| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,336,411 | 2 bp→AT | coding (83‑84/1260 nt) | murA ← | UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,336,411 | 0 | G | A | 100.0% | 64.6 / NA | 21 | I28N (ATC→AAT) | murA | UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/11); total (10/11) | |||||||||||
| * | NC_000913 | 3,336,412 | 0 | A | T | 95.2% | 60.3 / NA | 21 | I28N (ATC→AAT) | murA | UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (10/10); minor base G (0/1); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
GCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCG‑CCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCG > NC_000913/3336197‑3336623 || gCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGGAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTCCGCACCCAGCTGGCTTATCAGCTTCATTGATGTATCGACGCCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCGGCCAGTAGTGCGGGAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATtgtg < 1:175814/251‑1 (MQ=255) cGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAATGTCGCGGGCATCAATAGGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGa < 1:151971/251‑1 (MQ=255) ggCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGAc > 2:210314/1‑251 (MQ=255) gcCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCCCAGCACCATTCCGTTATACTTTCGCACCCAGATGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTACGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAACTTGTGACTTCGCCCTg > 2:118430/1‑251 (MQ=255) cATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCt > 2:87072/1‑251 (MQ=255) gTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAac > 1:52545/1‑251 (MQ=255) aaCCATATCGAAAGGTGCGCAGAATACATTATCGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTGTCTCACCCAGCTGGCTTAGCATCTTCATTGATGTATCGCCGTCTTTCAGTTTCGGGTCCTTCTGGATCTCTACCTGTTCTTCCGCCAGTAGTGCGTCAAAAAGAGTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTGCGCCCTGGAGCTTCGTTGGCCCCTTAACACGa < 2:136316/250‑1 (MQ=255) aaCCAGATCGCAAGGTGCGCAGAATACATTCACGTCGAGGGCCTCCATATGCACAGAACCCTTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGAGGCAACAAGATTAGGCAGAGCAGCCTTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGa > 2:49263/1‑250 (MQ=255) cAGATAGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATCTGCACAGAAACATTACGTTCTACTTTCGCACCAAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTACGTTTCGGGACGTTCTGGATCTCTACCGGTTATTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAAtt > 1:248541/1‑251 (MQ=255) aCATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGTTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGTAAATTGTGACGTCGCCCTGGGGCTTCGTTGGGCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAg < 1:36200/251‑1 (MQ=255) cgGGCATCAATATGCACAGAATCATTACGTTCTACTTTCGCACCCAGCTTGCTTAGCAGCTTCATTGATGGTTCTACGTCTTGCAGTTTCGGGACTTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTTAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCat < 2:148157/251‑1 (MQ=255) gCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATc > 1:183815/1‑251 (MQ=255) ccttACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGccc < 2:5669/249‑1 (MQ=255) gCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTg < 1:210314/251‑1 (MQ=255) tGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCG‑CCCACTCCg > 2:1873/1‑251 (MQ=255) aGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCG‑CCCACTCCGCAGGGGTATACGCTTTGAt < 2:52545/251‑1 (MQ=255) gggACGTTATGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCG‑CCCCCTCCGCAGGGGTATACGCTTTGATCGacac > 2:265254/1‑251 (MQ=255) gACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTc > 1:247986/1‑103 (MQ=255) gACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTc < 2:247986/103‑1 (MQ=255) tggatctcatcaggTTCTTCCGACAGGAGTGCGGCCAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTCGAGCTTCGTTGGCCCCCGAACAACAAATTTAGCCATTTAGTTTGTTGTTAGTTAACAATTCATATCCGCTACCGGCGAATCGGCCAGATCTCAAAATCCGGTCAGTTTGCGATAGCGCGAGCCA‑TCCGCAGGAGTATCCGCTGTGATCGACGCAGCATGaa < 2:265422/238‑1 (MQ=255) ctACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCG‑CCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCg < 2:41439/247‑1 (MQ=255) || GCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCG‑CCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCG > NC_000913/3336197‑3336623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |