Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,188,087	T→C	T110A (ACG→GCG)	yehA ←	putative fimbrial adhesin YehA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,188,087	0	T	C	100.0%	22.1 / NA	9	T110A (ACG→GCG)	yehA	putative fimbrial adhesin YehA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/4);  total (5/4)

TTTGTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGC  >  NC_000913/2187886‑2188268                                                                                                                                                                                                          |                                                                                                                                                                                      tttGTGTCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAATCCCAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACGGAACCGCCGCCCCTCGCAGAAGTATGCACTTTTGCATTGGTGGCCGCAGGTTTCACCGCAACGGAATAACTACTTGTGAAACTGGTCTGATTATTTGACAACGTGAt                                                                                                                                       <  1:270562/250‑1 (MQ=255)              aTTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTgg                                                                                                                         <  2:211487/251‑1 (MQ=255)                                                   caaCCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATAGTATCGtt                                                                                    >  2:50745/3‑251 (MQ=255)                                                             aacacatgataaaTTGTAATGCGTCACTCCCGCGTATCTATGATGAAGCACTGTATAATGTCGCAACACCATGAATCTTCTCTGAAGCGGCGCCCCCCGCAGAAACATGCACTTTTGCATTACTGGCCGCAGGTTTCACCGCAACTGAATAACCACTGGTGAATCTGGTCTTATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATGCAAAATGTAAATTTAATTTTATGGTATCGTTATTAAgggca                                                                          <  2:39764/241‑5 (MQ=255)                                                                                              gtgtCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGAt                                          >  1:203585/1‑250 (MQ=255)                                                                                                           aGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGACTAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCCTAGGGGTGATAGTATTTGTACtt                             >  2:88734/1‑250 (MQ=255)                                                                                                                  gATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCACCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATg                      >  1:224406/1‑250 (MQ=255)                                                                                                                            cAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCAtt            >  1:47958/1‑250 (MQ=255)                                                                                                                                      aaTCATCCCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGCAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGc  <  1:88734/250‑1 (MQ=255)                                                                                                                                                                                                          |                                                                                                                                                                                      TTTGTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGC  >  NC_000913/2187886‑2188268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑