Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,992	A→T	N271Y (AAT→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,992	0	A	T	100.0%	57.6 / NA	21	N271Y (AAT→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (9/12);  total (9/12)

CCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCA  >  NC_000913/1301768‑1302234                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    ccGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTc                                                                                                                                                                                                                          >  1:25125/1‑251 (MQ=255)             aaTTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGaaa                                                                                                                                                                                                              <  1:215478/251‑1 (MQ=255)                             gACCCAGCCTGGTAATATCGTCACTAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACg                                                                                                                                                                                              <  2:123406/251‑1 (MQ=255)                                                  caccacCGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                                                                                                         >  2:86405/1‑251 (MQ=255)                                                       aCGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACAGGACGTATAACAGCGTGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGc                                                                                                                                                                    <  1:36886/251‑1 (MQ=255)                                                                         aagataaggTCGTAAACGAACGAATAGTAATTGAACGCAGCCCGAACTACTGGAACCACGCGAAACCCGTTATTAACCAGATCACCTATTAGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATGGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCAAGTTGATCCATACCTGTGCACTGACTATTACGaactt                                                                                                                                                  >  2:123053/8‑248 (MQ=255)                                                                               gggTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaacaa                                                                                                                                             <  1:203161/250‑1 (MQ=255)                                                                                         cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGc                                                                                                                                  <  1:212328/251‑1 (MQ=255)                                                                                                         gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcg                                                                                                                  <  1:64212/251‑1 (MQ=255)                                                                                                                               acaacGCGAAAACCGTAATTAACAAGGAAACATATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACAGTAGGGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCAAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGGGCACTTACTATTACGAAATTAAAAAACAGAAACCGCCATTCAACGATGAGCGTGTGCGTACCGCACTGaaaat                                                                                             >  2:150762/1‑248 (MQ=255)                                                                                                                                    gcgAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTAc                                                                                                                                                        >  2:40804/1‑186 (MQ=255)                                                                                                                                    gcgAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGAGCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTAc                                                                                                                                                        <  1:40804/186‑1 (MQ=255)                                                                                                                                           ccGTTATTAACCAGGTATCCTATTTGCCTATTGCTTCTGAAGTTACCGATTTCAACCGCTACCGTAGTGGGGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACTAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTTCCGCACTGAAACTAGGTATGTACcgc                                                                                <  1:90311/251‑1 (MQ=255)                                                                                                                                                               tATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGt                                                            >  1:163822/1‑251 (MQ=255)                                                                                                                                                                        aTTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAgcgc                                                    >  1:268237/1‑250 (MQ=255)                                                                                                                                                                             ttCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAg                                                                                                                                         <  2:22318/160‑1 (MQ=255)                                                                                                                                                                             ttCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAg                                                                                                                                         >  1:22318/1‑160 (MQ=255)                                                                                                                                                                                     ttACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGccc                                      >  1:49461/1‑251 (MQ=255)                                                                                                                                                                                               aacccccacccggaGTGGTAAAACCGAAATGACGTAAAACAGCAGGCCGATCGAATT‑GTCCCAGAAGCTAAAAAAAGAGACCCCGGACGAAGTTCACGTTGATCCATCCCTGTGCCCTTACTATTACAAAATAAACAACAAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGtt                            <  2:49461/238‑1 (MQ=255)                                                                                                                                                                                                   cgctccgttattgtgaaaaTCAACATGACTTATAACAGAATGCC‑AACGCATTTGTTCCACAAGCTGAAAAAAGAGACCCGGGACGAGGTTCACGTTGTTCCATTCCGTTACACTTACTATTACAAAATTACCACCAAGAAACCGCCATTCATCTATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGCCCGCGATATCTTTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTacac                        <  1:152538/235‑1 (MQ=255)                                                                                                                                                                                                                         aCATGACGTATAACAACTTGCCGATCAAATT‑GTCCCAAAAGCTGAAAAAAGAGATCCCGGACAAAGTTCACGTTGATCCATCCCTGTGCATTTACTATGACGAAATAAACAACCAAAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATGGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCa  <  1:229919/251‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    CCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATT‑GTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCA  >  NC_000913/1301768‑1302234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑