Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,295,985	C→T	A713T (GCG→ACG)	adhE ←	fused acetaldehyde‑CoA dehydrogenase and iron‑dependent alcohol dehydrogenasealdehyde/alcohol dehydrogenase AdhE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,295,985	0	C	T	100.0%	86.2 / NA	25	A713T (GCG→ACG)	adhE	fused acetaldehyde‑CoA dehydrogenase and iron‑dependent alcohol dehydrogenasealdehyde/alcohol dehydrogenase AdhE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/16);  total (9/16)

GCCCGGTGCGCTCAGACCCAAGTGGTCGGCAATTTCAGCATAACGACGGCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGG‑TAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACACAGGGACTTCGGCATGTCCATAACCAGGTTGG  >  NC_000913/1295758‑1296215                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        gcccggggcgcgcagcgccgaGTGGTCGGCAATTTCAGCATAACGGCGGCGGGCCTGCGGTCGGTCATACTGGCTGAATGCAGTCTGCGTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGCAGCTGGGAACCCAGTTTGGGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATGCCCGCGATAGTCGCTGCAc                                                                                                                                                                                                                  <  2:179805/231‑1 (MQ=255) gCCCGGTGCGCTCAGACCCAAGTGGGCGGCAATTTCAGCATAACGACGGCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACAGACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCAc                                                                                                                                                                                                                  <  2:108785/251‑1 (MQ=255)   ccGGTGCGCTCAGACCCAAGTGGTCGGCAATTTCAGCATAACGACGGCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACtg                                                                                                                                                                                                                >  2:147138/1‑251 (MQ=255)          gcTCAGACCCAAGTGGTCGGCAATTTCAGCATAACGACGGCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAacac                                                                                                                                                                                                         <  1:264179/251‑1 (MQ=255)                                       cATAACGACGGCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCt                                                                                                                                                                            >  2:134265/1‑251 (MQ=255)                                        aTAACGACGGCGGGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCtt                                                                                                                                                                           <  2:273968/251‑1 (MQ=255)                                          aaCGACGGCGAGCCTGCGGACGGTCATACTGGCCGAATGCAATCTCCTTGATCCGGATGTCGTTCGCATTGTAGCGAATAACGTTACAAATCACCAGGGCGTTTGCCAGACCGTGCGTAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATCCCCACGCTAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCg                                                                                                                                                                         >  1:118592/1‑251 (MQ=255)                                                ggCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGACCCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGG‑t                                                                                                                                                                    >  2:92373/1‑249 (MQ=255)                                                 gCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGTGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGG‑TAgg                                                                                                                                                                 >  2:63399/1‑251 (MQ=255)                                                 gCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCACACGTGATACCCGCGATAGTCGCTGCACTGTGAACCCGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGG‑TAgg                                                                                                                                                                 >  2:161237/1‑251 (MQ=255)                                                         gCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGG‑TAGGACGCTg                                                                                                                                                           <  1:161237/249‑1 (MQ=255)                                                                  cATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACGCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGG‑TAGGACGCTGGCAGATATTc                                                                                                                                                 <  1:7934/250‑1 (MQ=255)                                                                  cATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGT‑TATGACGCTGGCAGATATTCt                                                                                                                                                <  2:116884/251‑1 (MQ=255)                                                                             aaTGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGG‑TAGGACGCTGGCAGATATTCTTTCAGCAGttt                                                                                                                                     <  1:169231/251‑1 (MQ=255)                                                                                                 gTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGG‑TAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGAc                                                                                                                 <  1:92373/251‑1 (MQ=255)                                                                                                          cgcattacaGCGAATAACGGTACAAATCAGCAGGTCGTTTACCAGAACGTGCGTAATATGGAACTAGGAACCAAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAAGAGTACGAAAAAATTAAACGCGCGAAAGTCGCGGCACTGTGAACACGTTCACGCGCTACCGTATTTTTATACCCTTC‑TGGCTAGTAGGCTGGCATATATTCTTATAGCAGTTTCAGTGCCCGCCGATCCTGACCATCag                                                                                                           >  1:4462/9‑248 (MQ=255)                                                                                                                               aCAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGG‑TAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGaaa                                                                                   <  2:3570/251‑1 (MQ=255)                                                                                                                               aCAAATCAGCAGGGCGGTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCGTCGTGG‑TAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGaa                                                                                    <  2:154510/250‑1 (MQ=255)                                                                                                                                         agGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTGAGACCCTTCGTGG‑TAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTc                                                                         <  1:112421/251‑1 (MQ=255)                                                                                                                                                                           gggAACCCAGTTTGTGCGCCATTGAGTGACATACACCAAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGCCCCTTCGTGG‑TAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGCGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGCGTTACTGCGTCCAGACCACCGaa                                        >  2:109152/1‑250 (MQ=255)                                                                                                                                                                                              cATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGG‑TAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACACAGGGACTTCGGCa                    <  1:147138/251‑1 (MQ=255)                                                                                                                                                                                                 tGAGTGACATACACCACGGCAGGCGTTCCCAAACGTGATCACCGCGATAGTAGCTCCACTGTGAACACGCTCCCGAGCTACCGGCTATTTAGACCCTTCGAGG‑CAGGACGCTGGCAGATGTTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGCCCATCAGAGCACTCAGCTGCCAGTACAGAAACATAAGCTTCAATGGCGTACGTTACTGCGTACAGAACAACGAAAGCACACAGGGACGTCGCCATGt                 >  2:153217/1‑251 (MQ=255)                                                                                                                                                                                                              cccAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGG‑TAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACACAGGGACTTCGGCATGTCCATAACCAGGtt    <  1:59729/251‑1 (MQ=255)                                                                                                                                                                                                               ccAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGG‑TAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACACAGGGACTTCGGCATGTCCATAACCAGGTTg   <  1:98429/251‑1 (MQ=255)                                                                                                                                                                                                                cAGGAAGGCGTTCGCAAACGTGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGG‑TAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACACAGGGACTTCGGCATGTCCATAACCAGGTTgg  <  1:109152/251‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        GCCCGGTGCGCTCAGACCCAAGTGGTCGGCAATTTCAGCATAACGACGGCGAGCCTGCGGACGGTCATACTGGCTGAATGCAGTCTGCTTGGTCGGGTTGTCGTTCGCATTGTAGCGAATAACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGG‑TAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTCCAGACCACCGAAAGCACACAGGGACTTCGGCATGTCCATAACCAGGTTGG  >  NC_000913/1295758‑1296215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑