Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,207,549	Δ1 bp	coding (179/606 nt)	ttdB →	L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,207,549	0	G	.	100.0%	260.8 / NA	57	coding (179/606 nt)	ttdB	L(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (23/34);  total (23/34)

CACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGA  >  NC_000913/3207311‑3207789                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 cACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCAGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTgcgc                                                                                                                                                                                                                                     >  1:303587/1‑251 (MQ=255)           gTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGGGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCg                                                                                                                                                                                                                           <  1:373962/251‑1 (MQ=255)                 gCCGATCTCACCGTTGAAAATCTGTCTCACACCCGTAGCGCGTTATGAAAAAACTCCGGACAACCCCCATCAAGTCTGGAGATAGGCAAGACATTCGCGTCGGGGATTTGAGCAACCTGACCGGTACGCTGGTGCCCGGCCGCGACGTTTGGCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTGACGGTCTCAACGGCAAAGCGATTTTCCACGCTG‑TCCCATCGTGCGTAAAAACGGGCACAAAt                                                                                                                                                                                                                     <  2:41166/251‑1 (MQ=255)                               tGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATGCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTTATCTCCCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCGCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGtctc                                                                                                                                                                                                        <  1:47204/250‑1 (MQ=255)                                gAAAATCTGTCTCACCCCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAATCTGAAGATCTGCAAGATATGCGCGTCGGCGATTTGATCTCCCTGCCCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCACCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTcc                                                                                                                                                                                                       <  2:76672/250‑1 (MQ=255)                                gAAAATCTGCCTCACACCCGGAGCGCGTTAGGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGAAAGATATTCGCGGCGGCGATGTGATCTACCTGACCGGTACCCTGGTGTCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGGCCGGTCCCTTACGATCGCAACGGCAAAGCGATGTTCCACGCTG‑CCCCAGCGTGCGCAAAAACGTCGACAAATGGGAGATGGTCTcc                                                                                                                                                                                                       <  2:435730/250‑1 (MQ=255)                                  aaaTCGGTCTCACCCCCGGAGCGTGTTATGAAAAAGACCCTGACACCCCCGACCAAAGCTGAAGACCTGCAAGATATGCGCGTCGGCTATTTGACCGCCCGGCCCGGTACGCTGGTGACCTGCCGCGCCGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGAACTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTc                                                                                                                                                                                                    <  2:111266/251‑1 (MQ=255)                                           cccaagcgggAGCGCGTTATGAAAAAGACTCTAACACCCCCGATCAAAGATGAATATCTGAAAAAAATTCGGGTCCGCGAGGGGATCTACCGGACCGGTACGCGGGTGACCGGCGGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGAAAAATGGGAGATGGTCTCCGTCGGCCCGACa                                                                                                                                                                                           <  2:267308/243‑1 (MQ=255)                                             acccccGGGGCGCGTTATAAAAAAGATCCTGCCAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGGCGGCGATGGGATCTACCTGACCGGTACGCGGGTTCCCTGCCGCGCCGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTGTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAc                                                                                                                                                                                         <  2:125229/248‑1 (MQ=255)                                              cccccggggcggtttATGAAAAAGTCCCGGACAACCCCGACCAAAGATGAAGATCGGCAAGATATTCGCGGCGGCGATGTGACCTACCGGCCCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTGCCACGCTG‑CCCCATCGCGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAcc                                                                                                                                                                                        <  2:296659/238‑1 (MQ=255)                                                 ccGGAGCGCGTTATGAAAAAGATCCGGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATTTGATCTCCCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGt                                                                                                                                                                                     <  1:65443/251‑1 (MQ=255)                                                   ggAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACa                                                                                                                                                                                           >  1:267308/1‑243 (MQ=255)                                                     aGCGCGTTATGAAAAAGATCCTGACAACCCCGCTCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACATGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGACCGACAACCAGTATGc                                                                                                                                                                                 >  2:73070/1‑251 (MQ=255)                                                     aGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTCCCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGtcagg                                                                                                                                                                                 >  2:432029/1‑247 (MQ=255)                                                     aGCGCGTTATGAAAAAGATCCTGACAACCCCGATAAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGAGGTGATCTACCTGACCGGTACGCGGGTGACCTGCCGCGACGTTTGGCACCGCCGTTTGATCGAACGGAAACGGCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGATATGGTCTCCGTCGGCCCGACAACCAGTATGc                                                                                                                                                                                 <  1:448379/251‑1 (MQ=255)                                                        gcgTTATGAAAAATATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTTCCTGACCGGTACGCTGGTGCCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTTCGCAAAAACGGCGACGAATTTGATATTGTCTCCGTCGGCCCGACAACCAGTATGCGTa                                                                                                                                                                              <  1:414697/251‑1 (MQ=255)                                                        gcgTTATGAAAAAGATCCTGACAACCCCGACAAAAGCTGAAGATCTGCAAGATATCCGCGTCGGCGATGTGATCTACCTGCCCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTa                                                                                                                                                                              <  1:325459/251‑1 (MQ=255)                                                            tATGAACAAGATCCTGACAACCCCGATCAAAGCTGACGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCaaa                                                                                                                                                                                                                                  >  2:394340/1‑195 (MQ=255)                                                            tATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCGGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCaaa                                                                                                                                                                                                                                  <  1:394340/195‑1 (MQ=255)                                                             aTGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGa                                                                                                                                                                          >  1:23457/1‑250 (MQ=255)                                                                      acccTGACACCCCCGACCAAAGCTGAAGATCTGCAAGATATTCGGGTCGGCGATGTGATCTACCTGACCGGTACGCGGGTGCCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGaa                                                                                                                                                                <  2:14449/249‑1 (MQ=255)                                                                                ccccgtccAAAGCTGAAGATCTGCAAGATTTTGGGGTGGGCGATGTAATCTGCCTGACGGTTACGCTGGTGACCTGCCGCGACTTTTGTTACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCAACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCTGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTa                                                                                                                                                      <  1:327155/244‑1 (MQ=255)                                                                                 cccGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTAt                                                                                                                                                     >  1:215595/1‑251 (MQ=255)                                                                                           gCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGAcc                                                                                                                                           <  1:296393/251‑1 (MQ=255)                                                                                                   gctgtaagattttatcgttggcaaggtgATCTACCTGACCGGTCCGCTGGTTACCTGCCGCGACGTTTGGCACCGCCGGTTGATCGAGCTTAATCGTCCGTTCACTTACGATCTCAACGGAAAAGCGATTTTCCACGCTG‑CCCCATTGTGCGCATAAACGGCGACAAATGGGTGATGGTCTCCGGCGGCCCGACGACCAATATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGaa                                                                                                                                   <  1:397050/226‑1 (MQ=255)                                                                                                         aGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGaa                                                                                                                                                          >  1:142427/1‑222 (MQ=255)                                                                                                         aGATATTCGCGGCGGCGATGTGATCTACCTGCCCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGaa                                                                                                                                                          <  2:142427/222‑1 (MQ=255)                                                                                                               tCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTgg                                                                                                                       >  2:454632/1‑251 (MQ=255)                                                                                                                   gTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCaaa                                                                                                                   <  1:301896/251‑1 (MQ=255)                                                                                                                      ggCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggt                                                                                                                <  1:409876/251‑1 (MQ=255)                                                                                                                                       gACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGAc                                                                                               >  1:165658/1‑251 (MQ=255)                                                                                                                                           ggTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGCACTGAAACGTCCGATCCCTTACGCTCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGGGGTTGGCAAAGGTGGTATGGGGCCGCTGACCg                                                                                             >  2:372560/1‑249 (MQ=255)                                                                                                                                             tACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCga                                                                                            >  1:231014/1‑248 (MQ=255)                                                                                                                                                          tGCCGCGCCGTTTGTCCCCGCCGTTTGATCGAACTGAACCGTCCGATCCCTTAAGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAACGTTTTGAACGTGACTTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCAGAACGAAGAAGGCTGCCAGaa                                                                             >  2:168977/1‑250 (MQ=255)                                                                                                                                                          tGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTcc                                                                                                                                                                                                       >  1:391172/1‑128 (MQ=255)                                                                                                                                                          tGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTcc                                                                                                                                                                                                       <  2:391172/128‑1 (MQ=255)                                                                                                                                                              gcgACGGTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGAAAAGCGATTTTCCACGCTG‑CCCAATCGTGCGCAAAAACGGCGACAAATGGGTGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGCGGTTGGCAAAGGCGGAATGGGGCCGCAGCCCGAAGGAGGAGGCCAGAAATTcc                                                                        >  2:441600/1‑250 (MQ=255)                                                                                                                                                               cgccggttggccgcggcggttGATGGAACTTAAACGTCCGATCCCGTACGATCGCAACTGCAAATCGATTTTGCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGGATGGAAATTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCaa                                                                       <  2:310815/232‑1 (MQ=255)                                                                                                                                                                       gccACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTAGGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTa                                                                <  2:273067/248‑1 (MQ=255)                                                                                                                                                                              ccgTTTGAACGATCTGAAACGTCCTATCCCTAACGATCGCAACGGCAAAGCGAGTTACCACGCTG‑CCCCTGCGAGCTCAAAAACGGCGACAAATGGGAGATGGTCTCCGGCGGCCCGACAACCAGTATGCGTATGGAGAGTTCTGATCGTGAATTTATTTAGCAGCGCGGCGTGAAACTGGTGGGTGGCAAAGTTGGTATGGGGCCGCTGACCTAAGAAGGATGCCAGAAAGTCAAGGCGCTACATGTGAt                                                        <  2:190953/251‑1 (MQ=255)                                                                                                                                                                               cgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAAGTGAtt                                                       <  1:126591/251‑1 (MQ=255)                                                                                                                                                                                  ttGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGAtttt                                                     <  2:96331/250‑1 (MQ=255)                                                                                                                                                                                    gATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCg                                                                                                                                                                                                      >  2:350465/1‑103 (MQ=255)                                                                                                                                                                                    gATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCg                                                                                                                                                                                                      <  1:350465/103‑1 (MQ=255)                                                                                                                                                                                             gAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCAGCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCt                                          <  2:73557/250‑1 (MQ=255)                                                                                                                                                                                                 cGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGcgcg                                     >  2:327821/1‑251 (MQ=255)                                                                                                                                                                                                     cGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCCCGGTGc                                 >  1:327231/1‑251 (MQ=255)                                                                                                                                                                                                      gATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTg                                  <  2:103803/249‑1 (MQ=255)                                                                                                                                                                                                      gATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTg                                  >  1:103803/1‑249 (MQ=255)                                                                                                                                                                                                         cccTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTgg                              <  2:176372/250‑1 (MQ=255)                                                                                                                                                                                                           cTTACGATCTCACCGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACACCCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGCGCGAAGAGGTCTGCCAGAAATTCACGGCGCTACATGTGATTTGCTCGCCCGACTGCGCGGTGCTggcgg                           >  2:142530/1‑251 (MQ=255)                                                                                                                                                                                                              aCGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCa                         <  1:327821/250‑1 (MQ=255)                                                                                                                                                                                                                   ctcAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAAccc                     >  1:34622/1‑249 (MQ=255)                                                                                                                                                                                                                                  aTTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAACTGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCg                                                                                             >  1:262572/1‑162 (MQ=255)                                                                                                                                                                                                                                  aTTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCg                                                                                             <  2:262572/162‑1 (MQ=255)                                                                                                                                                                                                                                   ttttCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTCTGGGGCCGCTGACCGAAGAAGGCTGCCAGAACTTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGGGATTgg     >  2:3566/1‑248 (MQ=255)                                                                                                                                                                                                                                     ttCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTgaaga  <  1:454632/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 CACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGA  >  NC_000913/3207311‑3207789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑