| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 809,217 | 2 bp→AG | coding (40‑41/1290 nt) | bioA ← | adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 809,217 | 0 | C | A | 100.0% | 59.4 / NA | 18 | W14M (TGG→CTG) | bioA | adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (7/11); total (7/11) | |||||||||||
| * | NC_000913 | 809,218 | 0 | A | G | 94.4% | 57.0 / NA | 18 | W14M (TGG→CTG) | bioA | adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (7/10); minor base T (0/1); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAG > NC_000913/808978‑809442 || tGCGGCACAGCTCAATGGCTGGCGCTTGGGTGATCCCGCCAAACATCACATGCGACATGGCATCAATTTGCTACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGAGCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTGCTGCGCTCTCCACCGGATAAACCGGCGGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCg < 2:241809/250‑1 (MQ=255) caattgggggcGCATGGGTGATATCGCCAAACATCACATGCGACAGGGCTTCAATTGGCGACTTCATCGCCGCATTAAGCTGCGTGTGATTGTAGCCGGTGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGGCTGCCGTCAGACAAAATCAGCTCGTAACCATCGGCGCTCGCCACCTGATAAACCGGCAGAGGGGAGGTCATGGATGGGTATGGGCGCAGGATATGGCGTGGGTCAGAGGCa < 2:112839/243‑1 (MQ=255) cAATGGCTTGCGCATGGGTGATCCCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGGGGATCGCCGCCCATCAGGACGACATCCCGTCAACCAGGCGTCTGCCGTCAGACAAAATCATCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGTCAGAGGGGAGGGCATGGATGTGTATGGGGGCAGGATATTTCGTTGGTCAAAGGc < 2:255396/250‑1 (MQ=255) cAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCTCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGc < 1:61907/250‑1 (MQ=255) ggcGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTc > 1:97323/1‑251 (MQ=255) gTGATACCGCCAAACATCACATGCGACATGGCCTCCCTTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGCCATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTAGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGGGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCAt > 1:18290/1‑251 (MQ=255) aTACCGCCAAACATCACAGGCGACATTGCAGCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCATGATATGGCGTTGGGCAAAGTCAAGATCGTCCGTTGTCATAAt < 2:266887/251‑1 (MQ=255) gACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGaa < 1:268855/251‑1 (MQ=255) cATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGaaa > 2:266889/1‑250 (MQ=255) aTGGCATCAATTTGCGACTTCCTCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGaaa > 2:17046/1‑249 (MQ=255) cGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACaa < 1:17046/251‑1 (MQ=255) ccaGGACGACAGTCCGTCAACCAGGCGTCTGTCGTCAGACAAAATCAGCTCTCAACCTGCGGCGCTCTCCACCGGATAAACCGGCAGAGGGGATGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGATAAGCCCCATGGCTCAc < 2:245277/251‑1 (MQ=255) aTACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCt > 1:194003/1‑251 (MQ=255) aGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTAACAGAATTATTTGAAAAAc > 2:210427/1‑251 (MQ=255) aCCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTg < 1:109785/251‑1 (MQ=255) caccacCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGGTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTGGAAAAACCGTTGCTGGATCGGCTGtt < 2:83374/250‑1 (MQ=255) agagGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATc > 1:35957/1‑251 (MQ=255) ggAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAg < 1:145473/251‑1 (MQ=255) || TGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAG > NC_000913/808978‑809442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |