Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,400,978	C→T	G266S (GGT→AGT)	abgT ←	p‑aminobenzoyl glutamate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,400,978	0	C	T	100.0%	110.7 / NA	33	G266S (GGT→AGT)	abgT	p‑aminobenzoyl glutamate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/21);  total (12/21)

AATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACCTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAACGCAGCTGCCGCTTCCGTGCTGATCCCCGACAGCAACACGTCG  >  NC_000913/1400737‑1401213                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            aaTCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTCAATCCGCCTGCCGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAACGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACAATCAGCGCAATCGCCGCAATAAAAAGTAGCGATACGACACTTGCTATGCg                                                                                                                                                                                                                                    >  2:84468/1‑251 (MQ=255)              ctTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCaaa                                                                                                                                                                                                                           <  1:240519/247‑1 (MQ=255)                      ggTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTc                                                                                                                                                                                                               <  1:60121/251‑1 (MQ=255)                       gTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGTAATCGCAGCAATAAAAAGGAGCGATACGACACTTGCTATTCGTAAACCAAAACGCTGACTTTCg                                                                                                                                                                                                              <  2:273635/251‑1 (MQ=255)                                      gCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCGGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCTCGTTTTTCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTACGGTCAATGACTGCAg                                                                                                                                                                                                <  2:68494/250‑1 (MQ=255)                                                                           gATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGGGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCATCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAACCGCTGACTTTCGGTCAATGGCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAGCCGTg                                                                                                                                                          <  2:224513/251‑1 (MQ=255)                                                                               ccATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTc                                                                                                                                                      <  2:215749/251‑1 (MQ=255)                                                                                                aacaaAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCACTGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGttt                                                                                                                                     >  2:51596/1‑250 (MQ=255)                                                                                                      gAAAAAAAGAATGAACAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCTCGCAATATCCCGTTGTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAATCGCTGACTTTCGTTCAAGGTCTGCAGTTTCTCAGCGCTTTTGCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGgc                                                                                                                               <  1:168712/251‑1 (MQ=255)                                                                                                                          gCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCcaac                                                                                                                        >  1:32270/1‑238 (MQ=255)                                                                                                                          gCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCcaac                                                                                                                        <  2:32270/238‑1 (MQ=255)                                                                                                                              gATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAAGCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATTTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTa                                                                                                       <  1:226594/251‑1 (MQ=255)                                                                                                                               aTACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTAc                                                                                                      <  1:234115/251‑1 (MQ=255)                                                                                                                                 aCCTTTAATAAAGGGTGATGGCAGCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAATCGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACg                                                                                                     <  2:276779/250‑1 (MQ=255)                                                                                                                                  ccTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACgg                                                                                                    >  1:271779/1‑250 (MQ=255)                                                                                                                                        ataaAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGc                                                                                             <  1:51596/251‑1 (MQ=255)                                                                                                                                        ataaAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTGAGCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGc                                                                                             <  1:230484/251‑1 (MQ=255)                                                                                                                                          aaaGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTTGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGGCAATGTCGGCAGTGTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGAGTTTGTCTGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGcc                                                                                            <  1:84468/250‑1 (MQ=255)                                                                                                                                            aGGGTGAGGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGGCACTTGCTATGCTTAATCCAAAACGCTGACTTTCGTGCAATGTCTGCAGTTGCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTGATCAGGCCGCCAGCAATCGTCAGTACGACGACTGAGCTGGCCATa                                                                                         <  2:269947/251‑1 (MQ=255)                                                                                                                                            aGGGTCATGGAATCACGGTGTGATTAATCGCCTCGCGCACTATCCCGTTTTGCAGGATCACCATCAGCGCACTAGCAGCAATAAAAAGTAGCGATACGACAATTGCTATGCGTAAAACAAAACGCTGACTTTCGGTCACTGTATGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACCATAGTCAGCACGACTACGGAGCTGGcaat                                                                                          >  2:36335/1‑247 (MQ=255)                                                                                                                                             gggTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATaa                                                                                        <  1:204400/251‑1 (MQ=255)                                                                                                                                              ggTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTGCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATaaa                                                                                       <  2:184593/251‑1 (MQ=255)                                                                                                                                                        tCACGGTGCGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGtt                                                                              >  1:69732/1‑250 (MQ=255)                                                                                                                                                                     aaTCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGTTTATCAGGCCGCCAACAATCGTCAGTACGTCTACGGAGCTGGCCATAAAATACCATTTATCAATTACACTg                                                                 <  1:159667/250‑1 (MQ=255)                                                                                                                                                                        cGGATCTCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAATCGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATGCCAGTTATCAATTACACTGACg                                                              <  1:102087/250‑1 (MQ=255)                                                                                                                                                                        cGGATCGCGCAATATCCCGTTGTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACGACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGt                                                             <  1:38808/251‑1 (MQ=255)                                                                                                                                                                                             ttGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCGATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTGGCGGATTGAACGCa                                         <  1:44247/250‑1 (MQ=255)                                                                                                                                                                                                    aTCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGACAGTACGACTACGGAGCTGGCAATAAAATACCAGTTATCAATTACACTGACGTGCCTTTGCGGATTGAACGCAGCTGCCGc                                 >  2:201062/1‑251 (MQ=255)                                                                                                                                                                                                             aGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAACGCAGCTGCCGCTTCCGTGCt                        >  2:183586/1‑251 (MQ=255)                                                                                                                                                                                                                        agcaATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAACGCAGCTGCCGCTTCCGTGCTGATCCCCGAc              >  1:166237/1‑250 (MQ=255)                                                                                                                                                                                                                               aaaaGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCAATTGCGGATTGAACGCAGCTGCCGCTTCCGTGCTGATCCCCGACAGCAacac      >  1:218261/1‑251 (MQ=255)                                                                                                                                                                                                                                aaaGTCGCGATACGACACTTGCTCCGCGTCAACCAAACCGCCGACATTCGGAAAATGTATGCAGCTTCTCACCGCCCTTTCACTGCCATTGACCTCAACGTGGCTCGATGATTTTGCCGGTTATCAGGCCGCCAACACTCGTCCGTACGACTACGGAGCTGGCCATACAATACCCGTTATCAATTACCATGACGTGCATTTGCGGATTGAACGCAGCTGCCGCTTCCGTGCTGATCCCCGAAAGCAACACg     >  2:13960/1‑251 (MQ=255)                                                                                                                                                                                                                                   gTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAACGCAGCTGCCGCTTCCGTGCTGATCCCCGACAGCAACACGTCg  >  2:46539/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            AATCCCGCCATCTCTTTCATCGGTTCAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACCTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTTCCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAACGCAGCTGCCGCTTCCGTGCTGATCCCCGACAGCAACACGTCG  >  NC_000913/1400737‑1401213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑