Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 246,747 G→T S12S (TCG→TCT)  yafL → NlpC/P60 family protein YafL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913246,7470GT96.3% 81.5 / ‑4.1 27S12S (TCG→TCT) yafLNlpC/P60 family protein YafL
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (18/8);  total (18/9)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

ATTGGGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATG  >  NC_000913/246501‑246974
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   
attagtgTATCTCCTGTGCTGTATATTTATTCAGCTTGCATTGTAGCTCAAATGAGCAAAAGCGACAATCCATGAGTAATTTAATAAACGTATGTTTTTACATAATACACTGACATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGAt                                                                                                                                                                                                                                 >  2:180171/7‑251 (MQ=255)
   ggggTATCTCCTGAGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTAc                                                                                                                                                                                                                              >  2:182629/1‑251 (MQ=255)
           tcCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGAtt                                                                                                                                                                                                                      >  1:122789/1‑251 (MQ=255)
             cTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGCCAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCATTTGTATTGTCTGGATTACTGTTGAtttg                                                                                                                                                                                                                    >  1:96178/1‑251 (MQ=255)
                           tattCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTttctt                                                                                                                                                                                                      >  1:246287/1‑251 (MQ=255)
                                                      aGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACcccatcacat                                                                                                                                                                            >  2:152918/1‑250 (MQ=255)
                                                            aGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTCTTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCCCATATCTCtt                                                                                                                                                                     >  2:87798/1‑251 (MQ=255)
                                                                               tttAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCcgcccgcc                                                                                                                                                   >  1:48747/1‑250 (MQ=255)
                                                                                        gggATGTTTTAAAATAATTAACTGAAATATATATGTTAATTTGTGAATAATGTTTTATTAAGTTTGTCCAGAAACGATCCGGGATACACTGCAACATAAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCa                                                                                                                                         <  2:53796/250‑1 (MQ=255)
                                                                                                  tACATAATTAACTTAAATATATATTTTAATTTTATAATAAGGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCTTGACCAGCCATTATTGAGTTTTTCCTTGCCGTCGATTCCTTCCTTTGTATTGTCTGGATTACTGTTTATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATAGCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTgc                                                                                                                                <  1:182629/250‑1 (MQ=255)
                                                                                                          tAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTttatt                                                                                                                       >  2:104708/1‑251 (MQ=255)
                                                                                                             cTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTAAGAAACGATCCGGGATACACTGCAACATTAATGAATGTCCTGCAATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGTATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCCGCGGATGCAAAACCGTACGCGTttcttac                                                                                                                     >  2:204290/1‑249 (MQ=255)
                                                                                                                  atataaatGTAATTTTTATAATAATGTTTTATTAAGTTTGTTAAAAAACGATCCGGGATCCACTGCAACATTAAAGCATGCCCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTAAGCAACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGt                                                                                                               <  2:175712/251‑1 (MQ=255)
                                                                                                                                   ataataATGGTTTATTAAGTTGGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCTTCGATTCCATCCTTTGTATTGTCTGGATTACTGTGGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaa                                                                                               <  1:189465/250‑1 (MQ=255)
                                                                                                                                        aatGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGc                                                                                          <  1:224131/250‑1 (MQ=255)
                                                                                                                                                          ttCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCTAACTCa                                                                                                     >  1:129465/1‑221 (MQ=255)
                                                                                                                                                                 aCGATACGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCCGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAAt                                                                >  1:233144/1‑251 (MQ=255)
                                                                                                                                                                          gATACACTGAAAAATTAAGGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTTGATTCCATCCTTTGTATTGTCTGGATTACTTTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCTCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGTAAGGCAATGCCGAAAGc                                                       <  1:150782/251‑1 (MQ=255)
                                                                                                                                                                               acTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAg                                                  <  1:104708/251‑1 (MQ=255)
                                                                                                                                                                                 tGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGACGGCAATGCAGAAAGCAAAAggg                                                >  2:88243/1‑251 (MQ=255)
                                                                                                                                                                                       aTTAAAGCATGCCCAGCCTTTATGTAGTATTTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTTCCAGCGCCCCCACATCACATAGCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCATGCCAGCTATATTGTTGAAGGCAATGCCGaaaagatcggaagagcgt                                          <  1:21428/251‑16 (MQ=255)
                                                                                                                                                                                            aGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGcc                                       >  2:166943/1‑249 (MQ=255)
                                                                                                                                                                                                   ccAGCCATTCTTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGAAAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGAGCGCGTTTATTAAAACAGTACCAAACTCATCTGCAAAAGCAGGCCAGATATATTGTGGACGGCAATGCCGAAAGCAAAAGGGCGCTAAGCCAGcacaccc                              >  2:121299/1‑248 (MQ=255)
                                                                                                                                                                                                   ccAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAAcc                              >  1:225898/1‑251 (MQ=255)
                                                                                                                                                                                                                  tATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAAc               >  2:218640/1‑251 (MQ=255)
                                                                                                                                                                                                                              gTCGATTCCATCCTTTGTATTGTCTGGCTTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACAACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAACCCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATcccaaat   >  2:151330/1‑246 (MQ=255)
                                                                                                                                                                                                                                  aTTCCCTCCTTTTTTTTGTCTGGATTACTTTTGATTTGTTTGCCTTTTTCTTCATGTGCCAGCGCCACCACATCCCAGATCTCTTTCAGCTCCGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACGCATCTGAAAAAGCAGTCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGTTGGAGATAAAACAGCATCCAGAATg  <  1:88884/248‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   
ATTGGGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATG  >  NC_000913/246501‑246974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑