Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	105.6 / NA	35	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/17);  total (18/17)

AATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAA‑CCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGAC  >  NC_000913/3387796‑3388274                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            aaTGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGACTAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACcgg                                                                                                                                                                                                                                        >  2:177622/1‑251 (MQ=255)        aGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCACTGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGCTGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGcaac                                                                                                                                                                                                                                 >  2:262038/1‑251 (MQ=255)               tataACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATCATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCCACAATcgcc                                                                                                                                                                                                                          >  1:180591/1‑251 (MQ=255)                ataACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATcgcc                                                                                                                                                                                                                          <  1:15396/250‑1 (MQ=255)                       gCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAg                                                                                                                                                                                                                 <  1:37899/251‑1 (MQ=255)                            cccccACGCATACGAGTTTTCTATGCGTACCAGCGAGGATATCAAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAAGGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCg                                                                                                                                                                                                            <  2:102374/251‑1 (MQ=255)                                cACGCATACTATTTTGCTATTCGTAACAGCTAGGATATCCAGTGACAAAAACCGGCCCAGGTACAGCACACCAGAATCATCAATAATGGTGCGCGTATCATCGCAATGATGATCACCAGGCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCTCT‑GTCAGTACCGCCCa                                                                                                                                                                                                        <  2:107430/251‑1 (MQ=255)                                               tCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGGGGCGTTTcc                                                                                                                                                                                           >  2:131570/1‑249 (MQ=255)                                                             cGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGc                                                                                                                                                                           >  1:148808/1‑251 (MQ=255)                                                                 gATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCTCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAgagtgt                                                                                                                                                                       <  2:158812/251‑6 (MQ=255)                                                                 gATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGGGCGCTGATCATCGCAATGATGATCACCAGTCCTTCAAGACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGTTTCACCTCCCTCAGCAAAGGCCGGACCGGCGGCAACAAGCGCTTCTTGT‑AGTGCCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCcaac                                                                                                                                                                       <  1:263658/251‑1 (MQ=255)                                                                  atatCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCcaac                                                                                                                                                                       >  1:192605/1‑250 (MQ=255)                                                                   tatCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCcaaca                                                                                                                                                                      >  1:272350/1‑250 (MQ=255)                                                                                     ggCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAATACGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGAACCAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAACGCCAACAAACAGCGCCAGTACAAt                                                                                                                                                     >  1:109803/1‑249 (MQ=255)                                                                                                 gCACACCAGAATCATCAATAATGGGGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGtt                                                                                                                                       <  1:16177/251‑1 (MQ=255)                                                                                                  cacaCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGttt                                                                                                                                      <  1:60735/251‑1 (MQ=255)                                                                                                          aaTCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGGGGCGTTTCCAGCTGGAAGTGCAAGCCAACAAACAGCGCCAGCACAATGGCGGGCGcccaggttaccgca                                                                                                                               >  2:144739/1‑238 (MQ=255)                                                                                                                  ataagGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCCGCGGGGCGTTTCCAGCTGGAAGTGAAAGCAAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTAACGCAAAACGaaat                                                                                                                      >  1:129046/1‑249 (MQ=255)                                                                                                                   taatGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGt                                                                                                                                                                                                    <  2:75829/172‑1 (MQ=255)                                                                                                                   taatGGCGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGt                                                                                                                                                                                                    >  1:75829/1‑172 (MQ=255)                                                                                                                    aatGGTGCGCGGATCATCGCAATGATGATCACCAGCCCGGCAATACCGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATg                                                                                                                    <  2:194855/251‑1 (MQ=255)                                                                                                                                     cGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTAc                                                                                                                                                        <  1:3288/198‑1 (MQ=255)                                                                                                                                     cGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTAc                                                                                                                                                        >  2:3288/1‑198 (MQ=255)                                                                                                                                               atCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTACCGCAAAGCGAATATGTTGGGTAGCAATGGAGAAAATACCCAt                                                                                         >  1:78442/1‑251 (MQ=255)                                                                                                                                                  cccAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGt                                                                                      <  2:272350/250‑1 (MQ=255)                                                                                                                                                       tCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTTCCCCACAACGAATATTTTGGGTAGCAATGGGGAAAATAACCAtacagattt                                                                                 >  2:40363/1‑243 (MQ=255)                                                                                                                                                          ggCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCTGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGTCGGTCGCCAGTTTTACCGCAAAGGGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAA‑cc                                                                             <  1:17168/251‑1 (MQ=255)                                                                                                                                                                                       tgCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAAAATGTTGGGTAGCAATGGAGAAAATACCCATCGTGATTAA‑CCAAACTCACGCAGGCGATGGGCCATTTTAc                                                >  1:184746/1‑251 (MQ=255)                                                                                                                                                                                           cAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAA‑CCAAACTCACGCAGGCGATGGGCCATTTTACGgaa                                            <  1:147860/251‑1 (MQ=255)                                                                                                                                                                                                        tAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCCCCCGTTTTACCGCAAAACGGAAAAGTTGGTTTGCAATTGAGAAAATACCCATCGTGGTTAAACCACA‑TCCCCCCGGCGATTGGGCATTTTACCGGAGAACGAATCCTgg                               >  2:90160/1‑251 (MQ=255)                                                                                                                                                                                                                 gcgcCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAA‑CCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTtcgtcg                      <  2:110470/251‑1 (MQ=255)                                                                                                                                                                                                                             ggTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAA‑CCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTg          <  2:95164/251‑1 (MQ=255)                                                                                                                                                                                                                               ttCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCCGTTTTACCGCAAAACGAAAATGTTTGTTAGCAATGGAGAAAATACCCCACGTGATTAA‑ACAAACTCACGCAGGCGATGGGCCATTTTTCGGAGGAACGCATCCTGGCTTTCGTCGCGATCTTGttttgc        >  2:253883/1‑248 (MQ=255)                                                                                                                                                                                                                                  aCCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTAACGCCAAGCGAATATGTTGGGTAGCAATGGAGAAAATACCCATCGTGATTAA‑CCAAACTCACGCAGGCGATGGGCCAATTTACGGAAGAACGAATCCTGGCTTTCGGCGCGATCtttgttgcctgt     >  1:89244/1‑241 (MQ=255)                                                                                                                                                                                                                                     ccccGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAA‑CCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGac  <  1:10375/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            AATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCT‑GTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAA‑CCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGAC  >  NC_000913/3387796‑3388274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑