Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,357,202	C→T	intergenic (‑92/+221)	sapA ← / ← ymjA	putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,357,202	0	C	T	100.0%	58.5 / NA	17	intergenic (‑92/+221)	sapA/ymjA	putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/7);  total (10/7)

TTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCC  >  NC_000913/1356977‑1357435                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          ttGACTTGCCCGCTGAAGCAATCGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTCAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTg                                                                                                                                                                                                                   >  2:225386/1‑250 (MQ=255)               gACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGt                                                                                                                                                                                                     <  1:216316/250‑1 (MQ=255)                  gCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCa                                                                                                                                                                                                  >  2:2360/1‑250 (MQ=255)                       aGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTc                                                                                                                                                                                            <  2:249621/251‑1 (MQ=255)                          cAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTa                                                                                                                                                                                         <  1:2360/251‑1 (MQ=255)                                                                                                     cAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGACATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGAGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAAccc                                                                                                               >  1:109573/1‑250 (MQ=255)                                                                                                                   gaCGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGc                                                                                                <  1:166788/251‑1 (MQ=255)                                                                                                                              cTGGCGCATAATATTAAGGGATTTTATGTAAAGCAGCTATCTTACTAATATTTAATGACATTTGCCCTTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTc                                                                                      >  1:252719/1‑250 (MQ=255)                                                                                                                                  cgcATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGt                                                                                 <  1:238208/251‑1 (MQ=255)                                                                                                                                            tAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATg                                                                       >  1:175181/1‑251 (MQ=255)                                                                                                                                               gggATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATgcgg                                                                    >  1:240383/1‑251 (MQ=255)                                                                                                                                                             agagGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTATCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGAGGCGTGAACGCTTTAt                                                      >  2:4841/1‑251 (MQ=255)                                                                                                                                                              gagtcTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATc                                                     <  1:72396/247‑1 (MQ=255)                                                                                                                                                                        tACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACa                                               <  1:25776/247‑1 (MQ=255)                                                                                                                                                                                            tGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTTAACAGGTGACGTCGCCATCTGTTAAACATTCGTACCCGTGATTTCTCTATACATATAACTCACAGAACACCTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACACATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGCTACTGACCGGTCGTCTGCa                        >  1:123769/1‑250 (MQ=255)                                                                                                                                                                                            tGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCa                        >  2:69456/1‑250 (MQ=255)                                                                                                                                                                                                                 ggggTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGCCGCCATCTGTTCAACATTCGTCCACGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGTGGAGCAGAGCGGGCCGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGACAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGCCCGTTCGACTGCAATTAAATCATTACTCATTAccc  >  1:278735/1‑251 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          TTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCC  >  NC_000913/1356977‑1357435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑