Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,323	C→T	noncoding (6/85 nt)	tyrV ←	tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,323	0	C	T	100.0%	19.2 / NA	9	noncoding (6/85 nt)	tyrV	tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/6);  total (3/6)

CAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTC  >  NC_000913/1287088‑1287498                                                                                                                                                                                                                                            |                                                                                                                                                                                cagctcggcgcgcTTCGCCCTGCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGTAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTaa                                                                                                                                                                    <  2:220868/242‑1 (MQ=21) cAGCGCTGCGCGCTTCGCCCTGCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAAtt                                                                                                                                                                  <  2:191588/251‑1 (MQ=255)                       cGGGTCGTTTCCTGCGGCAAGGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTTCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTg                                                                                                                                             <  2:193639/250‑1 (MQ=255)                                      ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGCAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGAAATTCAAATTTTGAGGTAATGCTTGAGAtgggggtgggggg                                                                                                                             >  1:116393/1‑250 (MQ=255)                                                                                                           gAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTAATCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCt                                                        <  1:247020/251‑1 (MQ=255)                                                                                                                 ttAATTGCGGATTCGTTGGGAAGTTCTGGGACTTTTGAACGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATTACGGCAGATTTAAAGCATGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAAATTTTGTGGTACTGCTTGTGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCtcgaat                                                   <  2:61894/250‑1 (MQ=38)                                                                                                                              cGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGGGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCTAACCTtcgtcg                                      >  1:211758/1‑250 (MQ=255)                                                                                                                                            gggACTTTTGAAAGTGATGGAGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCCGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAACTTTTGAGGAAATGCTTGCGATGGCGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCACCGTTCTCTCGCTTTCGCTCGACTCGCACCTTAGTCGAAGGTTCTCACCCtt                       >  2:97928/1‑251 (MQ=37)                                                                                                                                                                 ggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATGTAAAGTCTGCTCCGTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCTCTCCTCACCCGTCGTGTCGTGGCCTGTGGCAACGTGCTCTCGCTTTCGCTCGAATCGAACCTGAGTCGAAGGTTCTCACCTTTCCCCGATGAGTGCAAACTTTc  <  1:97928/251‑1 (MQ=37)                                                                                                                                                                                                                                            |                                                                                                                                                                                CAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTC  >  NC_000913/1287088‑1287498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑