| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,322,325 | G→A | L208F (CTC→TTC) | trpE ← | anthranilate synthase subunit TrpE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,322,325 | 0 | G | A | 92.3% | 26.0 / ‑5.6 | 13 | L208F (CTC→TTC) | trpE | anthranilate synthase subunit TrpE |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (5/7); minor base C (1/0); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
CCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTT‑CGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAA > NC_000913/1322112‑1322547 | ccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGATTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGATGACGTAGTT‑CGTTCAGGCGAGCAGTGACACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTg > 1:45056/1‑251 (MQ=255) cAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGAGGCGCGGCTTCGGTCAGTTGCTGACGTAGTT‑CGTTCCGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCTTTCGGAGCAAACAGGCTgg > 2:69462/1‑251 (MQ=255) gttGACGGGCAGGGCAGAGAGAAACGTCTAGATCGCACCACCTGGAAAATTTCTCCAGCGCGAAGCGCTTTTTGCAACAAACGCACTACGCCACCTAACTCATCATCGCTCTGATTACAGTCACAACGCATATGCGGCACGGAAACCTCTGGCAGCGGCGGCGCGGCTTCTGTCAGTTGCTGACGTAGTT‑CGTTCAGGGGATCAGTGAAACGTTGTTTTTCTTCTCCATTCGGAGCAAACAGGCTGGccgg < 2:194323/249‑3 (MQ=255) caccacCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTT‑CGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATCCGGGTGCTTTTTTTCTGATGGTCAATCAcc > 1:178019/1‑250 (MQ=255) ccAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAGCTCTTCATCGCTCTGATTACATTCACATCGCATATGCGGCACGGAAACCGCTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTG‑CGTTCAGGCGTGCGGTGAAACTTTGTTTTTCTTCTGCATGCTGAGCAAACAGGCTGGCCTGAATACGGGTGCTGTTTTGCTGATGTTGAATGACCATCAGCGTTTTATCGAGATa < 2:133187/251‑1 (MQ=255) cGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTT‑CGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGaaa < 1:59975/250‑1 (MQ=255) ttttGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTTCATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTT‑CGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCGTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTTCTTTTTTTCTGATGGTCAATCGCCAGCAGCGTTGCAGCGAGATGAAAACAGAAATCAg < 1:85841/250‑1 (MQ=255) cTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTT‑CGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCt > 2:210174/1‑251 (MQ=255) ccTGGCAGGGGCGGCGCGGTTTGGTTCGGTTGCTGTCGTGTTT‑CGTGCAGGGGGGCGTTAAAACGTTGTTTTTTTTCTTCATTCGGAACAACCGGGCGGGCCTGAATAGGGGTTTTTTTTTTCGTAGGGTCAATCACCATCAGCGTTCCAGGAAAAAAAAAACAAAAACCGGGGCCGTTATTTTCCGCTGAAAGTTGGGGAAAAACTAAAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCgaagaa < 2:84199/250‑1 (MQ=255) ggcggtTTCGTTCAGTTGCTGGCGT‑GTTGCGTACAGGCGAGAAGTGAAACGTTTTTTTTCTTCTTCATGCGGAGAAAACAGGGTGGCTTGAATGCGGGTGTTTTTTTTCGGATGGTCAACCACCATCAGCGTTTCAGGGAAAAAAAAACAAAAATCGGGGCATTTTTTTCCCGCTGACATTGGGGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGtt < 1:1971/250‑1 (MQ=255) gTAGTT‑CGTTCAGGCGAGCAGTGAAACGTTGTTTTGCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAAGACGGGTGCTGTTTTTCTGATGGTCATGCACGATCAGCGTTTCAGCGAGATAAAAGCAGAAATCAGTTCAGTTATTTTCCGCTGACAGTTGCGGTaaa < 1:117014/167‑1 (MQ=255) gTAGTT‑AGTTCAGGAGAGAAGACAAACGTTGTTTTTCTTCTTCATTGGGAGCAAAAAGGCTGGCCTGCATAAGCGTGATTTTTTTATGATGGTAAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGCCCGTTGCGGaaaa > 2:117014/1‑167 (MQ=255) cGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCac > 2:30597/1‑232 (MQ=255) | CCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTT‑CGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAA > NC_000913/1322112‑1322547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |