Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,823,162	C→T	H255Y (CAC→TAC)	spoT →	bifunctional (p)ppGpp synthase/hydrolase SpoT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,823,162	0	C	T	100.0%	140.8 / NA	40	H255Y (CAC→TAC)	spoT	bifunctional (p)ppGpp synthase/hydrolase SpoT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/15);  total (25/15)

TTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAA  >  NC_000913/3822915‑3823374                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                     tttAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACt                                                                                                                                                                                                                   >  1:164474/1‑251 (MQ=255)   tAGGTATCCACAACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTACTCAACGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCACATAGCAGCGTTTTTACTCg                                                                                                                                                                                                                 >  2:69277/1‑251 (MQ=255)               ccaTTAAAACCGAACTCGAAGAGCTGGGGTTTGAGGCGTTGTATCCCACCCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGTGAATACCGTGCCGCGGCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATc                                                                                                                                                                                                     <  2:107519/250‑1 (MQ=255)                    aaaaCCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACg                                                                                                                                                                                                 <  1:33064/250‑1 (MQ=255)                                            tttGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAATAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGAtt                                                                                                                                                                         <  1:128055/250‑1 (MQ=255)                                               gAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCGACGCTTTCCGCGTGATCGTCAATGATTCTGa                                                                                                                                                                     <  1:213441/251‑1 (MQ=255)                                                         aTCCCAACCGTTCTCGCGTACTCAAAGAAATGATGAAAGCCGCGCGCGTCAACCGTAAAGAGATGATCCCGAAGATTCTTTCTGCAATCGAAGGGCGTTTGCAGGCAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTAt                                                                                                                                                           >  2:274090/1‑251 (MQ=255)                                                               aCCGTTATCGCGTTCTCAAAGACGTGGTGAAAGCCGAGCGCGGCCACCGTAAAGAGATGATACAGCAGATTCTTTCTGAAAACGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGCGGTCGAGAGAAGCCTCTTTATTCGATTTACTGCCACATGGCGCTCAAAGAGCAGCGTTTTTACTCGATCCTGGTCATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTg                                                                                                                                                     >  2:264595/1‑251 (MQ=255)                                                               aCCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTg                                                                                                                                                     <  1:154211/251‑1 (MQ=255)                                                                  gTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTg                                                                                                                                                  <  1:28320/251‑1 (MQ=255)                                                                                            aacgCCGCGCGCGGCAAACGTAAAGAGATGATCCATAAGAGTCATTCTGAATTCGAAGGGCGTGTGCCGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTGTATTCGATTTACTGCAAAAGGGTGCTCAAAGATCAGCGTTTTTACTCGATCATGGACATCTACGCGTTCAGCGTGCTCGTTGATGTTTCTGACACCTGTTCTCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGcc                                                                                                                        <  2:133277/248‑1 (MQ=255)                                                                                               gCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTGCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCTTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCGGTACAAGCcgcg                                                                                                                     <  1:267898/251‑1 (MQ=255)                                                                                                   cgcgcgGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAACTCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCg                                                                                                                 >  1:91144/1‑251 (MQ=255)                                                                                                       cgGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGcc                                                                                                             >  2:187981/1‑251 (MQ=255)                                                                                                          cAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCcgcg                                                                                                          >  1:56257/1‑251 (MQ=255)                                                                                                                     agaTGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACtat                                                                                               >  1:89441/1‑251 (MQ=255)                                                                                                                         gatCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCg                                                                                           >  1:250771/1‑251 (MQ=255)                                                                                                                                     tctttctGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCaaaa                                                                                <  1:221228/250‑1 (MQ=255)                                                                                                                                     tctttatGAAAGCGAATGGGGTATGCAAGAAGCGGGAACCACGTGCCGCGTCAGTGTGCGCGAGAAGCAACTTTATTCGATTTACTGCAAAATTGTGTTCAAAGAGCAGCGTTTTTACTCTATTATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACTCCTGTTATCGCGGGCTGGGCCATATGAACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATCTCGCCATTCCaaaa                                                                                <  2:232579/250‑1 (MQ=255)                                                                                                                                        tgctGAAATCGAAGGTCGTTTGCAGGAAGCGGGAATTCCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTAGTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACGCTATCATGGTCGTCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTTGGCCAGATGCGTAGGCTGGGCAAGCCGCGTCCGGGCCGCGTGAAAGACTATAGCGCCATTCCAAAAGc                                                                              <  1:231923/247‑1 (MQ=255)                                                                                                                                               aTCGACGGGCGTTTGCAGGACGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGCTTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTACGCGTGATCGTAAATGATTCTGACACCTATTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCCTTCCAAAAGCGAACGGCt                                                                      >  2:97641/1‑250 (MQ=255)                                                                                                                                                  gAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATc                                                                   >  2:103722/1‑250 (MQ=255)                                                                                                                                                   aaGGGCGTTTGCCGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGCGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATc                                                                   >  2:233795/1‑249 (MQ=255)                                                                                                                                                    aGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCCTCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGt                                                                >  1:38825/1‑251 (MQ=255)                                                                                                                                                        cGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCttt                                                            >  1:185237/1‑251 (MQ=255)                                                                                                                                                          gttGCAGGAAGCGGGAACACCGTGCCGCGGCAGTGGTCGCGGGAAGAATCGTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGTTTTCCGCGTGATCGGCAATGATTCTGACACCGGTTATCGCGTGCTGGGCCAGATGCACAGCATGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTAGATCGCCATTCCCAAAGCGAACGGATATCAGTCTTTGc                                                          <  2:16914/250‑1 (MQ=255)                                                                                                                                                              cAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCacac                                                      >  1:77265/1‑251 (MQ=255)                                                                                                                                                               aGGAATCGGGAATACCGTGCCGCGCCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATATACGCTTTCCGCGTGATCGTCAATGATTCTGACACATGTTATCGCGTGCTGGGCCAGATGCACAGCCTGAACAAGCCGCGTCAGGGCCGCGTGAAAGACTCTATCGCCATTCCAAAAGCGAACGGCTATCAATATTTGCACAcc                                                     >  2:141497/1‑251 (MQ=255)                                                                                                                                                                 gAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTc                                                   >  1:49612/1‑251 (MQ=255)                                                                                                                                                                     cGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgatg                                               >  1:93744/1‑251 (MQ=255)                                                                                                                                                                     cGGGAATACCGTGCCGCGTCAGTGGTCCCGAGAAGCATCTTTATTCGATTTCCTGCAAAATGGAGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACCTCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgatg                                               >  2:41308/1‑251 (MQ=255)                                                                                                                                                                         aaTACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCg                                           <  1:13768/251‑1 (MQ=255)                                                                                                                                                                                   cgcgTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACgg                                 >  1:150591/1‑251 (MQ=255)                                                                                                                                                                                                gcgAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTGTCCGCGTGATCGTCAATGATTCTGACCCCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACATCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAg                    <  1:120813/251‑1 (MQ=255)                                                                                                                                                                                                  gagaAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACGCCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGGCCGGGCCGCGTGAAAGACTATATCGCCAGTCTAAAAGCGAGCGGCTATCAGTCTTTTCTCTCCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGt                  <  1:115069/251‑1 (MQ=255)                                                                                                                                                                                                       gCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTcc                >  1:122532/1‑248 (MQ=255)                                                                                                                                                                                                        cATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTCCTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCACTGATTCTGACACGTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAACGACTATCTCGCCATTCCCAAAGCGAACGGATATCAGTCTTTGCCCACCTCGCTGATCGGCCCGCACGGTGTGCCGGTTGAggacc                >  2:277029/1‑244 (MQ=255)                                                                                                                                                                                                            tttATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGAGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCAAGATCCg         >  1:83603/1‑250 (MQ=255)                                                                                                                                                                                                                  cGATTTACTGCAAAATGATGCTCAAAGAGCAGCGTTTTTACTCGATCATTGACATCTACGATTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCTTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAACGAACGGCAATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATcccgaccgac  >  2:118744/1‑243 (MQ=255)                                                                                                                                                                                                                   gATTTACTACAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCACGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGa   >  2:164480/1‑249 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                     TTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAA  >  NC_000913/3822915‑3823374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑