Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,721,136 C→T L38F (CTT→TTT)  ydhI → DUF1656 domain‑containing protein YdhI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,721,1360CT95.0% 62.5 / NA 20L38F (CTT→TTT) ydhIDUF1656 domain‑containing protein YdhI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (14/5);  minor base A (1/0);  total (15/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

AATGTATTGCTGCATTTGGTTACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGCTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTC  >  NC_000913/1720903‑1721360
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                
aaTGTATTGCTGCATTTGGTTACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTg                                                                                                                                                                                                                 >  1:65759/1‑251 (MQ=255)
                    tACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGc                                                                                                                                                                                             >  2:58765/1‑251 (MQ=255)
                                                               cACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACa                                                                                                                                                                                     >  1:234400/1‑216 (MQ=255)
                                                               cACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTGCGGTGCGTCCGTCTACTTGCCTCCGTTTTTCAAAGCATTCGCGTTTGGATGCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACa                                                                                                                                                                                     <  2:234400/216‑1 (MQ=255)
                                                                       gAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTg                                                                                                                                          >  1:213034/1‑251 (MQ=255)
                                                                         atatTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCg                                                                                                                                        <  1:161125/251‑1 (MQ=255)
                                                                         atatTTACTACTCTAACGCAATATATTCGGCCGTAACGGCATCTACTTTGTGAAGTTTCTGCTCAATGCAACAGGATTGACCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCCAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGCCATCTGTCCTCCCTTGTTAATGGATTTATCGTTGTTTGCGATTTGCg                                                                                                                                        >  2:103925/1‑251 (MQ=255)
                                                                            tttattAATCTAACGCAATATATTCGGTCGTATAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCACTTACAAGATCTGGTGTTCGGTGCGTCAGTCTACTTTCCTCAGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTTAAATCTAGCATCCCTTGTTAATGGCTTTATCGCTGTTTGCGATTTGCGttt                                                                                                                                     >  2:233092/1‑251 (MQ=255)
                                                                              tattaATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGc                                                                                                                                   >  1:100724/1‑251 (MQ=255)
                                                                                            aaTATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAAt                                                                                                                     <  2:3686/251‑1 (MQ=255)
                                                                                                tatgcGGTCGAAATGGAATCTACTTTGTGCAGTTTATGCTAAATACCACAGGATTGCCCTTACAAGATCTGGCGTTCGGTGCGTCAGTATACTTTCATACGTTTTTCAAAGCGTTCGTGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCAGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGAATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGATTTTGCCTTGCTCTGGCAATCCTg                                                                                                                 >  2:260380/5‑251 (MQ=255)
                                                                                                             aGGAATCTACTTTGTAAAGTTTATGCGCAGCGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGCCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCCATACTAATTGCGTGGTAAc                                                                                                    >  2:115257/1‑251 (MQ=255)
                                                                                                                                                cAGGATTGACCTTACAAGATCTGGTGTTAGGTGCGTCCGTCTAATTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATATGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTcaaca                                                                  >  2:275427/1‑250 (MQ=255)
                                                                                                                                                               aaGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGAtt                                                                                                              >  1:263933/1‑191 (MQ=255)
                                                                                                                                                               aaGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGAtt                                                                                                              <  2:263933/191‑1 (MQ=255)
                                                                                                                                                                                          actttaatccGTTTTTCAAAGAAATCACGACTGGATTCGTCATCTGGATTGTCGTCCACCGCATGATTCGTCGCTGGATCTACGCCGGTAACATCTGACATCACTTGTTAATGGAATTATCGCAGTTTGCGCTTTGCGTTTGCCTTGCTCTGGCAATACTGCTTGCGTGGTAACTATGTCACTTAAAACAATTACGTATTTCTCAACAATCATTGTAGAGGTAGTTGAGGTTCTTGCCGGATGGTGGCtgt                       >  2:112775/8‑251 (MQ=255)
                                                                                                                                                                                            tttCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTgg                     >  1:12214/1‑251 (MQ=255)
                                                                                                                                                                                                        ttCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATg         >  1:77008/1‑251 (MQ=255)
                                                                                                                                                                                                          cAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACAGCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCa       <  2:77008/251‑1 (MQ=255)
                                                                                                                                                                                                               caatCGCGTTTGGAATCGTCATCTGGTTTGGCGTACACCGCCTGCTTCGTGGCTGGATATACGCCGTTGACATCTTGCATCCCTTGTGAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGAGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACATTCATTGTAGCGGTCGTTGCGGTTCTTGCCGGATGGAGGCTGTGGAATTATTACATGCAAtctc  >  2:71613/4‑251 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                
AATGTATTGCTGCATTTGGTTACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGCTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTC  >  NC_000913/1720903‑1721360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑