                  aGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGCTTCCATACTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAACCTCATCTGAAAAAGCCGGCCAGCTatat                                                                                                 >  2:205615/1‑251 (MQ=255)                                                                                                                                                   gTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATAtt                                                                                                >  1:193757/1‑251 (MQ=255)                                                                                                                                                      tGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTTGATTACTGTTGATTTGTTGGCCGTTTTCTTCATTGGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATTCAAAACCGTGCGCGTTTATTAAATCAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATtgt                                                                                              <  1:418651/250‑1 (MQ=255)                                                                                                                                                          aaaaatcG‑ATCCGGAATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCAACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCGATATTGTGGAAg                                                                                         <  2:254892/245‑1 (MQ=255)                                                                                                                                                           aGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTATCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAgg                                                                                        >  1:331902/1‑251 (MQ=255)                                                                                                                                                             aaaCG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGc                                                                                       >  1:370782/1‑250 (MQ=255)                                                                                                                                                                cG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATg                                                                                   >  1:6201/1‑251 (MQ=255)                                                                                                                                                                   aTCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGAt                                                                                                                                                                <  1:154018/172‑1 (MQ=255)                                                                                                                                                                   aTCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGAt                                                                                                                                                                >  2:154018/1‑172 (MQ=255)                                                                                                                                                                    tCCGGGATACACTGCAACATTACAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTCCTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCCGCGCCACCACATCACATATCTCTTTCAGCTCCGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAAAAGTACACAACTCCTCTGAAAAAGCAAGACAGATATATTGGGGAAGGCaaagacg                                                                                >  2:356894/1‑246 (MQ=255)                                                                                                                                                                         gATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTGTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGc                                                                           <  1:201825/251‑1 (MQ=255)                                                                                                                                                                            acacTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCaa                                                                         >  1:232831/1‑250 (MQ=255)                                                                                                                                                                                      aTTAAAGCAAGACCAGCCATTATTGAGCATGTCCCTGCCGTCGATTACATCCTTTGTCTTGTCTGGATTACTGTAGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGGACCAAACTCATCTGAAAAAGCAGCCCAGCTATATTGTGGAAGGCAATGccacaaagaaaagcgagcaac                                                              >  2:294418/1‑232 (MQ=255)                                                                                                                                                                                         aaggcaggACCAGCCATTATTGAGTATGTCATGGCCGTCGATTCCATCCTTTGTATTGGCGGGATTACTGTTGATTTGTTGCCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAGCCGTGCGCGGTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAATCAAAAGGGCGCTACGcc                                                           <  2:220668/244‑1 (MQ=255)                                                                                                                                                                                         aaaGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGcc                                                           <  1:310454/251‑1 (MQ=255)                                                                                                                                                                                         aaaGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGcc                                                           <  1:108813/251‑1 (MQ=255)                                                                                                                                                                                           aGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGACGCTACGcc                                                           >  1:317616/1‑249 (MQ=255)                                                                                                                                                                                                aaCCAGCCATTATGGAGTATGTCCTTGCCGTCGATTCCACCCTTTGTATTGTCGGGATTACTGTTGATTTTTTTGCCTTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCGACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACaa                                                    <  2:445442/250‑1 (MQ=255)                                                                                                                                                                                                     gCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCggg                                               >  2:184319/1‑251 (MQ=255)                                                                                                                                                                                                          tattGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACTCCAGCACAACCGGGAGc                                            >  1:87235/1‑249 (MQ=255)                                                                                                                                                                                                                gTATGTCTTTGCCGTCGATTCCATCCTTTGTAGTGTCGGGTTTACTGGTGATTTGTTTGCCGTTTCCTTCATTTGCCAGCTCCACCACATCACATAGCTCTTTCAGCTTCGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGGAATGCCGAAAGCAAAGGGGCGCTACGCCAGCACAACCGGGAGCAGATaaaa                                    <  1:57814/251‑1 (MQ=255)                                                                                                                                                                                                                               cGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCGGAATgg                     <  1:127397/251‑1 (MQ=255)                                                                                                                                                                                                                                    ccATCTTTTGTTTTGTCTGGATTTCTTTTGATTTGTTGGCCGTTTTCTTCATTTGCCAGCGCCCCCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTc                 <  2:309942/250‑1 (MQ=255)                                                                                                                                                                                                                                               aTTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAACCCGTGCGAGTTTATTAAAACAGAACCAAAATCATCTGAAAAAGCAGGCCAGCTATATTGGGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTCCCcgctccgctc      >  1:378815/1‑250 (MQ=255)                                                                                                                                                                                                                                                   tCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGTAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCTCAAgg  <  1:273920/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        GGGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACG‑ATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCTCAAGG  >  NC_000913/246504‑246994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑