| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,563,778 | (G)6→5 | coding (148/258 nt) | ytiA ← | uncharacterized protein YtiA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,563,778 | 0 | G | . | 96.7% | 124.2 / ‑4.0 | 30 | coding (148/258 nt) | ytiA | uncharacterized protein YtiA |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (14/15); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATT > NC_000913/4563533‑4564022 | cacacTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGt < 2:224913/251‑1 (MQ=255) gCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATAACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGcc > 1:348658/1‑250 (MQ=255) aataatGGCGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTACGGCAAATCAATAAGATATCGAGACGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACACCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑CCAGGTCAGCCAAATGCATAACCCGCAGCcgccgc > 2:252519/1‑251 (MQ=255) tGGAGCTGCGTTTACTTAACGAACTACTTTATGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATAGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAAACCCGTAGTGTGCAATGCAGCGCCAGACACAATCCTGCTGGCAATAAATTGCCTCCGTCAGCCCAGGGATCAACCATTGTATTTACTC‑CCCGGGGGACAGGTCAGCAAGATGCATAACCGGAAGCCGCCGCGTaa > 2:295911/1‑250 (MQ=255) ggAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAAGTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAAt < 2:16622/251‑1 (MQ=255) agaTGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAAACCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATAACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAACTGCATAACCCGAAGACGCCGCGTAAATc > 2:424372/1‑250 (MQ=255) gTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACggggg > 2:80808/1‑250 (MQ=255) aaCGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGc < 1:382327/250‑1 (MQ=255) aGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCATGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTACTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGc > 1:297150/1‑251 (MQ=255) aaaGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGTAATATCGCTGACGCGAAAAGTTATGCCCGCTGCCTGCAGCGCTTTGCGGGttt < 1:291065/250‑1 (MQ=255) atCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGa < 1:80808/251‑1 (MQ=255) gagaAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGc > 2:303066/1‑251 (MQ=255) agaAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCGGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTGCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCTCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGcc < 2:422971/251‑1 (MQ=255) agaAGTATACGTAGCGACCTTAATGAAGCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGGCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAGATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGcc < 1:33436/251‑1 (MQ=255) cGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTggg > 1:438759/1‑250 (MQ=255) aaatacccaTCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCCGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAACAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGGATGACGCCGACCGTGGAGTGaaata > 2:120817/9‑250 (MQ=255) cccTTAACGCGGAGAACCCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGTGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCGGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAAtaaaaataaaaa < 2:273221/250‑1 (MQ=255) cgcgGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAACTTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTc > 1:217590/1‑251 (MQ=255) cAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCaa < 1:238536/251‑1 (MQ=255) gACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTAccgcc < 2:438759/251‑1 (MQ=255) acacCAGCCTGCGGTCAATAAATTGACTGAGTCACCCCAGGGATCACCCATTGAAGTCCCACGCCGGGGG‑AAAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGTAACGTTTTGCGGGTTTGTAGGACGCCGACCGGGGAGTGAAATAAAAATAAAAACTCTTTCATCGTCGTGTCCTCACTCGTTGGCCAATTAccgccg < 2:217590/251‑1 (MQ=255) ctgctgGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAAtt > 1:308234/1‑251 (MQ=255) ggaaaTAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGTCCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCTCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTAGGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTgcg < 1:194290/246‑1 (MQ=255) gCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGGCCTCCCTCGTTGACCAATTACCGCCGCGCCAATTGCGcc > 2:390452/1‑250 (MQ=255) cAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGcc > 1:276417/1‑249 (MQ=255) aTTGACTCAGTCAGCCCAGGGAACACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCCCGCCAATTGCGCCAGCAAAtt > 1:106620/1‑251 (MQ=255) gACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCAGGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCTCTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTgc < 1:452075/250‑1 (MQ=255) agtcagCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCa < 1:303066/251‑1 (MQ=255) gATCCCCCATTGTATGTCCTCGCCGGGGG‑ACAGGTCAGCCAAAGGCATAACCCGCAGCCGCCGCGTAAACCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAAGGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGAGTAttt < 1:120817/251‑1 (MQ=255) ttCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCCCTCGTTGACCAATTACCACCCCGCCAAATGCCCCAGCAAATTGCGCACCCGGATGGGTATTTACCCGCATTCGCAgg > 2:16329/1‑251 (MQ=255) ccgggggACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGGTGGGTATTTACCGGCATTCGCAGGTGAGAtt > 1:80768/3‑251 (MQ=255) | CACACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATT > NC_000913/4563533‑4564022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |