Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,612,194	G→A	E110E (GAG→GAA)	yhhT →	putative transporter YhhT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,612,194	0	G	A	100.0%	117.3 / NA	34	E110E (GAG→GAA)	yhhT	putative transporter YhhT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/17);  total (17/17)

GCGGCAGATATCATTGTGCAGCTGTTACTGGCGCTGTTTTTTGCCATCGTCCTCAACCCGCTCGTCACCTGGTTTATTCGTCGGGGAGTACAACGCCCCGTTGCCATTACGATTGTAGTGGTGGTGATGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGAATAATCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGC  >  NC_000913/3611952‑3612440                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       gCGGCAGATATCATTGTGCAGCTGTTACTGGCGCTGTTTTTTGCCATCGTCCTCAACCCGCTCGGCACCTGGTTTATTCGTCGGGGAGTACAACGCCCCGTTGCCATTACGATTGTAGTGGTGGTGATGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTGTTTAAATTGCAGGAAAAGTTGcc                                                                                                                                                                                                                                                <  1:75470/251‑1 (MQ=255)    gCAGATATCATTGTGCAGCTGTTACTGGCGCTGTTTTTTGCCATCGTCCTCAACCCGCTCGTCACCTGGTTTATTCGTCGGGGAGTACAACGCCCCGTTGCCATTACGATTGTAGTGGTGGTGATGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCTGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCttt                                                                                                                                                                                                                                             <  1:153096/251‑1 (MQ=255)                   cAGCTGTTACTGGCGCTGTTTTTTGCCATCGTCCTCAACCAGCTCGTCACCTGGTTCATTCGTCGGGGAGTCCAACGCCCCGTTGCCATTACGATTGTAGTGGAGGTGATGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGACTTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCatat                                                                                                                                                                                                                              >  1:273870/1‑251 (MQ=255)                              ggCGCTGTTTTTTGCCATCGTCCTCAACCCGCTCGTCACCTGGTTTATTCGTCGGGGAGTACAACGCCCCGTTGCCATTACGATTGTAGTGGTGGTGATGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGc                                                                                                                                                                                                                   <  1:149968/251‑1 (MQ=255)                                       ttttGCCATCGTCCTCAACCCGCTCGTCACCTGGTTTATTCGTCGGGGAGTACAACGCCCCGTTGCCATTACGATTGTAGTGGTGGTGATGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAAtgctg                                                                                                                                                                                                           <  1:179713/250‑1 (MQ=255)                                                       aaCCCGCTCGTCACCTGGTTTATGCGTCGGGGAGTACAACGCCCCGTTGCCATTACGATTGTAGTGGTGGTGATGCTGATCGCACTAACCGCGCTGGTCTGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAAGTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGAGTGACTCGGa                                                                                                                                                                                          <  2:153476/251‑1 (MQ=255)                                                               cGTCACCTGGTTTATTCGTCGGGGAGTACAACGCCCCGTTGCCATTACGATTGTAGTGGTGGTGATGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTgg                                                                                                                                                                                  >  2:72528/1‑251 (MQ=255)                                                                  aaCCTGGTTTATTCGTCGGGGAGTACAACGCCCCGTTGCCATTACGATTGTAGTGGTGGTGATGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGtt                                                                                                                                                                                <  2:143918/249‑1 (MQ=255)                                                                   cccTGGTTTATTCGTCGGGGAGTACAACGCCCCGTTGCCATTACGATTGTAGTGGTGGTGATGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTAc                                                                                                                                                                              <  1:213469/250‑1 (MQ=255)                                                                               cgtcgGGGAGTACAACGCCCCGTTGCCATTACGATTGTAGTGGTGGTGATGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGCAAAAGTGGTTACCTTCACCACAgc                                                                                                                                                                  >  1:39559/1‑251 (MQ=255)                                                                                        gtaacaagCCCCGTTGCCATTACGCTTGTAGTGGTGGTGATGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGACGCCGGAGCGAATGATGCAGCGGATGGACTCGGAAAACGTGGTTACCTTcacccacgcgctattgac                                                                                                                                                         >  1:66934/8‑237 (MQ=255)                                                                                            aaCGCCCAATTGCCAGTACGATTGTAGTGGTGATGATGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGCATTTATCACTATGCTGACGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGACTTTTCTTAATTTGCATATGTCGCCGCAGCGGATGCTGCAGAGGATGGACTCGGAAAAAGCGGTTACATTCTCCCCAGCGCTAATGACCggg                                                                                                                                                     >  2:92968/1‑251 (MQ=255)                                                                                                                             tgATGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCAGCATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTg                                                                                                                    >  1:45420/1‑251 (MQ=255)                                                                                                                               aTGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGt                                                                                                                  <  1:72528/251‑1 (MQ=255)                                                                                                                                       cGCACTAACCGAGCTGGTCGGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCCGAGGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTAGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCg                                                                                                          >  2:62278/1‑251 (MQ=255)                                                                                                                                           cTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTTCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGTCTTCCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTa                                                                                                        <  1:89248/249‑1 (MQ=255)                                                                                                                                                             gTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTGTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGt                                                                                    <  2:215880/251‑1 (MQ=255)                                                                                                                                                                  ggcggcATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTgcgc                                                                                <  1:44911/250‑1 (MQ=255)                                                                                                                                                                    cggcATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCAc                                                                             >  1:69021/1‑251 (MQ=255)                                                                                                                                                                               aaCGAATTTATCTCTATGCTGCCGAAGTTGAATAAGGAGCTGACGCGCAAACGTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACGCGGAAAAAGTGGTTAACTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCTCTTACaa                                                                  <  1:191273/251‑1 (MQ=255)                                                                                                                                                                                    aTTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGc                                                             >  1:5811/1‑251 (MQ=255)                                                                                                                                                                                                   tccGAAGGTTAATAAGGAGCGCACGCGCAAACTTTTTAAAGTGCAGGAAATGAGCCCTTTACTTAATTTTCATATGGCTTCGAAGCGAATGCATCAGCGGATGGACGCGGAAAAAGTGGTTTCCTTCACCACTGCGCTAATGACCGGGCATTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTAATGCTGTGCGAAGGGCGCCACGTCCCTTACAAAATTCGTATTGCGCTGaa                                                <  2:245290/248‑1 (MQ=255)                                                                                                                                                                                                   gcCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGaat                                               <  1:278017/250‑1 (MQ=255)                                                                                                                                                                                                    ccGAAGCTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGaata                                              >  1:72958/1‑250 (MQ=255)                                                                                                                                                                                                        aGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGa                                                 >  2:72968/1‑243 (MQ=255)                                                                                                                                                                                                        aGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTACGCTGa                                                 <  1:72968/243‑1 (MQ=255)                                                                                                                                                                                                          tttAATAAGGAGCTGACGCGCACACTTTTTAAACTCCCCGAAATGAAGCCTATTCTTAATTTGCATATGTCGCCGGAGAGATCACTGCAGCGGATGGACTCGGAAACAGAGGTTACCTTCAACACAGCGCTAATGCCCAGGCTTTCCGGGCCAATGGCGAGCGCGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGAATAATCcac                                        >  1:252097/1‑250 (MQ=255)                                                                                                                                                                                                            taataaGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGAATAATCcac                                        >  2:195213/1‑248 (MQ=255)                                                                                                                                                                                                                             gcAAACTTTTTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGAATAATCCACAGATTCACATCGCGGGAtt                     >  1:43660/1‑250 (MQ=255)                                                                                                                                                                                                                             gcAAACTTTGTAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGAATAATCCACAGATTCACATCGCTGGATTa                    <  1:65546/251‑1 (MQ=255)                                                                                                                                                                                                                                   tttttAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGAGATGACCGTAGTTTTTATGCTGTTCGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGAATAATCCACAGATTCACATCGCGGGATTACACcgc              >  1:44111/1‑251 (MQ=255)                                                                                                                                                                                                                                     tttAAATTGCAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGAATAATCCACAGATTCACATCGCGGGATTACACcgcg             <  1:188822/250‑1 (MQ=255)                                                                                                                                                                                                                                              cAGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGAATAATCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAgg   >  2:113997/1‑251 (MQ=255)                                                                                                                                                                                                                                               aGGAAATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGAATAATCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGc  >  1:143045/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       GCGGCAGATATCATTGTGCAGCTGTTACTGGCGCTGTTTTTTGCCATCGTCCTCAACCCGCTCGTCACCTGGTTTATTCGTCGGGGAGTACAACGCCCCGTTGCCATTACGATTGTAGTGGTGGTGATGCTGATCGCACTAACCGCGCTGGTCGGCGTACTGGCGGCATCGTTTAACGAATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGAATAATCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGC  >  NC_000913/3611952‑3612440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑