Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,610,076	T→G	D202A (GAT→GCT)	uxaB ←	tagaturonate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,610,076	0	T	G	100.0%	68.8 / NA	22	D202A (GAT→GCT)	uxaB	tagaturonate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (11/11);  total (11/11)

CACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTG  >  NC_000913/1609835‑1610321                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      cACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCAtt                                                                                                                                                                                                                                              <  1:113269/251‑1 (MQ=255)     ttGCGTTCTTTGTCCGGTTTAATATCGTCAACAAGCAGCACGTTGAGTGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAACCCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCATTGaa                                                                                                                                                                                                                                           <  2:107883/250‑1 (MQ=255)      tGCGTTCTTTGTACGGTTTAATATCGGCAACAATCAGCACGTTGAGCGGATAGTTGTCCAGACGCAGTTCAGTCGCTAAGGAGTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCGCGTCATCGCGTGGATAACCGGTAACGATACGGTCCACTAGCGTAGAATAGAAGCTGTGAGCCTGAGCCAGCCAGTGAAt                                                                                                                                                                                                                                          <  2:130943/250‑1 (MQ=255)            cTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCATTGAATAAATGCt                                                                                                                                                                                                                                   >  2:53654/1‑251 (MQ=255)                gTACGGTTTAATATCGTCAACAATCAGCCCGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAACCAGGTAACAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACACAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTCGCCTGAGCCAGCCATTGAATAAATGCTTCTg                                                                                                                                                                                                                               >  2:43567/1‑251 (MQ=255)                  aCGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCATTGAATAAATGCTTCTgg                                                                                                                                                                                                                              <  1:117096/250‑1 (MQ=255)                            atCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCAct                                                                                                                                                                                                                   >  1:8947/1‑251 (MQ=255)                                                tGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCAGAGCGCAGCAc                                                                                                                                                                                                <  1:202903/250‑1 (MQ=255)                                                        tatTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACg                                                                                                                                                                                       >  1:28555/1‑251 (MQ=255)                                                                          tCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTGCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGt                                                                                                                                                                      <  2:210081/250‑1 (MQ=255)                                                                                                                gTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTATTCCCGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCCTTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTc                                                                                                                                                                              >  1:247283/1‑204 (MQ=255)                                                                                                                gTAAAAGTGTTCAGCGGTGTCGAGAAAACCAGCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGGTAGCCTGAGCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCCCGCAACGCGTc                                                                                                                                                                              <  2:247283/204‑1 (MQ=255)                                                                                                                            aGCGGTGTCGAGAAAACCATCGTGCTAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGt                                                                                                                   >  2:131246/1‑251 (MQ=255)                                                                                                                            aGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTACTTCCAGTTCAGCCACTTCCTCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACCGAAGCTGTTAGCCTCCGCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGATCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGt                                                                                                                   >  1:156379/1‑251 (MQ=255)                                                                                                                                                      agcccAGTTCTTCTTCCAGTTTAGCCACTACAACGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCATGGAATAAAGGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCCTAATCCAGCGCACCGTTGAAATGGCTAAAGCGTTTGAAcagc                                                                                         <  2:73132/249‑1 (MQ=255)                                                                                                                                                      aaCCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATATCCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGAGCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAAcagc                                                                                         <  2:214506/251‑1 (MQ=255)                                                                                                                                                                                        gcgcgGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTg                                                       <  1:171396/251‑1 (MQ=255)                                                                                                                                                                                          gcgGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGgc                                                     >  1:192901/1‑251 (MQ=255)                                                                                                                                                                                                aaCCGGTAACGATACGGTCCACCAGCGTAGAACAGTCGCTGTTAGCCTGAGCAGGCCATTGAATAAAAGCTTCTGGCAGTGCCCACACTTGAGCATAGAACAGCAACCGTTCACGCAACGCGTCGCCATTCTAGTCAATCAACTCACACGGCATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGCACCGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTc                                                >  1:34002/1‑250 (MQ=255)                                                                                                                                                                                                  ccGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGAGCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATc                                             >  2:138555/1‑251 (MQ=255)                                                                                                                                                                                                                            aGAACAGAAGCTGTTAGCCTGAGCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTg                    <  1:261279/250‑1 (MQ=255)                                                                                                                                                                                                                                             ccTGAGCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGgtggtg  >  1:115587/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      CACTTTGCGTTCTTTGTACGGTTTAATATCGTCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTG  >  NC_000913/1609835‑1610321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑