Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,272,743	C→T	C79C (TGC→TGT)	chaC →	glutathione‑specific gamma‑glutamylcyclotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,272,743	0	C	T	100.0%	34.6 / NA	15	C79C (TGC→TGT)	chaC	glutathione‑specific gamma‑glutamylcyclotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/10);  total (5/10)

TTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGT  >  NC_000913/1272522‑1272963                                                                                                                                                                                                                              |                                                                                                                                                                                                                             ttCTTGCTGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGGACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGAATgc                                                                                                                                                                                                 >  2:175278/1‑251 (MQ=255)     tgatgaATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTgcg                                                                                                                                                                                                <  2:245101/248‑1 (MQ=255)               cGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGTACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGAc                                                                                                                                                                                   <  2:264164/251‑1 (MQ=255)                                     gCCATTGAAGAAGCACTCTTATGGTCAGAAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTGGATGTGGAATCCGGCTCTGGAGTTTACCGAATTGTGCACCGGTACACTGGTGGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAgg                                                                                                                                                              <  2:201669/250‑1 (MQ=255)                                                                             cggcTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCagaag                                                                                                                       >  1:19696/1‑249 (MQ=255)                                                                                                   ggCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAAc                                                                                                 <  2:143310/249‑1 (MQ=255)                                                                                                                            gtgtGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTAc                                                                      <  1:169525/251‑1 (MQ=255)                                                                                                                                ggATCTACGGCTATGGATCGTTGATGTGAAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCCCAGGCGTCGCCTATCGACTGCCAGAAGAGCCGCTGGAGCAGGCACTAACCCTGTTGTGGCAGCGAGAGATGAGTACCGGc                                                                  >  2:67398/1‑251 (MQ=255)                                                                                                                                                         gtgGAATCCGGCCCTGGAGTTTACCGAAGCGTGCACCGGTACACTGGTTGGATGGCATCGCGAATTTTTTCTGCGCCTGCCCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAATGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTTCCGGCTGTTATCTGCCAACTTGGTGTCAg                                          <  2:129410/250‑1 (MQ=255)                                                                                                                                                           ggAATCCGGCACGGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGctt                                       <  1:175278/251‑1 (MQ=255)                                                                                                                                                                     ccTGGAGTTTCCCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCAGTTTGTCTGCGCCTGACCGCCGGGCGCGGAACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGAGCTtgatg                             <  2:167884/250‑1 (MQ=255)                                                                                                                                                                           gtgtACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGAc                       <  2:10465/248‑1 (MQ=255)                                                                                                                                                                                ccGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGcaca                  <  2:65283/251‑1 (MQ=255)                                                                                                                                                                                              ggTACACTGGTTGGATGGCATCGCGCACTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATtgt    >  2:75977/1‑251 (MQ=255)                                                                                                                                                                                                tACACTGGTTGGATGGCATCGCGCATTTTGTCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTgtgt  >  1:175068/1‑251 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                             TTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCCAGAAGAGACGCTGGAGCAGGAACTAACCCTGTTGTGGAAGCGAGAGATGATTACCGGCTGTTATCTGCCAACCTGGTGTCAGCTTGATCTTGATGATGGACGCACAGTAAACGCCATTGTGT  >  NC_000913/1272522‑1272963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑