Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,711,338	G→A	V171I (GTA→ATA)	rsxE →	SoxR [2Fe‑2S] reducing system protein RsxE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,711,338	0	G	A	94.1%	49.2 / ‑3.7	17	V171I (GTA→ATA)	rsxE	SoxR [2Fe‑2S] reducing system protein RsxE
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (6/10);  total (6/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

ACGCCTTTGGCCTGTATCAATCATTAGGGATTTTTATTCCGCTGATTGTCACTAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAGTATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCAT  >  NC_000913/1711096‑1711579                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  acgcctTTGGCCTGTATCAATCATTAGGGATTTTTATTCCGCTGATTGTCACTAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAATGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTTCTGGGTTCACTACGCGAAATTATCGGCAATGTCACATTGTGTTACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTAcg                                                                                                                                                                                                                                            <  1:33569/250‑1 (MQ=255)       ttGGCCTGTATCAATCATTAGGGATTTTTATTCCGCTGATTGTCACTAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGga                                                                                                                                                                                                                                      <  1:217253/250‑1 (MQ=255)              gTATCAATCATTAGGGATTTTTATTCCGCTGATTGTCACTAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTcc                                                                                                                                                                                                                              <  1:143801/251‑1 (MQ=255)                         tAGGGATTTTTATTCCGCTGATTGTCACTAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAGTATTACGCGTGGAGATTTTCCACACCGACTc                                                                                                                                                                                                                    <  1:50580/250‑1 (MQ=255)                                            gATTGTCACTAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGTCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCCAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGAtgc                                                                                                                                                                                                >  2:63782/1‑251 (MQ=255)                                                      aaCTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGccacca                                                                                                                                                                                        <  1:192180/249‑1 (MQ=255)                                                                           gcTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACt                                                                                                                                                                 >  1:216539/1‑251 (MQ=255)                                                                                        ccgccAAAAAAGGTCCGGCGCTATCGGCACTGGACGGCTTTTAAATTGGTATGGGCGCAACCTGCGCCATGATCGTGCTGGGTTCACTACGCGCAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGATGTTAGGTAGCTGGGCAACAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAgg                                                                                                                                                     >  1:115296/1‑250 (MQ=255)                                                                                                  aGGTCCGGCGCGTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCtga                                                                                                                                          <  2:270850/251‑1 (MQ=255)                                                                                                       cGGCGCTTTCGGCACTGGACGGCTTTTAAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATGATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCGGTTAGGGAGCTTGGCAAAAATAATACGCGTGGAGATTATCCATGCGGACTCCCCTTTCCTGCTGGCGATGCTTCCTCCAGGTGCATTTATTTGCCTGGGACTGATGCTGGCAGGAAAATACCtgattgat                                                                                                                                     <  1:25474/251‑1 (MQ=255)                                                                                                                               tttCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGc                                                                                                              <  1:39058/250‑1 (MQ=255)                                                                                                                                                       gcgcCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTg                                                                                     <  1:233062/251‑1 (MQ=255)                                                                                                                                                       gcgcCATGTTCGGGTTGGGTGCACTACGCGGAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTGATTGGCCTGGGACTGATGCTGGCAGGAAAATCCCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCCGAACGTGCAtt                                                                                      <  2:36420/250‑1 (MQ=255)                                                                                                                                                                                               cAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGATGAATAAAGCaa                                              >  2:183764/1‑250 (MQ=255)                                                                                                                                                                                                       caTTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTgg                                     <  1:78892/251‑1 (MQ=255)                                                                                                                                                                                                                      cAGATGCGCTGTTAGGTAGCTGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAACAGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGcc                      >  2:61100/1‑251 (MQ=255)                                                                                                                                                                                                                                          tGGGCAAAAATATTACGCGTGGAGATTTTCCACACCGAATCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAAAAATCCTCAt  >  1:218694/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  ACGCCTTTGGCCTGTATCAATCATTAGGGATTTTTATTCCGCTGATTGTCACTAACTGTATCGTTGTGGGCCGCGCTGAAGCCTTCGCCGCCAAAAAAGGTCCGGCGCTTTCGGCACTGGACGGCTTTTCAATTGGTATGGGCGCAACCTGCGCCATGTTCGTGCTGGGTTCACTACGCGAAATTATCGGCAATGGCACATTGTTTGACGGTGCAGATGCGCTGTTAGGTAGCTGGGCAAAAGTATTACGCGTGGAGATTTTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCAT  >  NC_000913/1711096‑1711579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑