| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,926,292 | C→T | intergenic (+96/‑16) | queF → / → ppnN | 7‑cyano‑7‑deazaguanine reductase/nucleotide 5'‑monophosphate nucleosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,926,292 | 0 | C | T | 95.5% | 66.6 / NA | 22 | intergenic (+96/‑16) | queF/ppnN | 7‑cyano‑7‑deazaguanine reductase/nucleotide 5'‑monophosphate nucleosidase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (12/9); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCC‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGT > NC_000913/2926051‑2926522 | gCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAgg > 1:1395/1‑249 (MQ=255)gCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAGGAg < 1:143812/251‑1 (MQ=255) ttCTGCCAGCCAGACAAATTGAGAGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGGGCAGTAATAGCGATTTTGTACCATCGACCACAACACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTCTTGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAGGAGTTTTTCTTGATTACACATAtt > 2:28294/1‑249 (MQ=255) ccagAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTgg < 1:120988/251‑1 (MQ=255) gTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATAtgtt < 2:1395/251‑1 (MQ=255) tttACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATAtgttg > 1:68103/1‑251 (MQ=255) aCGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTc < 2:105086/250‑1 (MQ=255) gtcgtGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCCTCGACCCCAAGACTGGGTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCTTCT‑CATAAGGAGTTTTTCTTGATTACCCATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAATGGat > 2:155596/1‑251 (MQ=255) ggCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCt > 2:58796/1‑251 (MQ=255) tAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGcc > 1:207345/1‑250 (MQ=255) gCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTg > 2:188616/1‑251 (MQ=255) cccAGCGACCACAAGACTTGTTCGGCAATAAATTTTTGCTCAATTTTGCGGGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAGTAGTTTTTCTTTATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACt < 1:79923/251‑1 (MQ=255) aGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGt < 1:214489/251‑1 (MQ=255) aaactctttCTCAATTTTGCGTGCTGGATTCACGCAGAAGGCTGTGACTGGTCATCAGGCAGGGCTATTGTAATGAAAGGGAATGACGATATTCGCCT‑CATAAGGAGTTTTTCTTGATTACAAATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGATGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGAAACTGTTCACTTGCCGTACTGAACTCCGGTAGt > 2:78469/7‑251 (MQ=255) aaaTTTTTTCGCAAATTAGCGTGCTGGATTCACGCAGAAGGGTGTGAAAGGTCATCAGGCAGGGTTATGGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAGGAGTTTTTCGTATTTACACATATGAGCCCGCTTGGCACCAGGGATAGGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGTGACCTCTATCAACGGTTGCGCAACTGTTCACTGGCCGTACTGAACTCCGGTAg < 2:101270/250‑1 (MQ=255) gTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCaa > 2:75885/1‑248 (MQ=255) tCACGCAGAAGGTTGTGCAAGGTCATCAGGCAGGGCTATTGTAATCAACGGGAATGACGATATTCGTCT‑CATAAGGAGATTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGATTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTACGGCAGTTTGACCGATAACAGCAAAGAATTGCTGtc > 2:8054/1‑251 (MQ=255) ttGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCT‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGaaa > 2:48870/1‑250 (MQ=255) tgtgAAAGGGCATAAGGGAGGGGTATGTTAATCGAAGGGAATGGCGTTATTC‑TCCGCTTAATGAGTTTTTGTTGATTGCTCGTTTAAGCCCGTTTGGGCCCGTGGATATGTTGTGGCGGCTGGAAGTGGATGTGTTTAAACGCCCCGTCATGAGCTAGCTCTGTCAAGTGGTTCGCAACTGGGCACTTGCCGTTCTGAATTCCTGTAGTGTGAGTGATATCAGCAAAGAATTGCTGTCTCGTTTTGaaaa < 1:167855/250‑1 (MQ=255) catcaGGCAGGGCTATTGTAATCAAAGGGAATGACGAGATTCGTCT‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGAGATGTTGTCGCAGCTGGAAGTGGATATGCTGAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTTCCGTACTGAACGCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTa < 2:123160/251‑1 (MQ=255) aaTCAAAGGGAATGACGATATTCGTCT‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAAc < 1:75885/250‑1 (MQ=255) gggAATGACGATATTCGTCT‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCACCTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGt > 2:182214/1‑250 (MQ=255) | GCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCC‑CATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGT > NC_000913/2926051‑2926522 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |