Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,294	C→A	noncoding (35/85 nt)	tyrV ←	tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,294	0	C	A	100.0%	29.4 / NA	11	noncoding (35/85 nt)	tyrV	tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (4/7);  total (4/7)

TTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTC  >  NC_000913/1287055‑1287498                                                                                                                                                                                                                                                |                                                                                                                                                                                                             tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTcc                                                                                                                                                                                                    <  1:204176/250‑1 (MQ=255)        gTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGGGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGcc                                                                                                                                                                                            >  1:117442/1‑251 (MQ=255)                                  cagctcggcgcgcTTCGCCCTGCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGTAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTaa                                                                                                                                                                    <  2:220868/242‑1 (MQ=21)                                  cAGCGCTGCGCGCTTCGCCCTGCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAAtt                                                                                                                                                                  <  2:191588/251‑1 (MQ=255)                                                        cGGGTCGTTTCCTGCGGCAAGGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTTCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTg                                                                                                                                             <  2:193639/250‑1 (MQ=255)                                                                       ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGCAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGAAATTCAAATTTTGAGGTAATGCTTGAGAtgggggtgggggg                                                                                                                             >  1:116393/1‑250 (MQ=255)                                                                                                                                            gAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTAATCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCt                                                        <  1:247020/251‑1 (MQ=255)                                                                                                                                                  ttAATTGCGGATTCGTTGGGAAGTTCTGGGACTTTTGAACGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATTACGGCAGATTTAAAGCATGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAAATTTTGTGGTACTGCTTGTGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCtcgaat                                                   <  2:61894/250‑1 (MQ=38)                                                                                                                                                               cGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGGGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCTAACCTtcgtcg                                      >  1:211758/1‑250 (MQ=255)                                                                                                                                                                             gggACTTTTGAAAGTGATGGAGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCCGATTTAAAGTCTGCTCCCTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAACTTTTGAGGAAATGCTTGCGATGGCGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCACCGTTCTCTCGCTTTCGCTCGACTCGCACCTTAGTCGAAGGTTCTCACCCtt                       >  2:97928/1‑251 (MQ=37)                                                                                                                                                                                                  ggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATGTAAAGTCTGCTCCGTTTGGCCGCTCGGGAACTCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCTCTCCTCACCCGTCGTGTCGTGGCCTGTGGCAACGTGCTCTCGCTTTCGCTCGAATCGAACCTGAGTCGAAGGTTCTCACCTTTCCCCGATGAGTGCAAACTTTc  <  1:97928/251‑1 (MQ=37)                                                                                                                                                                                                                                                |                                                                                                                                                                                                             TTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTC  >  NC_000913/1287055‑1287498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑