Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,682,515	C→T	M311I (ATG→ATA)	dctA ←	C4 dicarboxylate/orotate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,682,515	0	C	T	100.0%	108.7 / NA	31	M311I (ATG→ATA)	dctA	C4 dicarboxylate/orotate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

TACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAGGATA  >  NC_000913/3682269‑3682758                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    tACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATCAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTCGTGGCCTGGGCGATAAACACCGCCGCTATTg                                                                                                                                                                                                                                                 >  2:255855/1‑251 (MQ=255)      cgttcccgacagggTTAGCCAGCGCACGAGCTTCTGACATAATGCGGTCGATCCCGAGGATCAGCGCCAGCCCCGCTCCCGGCAAATGCCCCCCCGCAGAGAGCGTCGCCGCCATCACAATAAAGCCCCTCCCCGTTCCCCCTGCCGCCCCTTAAGAAGAAAGCAGCAACACGATTAACATCTTGATTTGGTGGACGAGCTCCACCTGATTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAgg                                                                                                                                                                                                                                            <  1:106834/239‑1 (MQ=255)                         cAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGGTATCCATCTGACTGTTAGTGGCCTGGGCGCTAAACCCCGCCGCTATTGTCAGGTATATCGATGTGCCATCa                                                                                                                                                                                                                          >  1:81801/1‑250 (MQ=255)                            cgcACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCAATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGt                                                                                                                                                                                                                      >  1:71075/1‑251 (MQ=255)                               aCGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTa                                                                                                                                                                                                                    >  1:244939/1‑250 (MQ=255)                                                      cGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGCCTGTTAGTGGCCTGGGCGATAAACCCCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATgccc                                                                                                                                                                                            >  2:159610/1‑248 (MQ=255)                                                                         cAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTAc                                                                                                                                                                         >  1:178171/1‑251 (MQ=255)                                                                         cAGACCCGCTACCGGCAAATGGCCCACCGCAGAAAGCGCCGCCGCACGCACGATAAAGCCACTACCCGCTACCCCTGCCGCCCCCTTAGACGCAAGCACCTCCACGATTAACAGCGTGATTTGGAAGACGATATCCATCTGACTGATAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATAGGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTAc                                                                                                                                                                         >  1:126805/1‑251 (MQ=255)                                                                           cccccGCTACCGGCAAATGCCCCCCCGCAGAGAGCGTCGCCGCCAGCACGAAAAACCCACTCCCCGTTCCCCCTCCCGCCCCTTAAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATACCCATCTGACTGTTAGTGGCCGGGGCGAAAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACgg                                                                                                                                                                       <  1:81536/249‑1 (MQ=255)                                                                                      ggCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTCGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGCGttt                                                                                                                                                            >  1:42277/1‑251 (MQ=255)                                                                                                 cACCGCAGAGAGCGTCGCCGCCAGCACGATCAAGCCACTACCCGTTACCCCTGACGCACCTTTAGAAGAAAGCAGCAACAAGATTAACAGCGTGATTTCGTGGACGATATCCATCTGACTGTTAGCGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGCATATCGCTGTGCCCTAAAGGTTAAACGAGTAGCCTGTAGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGt                                                                                                                                                 >  1:125162/1‑251 (MQ=255)                                                                                                     gCAGAGAGCGTCGCCGCCAGCACGATAAAGCCCCTACCCGTTCCCCCTGCCGCCCCTTTAAAAGAAAGCACCAACACGATAAACTGCGTGTTTTGGTGGACGATATCCATCTGACTTTTAGTGGCCAGGGCGAAAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAg                                                                                                                                             <  1:222179/251‑1 (MQ=255)                                                                                                                 cccgccAACACGAAAAACCCACTCCCCTTTCCCCCGCCCCCCCCTTAAGAAAAAAGAAGCAACACGATAAACGGCGTGTTTTGGTGGGCAATACCCATCTGTTTTTTGGGGGCGGGGGCAAAAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGcagcag                                                                                                                                 <  2:205331/250‑1 (MQ=255)                                                                                                                  cggccacaaggATAAAGCCACTACCGTTTCCCCCTGCCGCCCTTTGAGAAAAAAGCAGAAACAGGATTAACAGCGTGATTTGTTGGACGTTTCCCTTTTGATTGTTGGTGGCTTGGGAGAAAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAgc                                                                                                                                <  1:261220/241‑1 (MQ=255)                                                                                                                    gccAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACAAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAgcgc                                                                                                                              >  1:87667/1‑251 (MQ=255)                                                                                                                                            gTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTATCAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTcccc                                                                                                        >  2:181950/1‑249 (MQ=255)                                                                                                                                               aCCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAATATGAAGTCCCCAgaac                                                                                                   >  1:149448/1‑248 (MQ=255)                                                                                                                                                    cgcccccccTTTAAAAGAAAGCAGCAACACTATTAACAGCGTGATTTGGTGGACGATACCCATTTGACTTTTGGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATca                                                                                              <  1:87570/246‑1 (MQ=255)                                                                                                                                                      ccgccCCTTTAAAAGAAGGCAGAAACACGATTAAAAGGGTGATTTGGGGGACGATATCCATCTGATTGTAGGGGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATcagc                                                                                            <  2:277/251‑1 (MQ=255)                                                                                                                                                                               cacGATAAACAGCGTGATTTGGTGGAAGATATCCATCTGACTGTTAGTGGCCTGGCCGAAAACCACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATaa                                                                   <  1:269331/251‑1 (MQ=255)                                                                                                                                                                                          gcttgttttGGGGGACGATACCCATCTGACTTTTAGGGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGACAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATa                                                        <  1:255399/245‑1 (MQ=255)                                                                                                                                                                                                  tgttggACAATACCCTTCTGACGTTTAGTGCCCTGGGCGAAAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAAc                                                 <  1:114679/247‑1 (MQ=255)                                                                                                                                                                                                     tggACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAACCACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTAAAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGt                                             >  2:132815/1‑251 (MQ=255)                                                                                                                                                                                                         cGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCt                                         >  2:4224/1‑251 (MQ=255)                                                                                                                                                                                                                     gattGTTAGTGGCCGGGGCAATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGAtt                                <  2:58290/245‑1 (MQ=255)                                                                                                                                                                                                                         tttAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAAccc                          <  1:5395/249‑1 (MQ=255)                                                                                                                                                                                                                         gTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAAccc                          >  1:184727/1‑250 (MQ=255)                                                                                                                                                                                                                            aGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAAt                       >  1:241973/1‑250 (MQ=255)                                                                                                                                                                                                                                          aaaccacCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAAc        <  1:16643/247‑1 (MQ=255)                                                                                                                                                                                                                                           aaaCACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAAc        >  1:68908/1‑250 (MQ=255)                                                                                                                                                                                                                                                ccgccgCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGACCCCAATACCAGCACCACAAACAGGATa  <  2:115468/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    TACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAGGATA  >  NC_000913/3682269‑3682758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑