Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	313,543	G→A	intergenic (‑301/‑1748)	ykgR ← / → insE1	putative membrane protein YkgR/IS3 element protein InsE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	313,543	0	G	A	100.0%	53.6 / NA	16	intergenic (‑301/‑1748)	ykgR/insE1	putative membrane protein YkgR/IS3 element protein InsE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/8);  total (8/8)

GAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGGAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCATAGTTTATCCGGA  >  NC_000913/313300‑313782                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                gAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATAGCGCATCTCATCAGAATGTCGTATCTCCTGGGGTGGTATACAAGATATCGTTGTTGGGGACCTGGGAGAGAAATTGAg                                                                                                                                                                                                                                          <  2:122474/251‑1 (MQ=255)    tttGGCCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTc                                                                                                                                                                                                                                       >  2:43892/1‑251 (MQ=255)     ttggtacaCCAATTTTTTGACATGTATCACCAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCt                                                                                                                                                                                                                                      >  1:210602/8‑251 (MQ=255)                            gtagcacaAATTTGAAGAGATGTATTACATCAACTATCTGTTATTGCACCAACGTCATTGATAAATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACGGGCCCAGTCCGTGCGTTGAGTGGTGCTGAGGAGCATAGCGCATCGCAGCATAATGTCGTATCTCCTGGGGTGTTATTCAAGATATCGTTGTTGGTGACCTGGGAGAGAACTTGAGTGCTATGAAATCGTCAACTATGCCgta                                                                                                                                                                                                               <  2:8928/247‑3 (MQ=255)                                         gAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGAtt                                                                                                                                                                                                  >  1:12754/1‑251 (MQ=255)                                          aaTAGATGTATTACATCAACTATCTTTTATTGCACCAACCTCATTGATATATGTAGCCTGAAGTCAGTTCCGGGAATGAGTCTGCTCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGGTTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGAtt                                                                                                                                                                                                  >  1:161196/1‑250 (MQ=255)                                            tAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTac                                                                                                                                                                                                >  2:119714/1‑250 (MQ=255)                                                                                        atatatGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTTACGGGTTa                                                                                                                                                   <  1:119714/251‑1 (MQ=255)                                                                                                             ttCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGCTTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTtgtg                                                                                                                               >  1:176999/1‑250 (MQ=255)                                                                                                                 gggAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCt                                                                                                                          >  1:71926/1‑251 (MQ=255)                                                                                                                                                       gTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGAGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCgg                                                                                    >  1:11875/1‑251 (MQ=255)                                                                                                                                                                    gaggagCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAATATATCGTTGTTGGTGACCTGGGAGAAAAATTGAGTTCTAtt                                                                                                                                                                                                                                   <  1:276855/95‑1 (MQ=255)                                                                                                                                                                             atCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGAGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTGTACTCTCGGGAGAATAATAGATATTTAATcc                                                              <  2:11875/251‑1 (MQ=255)                                                                                                                                                                             atCGCATCTCATCATAATGGCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGGCCTTGGAGAGAAATTGGGTTCTATTAAACCGGCAGCTAGGCCGGATACATACTGGATTACGCTGCAGGCACGGCTTATGAGAGAACGGGCCGTAGTTACGGGGTAATTTTCTGAAAGAAGTTGGGAGGCTGTATCGGTTAGTCAGTGTGTTGATTGTTTTACTCTCGGGAGAATAATAGATATTTAATcc                                                              <  1:35984/251‑1 (MQ=255)                                                                                                                                                                                                       cccTGGGGTGTTATACAAGATATCGTTGTTGGTGTCCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTGATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTTCTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCttt                                    <  2:242204/250‑1 (MQ=255)                                                                                                                                                                                                                                         aCCTGGGAGAGAAATTGAGTTCTAATAAACCGTCAACTATGTCGGATACATACTGGATTTCACTGCAGGCACGCCTTATGAGAGAACGGGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTTATTGGTTTTCTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAAGTTGACCCCATAGTTAATCCGGa  <  1:79264/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                GAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGGAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCATAGTTTATCCGGA  >  NC_000913/313300‑313782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑