Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,125,694	Δ1 bp	coding (133/585 nt)	rimJ →	ribosomal‑protein‑S5‑alanine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,125,694	0	T	.	100.0%	120.9 / NA	27	coding (133/585 nt)	rimJ	ribosomal‑protein‑S5‑alanine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (17/10);  total (17/10)

CAGAAAATGGCATTTTGGACTTTTCACAGGGTCTGGTTGCGCAGGTATAGTAATACTTACAGCGTATTAAAGACGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACC  >  NC_000913/1125464‑1125939                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      cAGAAAATGGCATTTTGGACTTTTCACAGGGTCTGGTTGCGCAGGTATAGTAATACTTACAGCGTATTAAAGACGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTTCCAAAAGTGCGCTTAACCACAGACCGACTGTTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGaaa                                                                                                                                                                                                                                  <  1:306189/251‑1 (MQ=255)            aTTTTGGACTTTTCACAGGGTCTGGTTGCGCAGGTATAGTAATACTTACAGCGTATTAAAGACGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTAt                                                                                                                                                                                                                       >  1:236940/1‑251 (MQ=255)                    cTTTTCACAGGGTCTGGTTGCGCAGGTATAGTAATACTTACAGCGTATTAAAGACGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAgg                                                                                                                                                                                                               >  2:154812/1‑251 (MQ=255)                       ttCACAGGGTCTGGTTGCGCAGGTATAGTAATACTTACAGCGTATTAAAGACGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCt                                                                                                                                                                                                             <  2:325158/250‑1 (MQ=255)                           caGGGTCTGGTTGCGCAGGTATAGTAATACTTACAGCGTATTAAAGACGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGcag                                                                                                                                                                                                        <  1:21729/251‑1 (MQ=255)                             gtgTCTGGTTGCGCAGGTATAGTAATACTTACAGCGTATTAAAGACGTTACGGGACGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGAGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCTCGACGAAAGCCACTGTTATCCATCAGGCTGGcaggc                                                                                                                                                                                                      >  2:239011/3‑251 (MQ=255)                                              tataGTAATACTTACAGCGTATTAAAGACGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGAtt                                                                                                                                                                                      >  2:21705/1‑250 (MQ=255)                                                                                         tAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTAtt                                                                                                                                          >  2:388727/1‑251 (MQ=255)                                                                                          aGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTc                                                                                                                                         >  2:300101/1‑251 (MQ=255)                                                                                          aGGTATAGAATGTTTGGCTATCGCAGTAACGCGCCACAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGCTGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTc                                                                                                                                         >  1:28617/1‑251 (MQ=255)                                                                                             tataGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGAc                                                                                                                                      >  2:201813/1‑251 (MQ=255)                                                                                              ataGACTGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGATTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGCGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGAcc                                                                                                                                     >  2:373357/1‑251 (MQ=255)                                                                                                   aTGTTTGGCTATCGCAGTAACGTGTCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGGCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGTCAGGCCAGGCTGGGGATGATTAACGAAGTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTACTCGACCCGGAc                                                                                                                                <  2:311153/251‑1 (MQ=255)                                                                                                   aTGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGAc                                                                                                                                <  1:106128/251‑1 (MQ=255)                                                                                                                gCAGTAACGTGCCAACAGTGCGCTTAACCACAGACCGACTGGTCGAGCGTCTGGTGCATGATCGTGATGCATGGCGGCTTGCGGATTATAACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGGTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGAATAACGACTTTCATAAAAAAGGTACAGCTTTCTAACTTGGCTTATTCGACCCGGACGAAAAAGAGatt                                                                                                                    >  2:59389/1‑250 (MQ=255)                                                                                                                        gTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGCTGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGtt                                                                                                           >  1:178413/1‑251 (MQ=255)                                                                                                                          gCCAAAAGGGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCTGATTATTACGCATAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCTACTGTTATTCATCAGGCTTTCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCATCGTTCGATTTTGGCTGATTCGACCCGGACGAAAAAGAGATTATTGGCGTTg                                                                                                          <  1:231057/250‑1 (MQ=255)                                                                                                                               aaGTGCGCTTAAACACAGACCGCCTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTACTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCCGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAAtt                                                                                                    >  2:436387/1‑251 (MQ=255)                                                                                                                                                                               gTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTAtt                                                    >  2:169432/1‑251 (MQ=255)                                                                                                                                                                                 gATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTc                                                   <  1:388727/250‑1 (MQ=255)                                                                                                                                                                                             cTTGCGGATTATTACGCAGAGAATAGACATTTCCTCAAGACC‑GGGAGCCAGTGCGCGAAGAAAGCCACTGTTATCCATCAGGCTCGCAGGCCCGGCTGGGGATGCTTAACGAATTTCATAAACAAGGTTCAACTTTCTCCTTTGGCTTATTCGACCCGGACGAAATCGAGATTATTGGCGTTGCCCATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTTTTCGATTGGGCaata                                       >  2:270127/1‑248 (MQ=255)                                                                                                                                                                                                 cGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTTGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGc                                  <  1:332156/251‑1 (MQ=255)                                                                                                                                                                                                                agaATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTc                    >  2:445280/1‑250 (MQ=255)                                                                                                                                                                                                                        cATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGa            >  2:161152/1‑250 (MQ=255)                                                                                                                                                                                                                         aTTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCGTTTCATGCCTGCTATCTCGGTTAGGCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAg          <  2:296356/251‑1 (MQ=255)                                                                                                                                                                                                                             ccTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCCGGCTGGGGATGATTAACGAATTTCATAAACAAGGGTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCCAAGGACTCATGTTTGAAGCCCt      >  2:85201/1‑251 (MQ=255)                                                                                                                                                                                                                                 aaGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGAcc  <  1:21705/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CAGAAAATGGCATTTTGGACTTTTCACAGGGTCTGGTTGCGCAGGTATAGTAATACTTACAGCGTATTAAAGACGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACC  >  NC_000913/1125464‑1125939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑