Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,580,388	G→A	P251P (CCG→CCA)	tktB →	transketolase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,580,388	0	G	A	95.5%	61.1 / NA	23	P251P (CCG→CCA)	tktB	transketolase 2
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (11/10);  minor base C (1/0);  total (13/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

GGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATCCATGAAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGGATGCCCGTGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATGAGAAGTTTGCCGCCTATAAAAAGGCTCATCCGCAACTGGCAGAAGAGTTTACCCGACGGAT  >  NC_000913/2580143‑2580618                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       ggcaCGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATCCATGAAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATaa                                                                                                                                                                                                                                   <  1:185683/251‑1 (MQ=255)         gggACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATCCATGAAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATAAAGCAgg                                                                                                                                                                                                                             <  1:15822/249‑1 (MQ=255)            aCTGGGCAAGCTGATTGGTTTTTACGATCACACCGGCCTTTCCATCAACGGTGCAAAAGAAGGCTGGTTTACCGACGACACGACAAAACGTTCTGAAGCCTATCACTGACATGTGATCCATGAAATCGACGGTCACGATCCGCAGGCGGAGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATACGACGTCGCTGATTATCTACCGTACGGTGATTGGCTTTGGTCCGCCAAATAAAGCAGGTAAgg                                                                                                                                                                                                                        >  1:259424/1‑251 (MQ=255)                   aaGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATCCATGAAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATAAAGCAGGTAAGGGAGAggc                                                                                                                                                                                                                 <  1:74573/251‑1 (MQ=255)                             gTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATCCATGAAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATAAAGCAGGTAAGGAAGAggcgcacggcgca                                                                                                                                                                                                       <  2:197587/251‑1 (MQ=255)                                                              gTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATCCATGAAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGGGATTGGCTTTGGGTCGCCAAATAAAGCCGGTAAGGAAGAGGCGACCGGCGCACCACCGGGGGAAGAAGAAGGGGCGCTGGCACgg                                                                                                                                                                      >  2:142592/1‑251 (MQ=255)                                                                     aGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATCCATGAAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATAAATCAGGTAAGGAAGAGGCGCACGGCGCACCACGGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAAc                                                                                                                                                               <  1:254870/251‑1 (MQ=255)                                                                                                     gTTTTGAAGCCTATCACGGGCATGTGATCCATGAAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGAGCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGTTGATTGGCTTTGGATCGCCAAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGACAAAAACTGGGCTGGCGCCATCCGCCATTTGAGATCCCt                                                                                                                               <  1:217755/251‑1 (MQ=255)                                                                                                        ttGAAGCCTATCACTGGCATGTGATCCATGAAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTaa                                                                                                                             >  1:194229/1‑250 (MQ=255)                                                                                                                           tgtgATCCATGAAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGCTCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCCAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTggg                                                                                                         >  2:55027/1‑251 (MQ=255)                                                                                                                              gATCCATGAAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGGATg                                                                                                      <  1:228974/251‑1 (MQ=255)                                                                                                                                           gACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGGGGCGCTGGCACGGCAAAAAATGGGCTGGCACCATCCGCCCTTTGGGATCCCTAAAGGGATTTTTACCGCCGGGGATGCCCGTGAAAAAgg                                                                                         >  1:228005/1‑251 (MQ=255)                                                                                                                                                 cACGATCCGCAGGCGGTGAAGGAAGCGCTCCTTGAAGCGCAAAACGTGCAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCCAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGGGGCGCTGGCACGGCCAAAACTGGGCTGGCAACATCCGCCATTTGAGATCCCTAAAGGGATTTATCACGCCTGGGCTGCCCGTGCAAAAGGCGaaa                                                                                    >  2:20453/1‑250 (MQ=255)                                                                                                                                                    gATCCGCTGGCAGTGGAGGAAGCGCTCCGTGAAGCGCAAAGGGTGAAAGATAAGCCATCGCTGTTGAGCTGCCGTACGGTGATTGGGTTTGGTTCGACAAATAAAGCAGGGTAGGAGGAGGCGCACGGCGCACCTCTGGGGGAAGAAGAAGTGGCGCTTGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGAGCCCTAAAGAGATTTATCACGCCTGGGATCCCCGTGAAAAAGGCGAAAAAgc                                                                                <  2:7500/251‑1 (MQ=255)                                                                                                                                                           aGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGGATGCCCGTGAAAAAGGCGAAAAAGCgcagc                                                                           >  2:188865/1‑249 (MQ=255)                                                                                                                                                                      gaagCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGGATGCCCGTGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATg                                                               >  2:215450/1‑250 (MQ=255)                                                                                                                                                                        agCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGAAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGGATGCCCGTGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATg                                                               >  1:123362/1‑248 (MQ=255)                                                                                                                                                                                       gcAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGGATGCCCGTGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATGAGAAGTTTGCCGCCtata                                             <  1:48737/251‑1 (MQ=255)                                                                                                                                                                                                 aaaGATTAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGGATGCCCGTGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATGAGAAGTTTGCCGCCTATAAAAAGGCTc                                    >  1:71476/1‑250 (MQ=255)                                                                                                                                                                                                                 tGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCAAATAAAGCAGGTAAGGAAGCGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGGATGCCCGTGAAAAAGGCGAAAAAGCGCAGCAGAGCTGTAATGAGAAGTTTGCCGCCTATAAAAAATCTCCTCCGCAACTGACaga                    >  1:275494/1‑250 (MQ=255)                                                                                                                                                                                                                                cGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGGATGCCCGTGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATGAGAAGTTTGCCGCCTATAAAAAGGCTCATCCGCAACTGGCAGAAGAGTTTACCCGACgg    >  2:45368/1‑251 (MQ=255)                                                                                                                                                                                                                                cGGTGATTGGCTTTGGTTCGCCAAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGGATGCCCGTGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATGAGAAGTTTGCCGCCTATAAAAAGGCTCATCCGCAACTGGCAGAAGAGTTTACCCGACgg    >  2:199008/1‑251 (MQ=255)                                                                                                                                                                                                                                  gTGATTGGCTTTGGTTCGCCAAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGGATGCCCGTGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATGAGAAGTTTGCCGCCTATAAAAAGGCTCATCCGCAACTGGCAGAAGAGTTTACCCGACGGAt  <  1:177612/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       GGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATCCATGAAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGGATGCCCGTGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATGAGAAGTTTGCCGCCTATAAAAAGGCTCATCCGCAACTGGCAGAAGAGTTTACCCGACGGAT  >  NC_000913/2580143‑2580618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑