Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,298,624	C→T	P9P (CCC→CCT)	ychE →	MarC family putative inner membrane protein YchE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,298,624	0	C	T	100.0%	12.8 / NA	6	P9P (CCC→CCT)	ychE	MarC family putative inner membrane protein YchE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/3);  total (3/3)

AGCTAACAGTTAATAAATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGC  >  NC_000913/1298404‑1298761                                                                                                                                                                                                                             |                                                                                                                                          aGCTAACAGTTAATAAATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGTCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGTCTCTTGCTTCGGAGCTAACCGTGATTCATACCGTTTTTGATTGGCCTGTTTACTTCAAATGTTTCATCGGGTTAtt                                                                                                              <  2:154754/250‑1 (MQ=255)                                ggtggCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCg                                                                              <  1:181745/251‑1 (MQ=255)                                                                                  aGCAATTTTACGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCACCCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGCAACAAAACt                            >  2:45622/1‑251 (MQ=255)                                                                                    cAATTTTCAGCAGTTAGGCGGTGACAAATGCTGTAAAAAGGCGTATTGTCAGCACGTCTTTTAAACCATATTTATGGCTAACATTCTCCGGCTTTTGCTTCGGAGCTAACCGTGATTCATACCTTTTTTGACTTTCCTGTTTACTTCAAATTTTCCATCGGGTTATTTGAGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATAAGCATGACCAGTTATCAGCCAGCGGCAGCGCGAAACAAAACTa                           >  2:177475/1‑250 (MQ=255)                                                                                                     gaggTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACctgt         >  2:252142/1‑251 (MQ=255)                                                                                                            aacaTGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCGTATTTATTGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCGTTTTTGATTTTCCTGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTAACAGCATGACCAGTTATCATACAGCGGCAGCGCGGATCAAAACTAACCTTACGGCTACTCTGTCTGTGGc  <  2:250640/248‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                          AGCTAACAGTTAATAAATTGTAGTATGATTTGGTGGCTACATTAGCATGTTTTGCACAACTAGATAACAATAACGAATGATAGCAATTTTAAGTAGTTAGGAGGTGAAAAATGCTGTCAAAAGGCGTATTGTCAGCGCGTCTTTTCAACCTTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGC  >  NC_000913/1298404‑1298761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑