Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,265,018	C→T	P3S (CCT→TCT)	prfA →	peptide chain release factor RF1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,265,018	0	C	T	100.0%	35.3 / NA	11	P3S (CCT→TCT)	prfA	peptide chain release factor RF1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/3);  total (8/3)

AACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATG  >  NC_000913/1264889‑1265252                                                                                                                                  |                                                                                                                                                                                                                                           aaCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGTCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGCCCAGGAACGATTTCGCGCATTATCACGCGCATATgc                                                                                                                   >  2:81083/1‑251 (MQ=255)                           gTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGTCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGTTTCGCGCTGtt                                                                                         >  2:272458/1‑251 (MQ=255)                                            cgCCTGCATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCATATGAAGTCTTCTATCGTTGCCAAACTgg                                                                                                                                                                                                                       >  1:166291/1‑108 (MQ=255)                                                           cgcgACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGTCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGATCAGGAAGATATc                                                         >  2:161723/1‑251 (MQ=255)                                                                  gCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACATGGTGCATTTACGCCTATGAAGTCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGGTGGGTGACGCGCAAACTATCGCCGATCAGTATCGTTTTCGCGCATTATCACGCGAAGATGCGCAGTTAAGTGATGTTTCTGTCTTGTTGACCGGCTGGCAACAGGTTCAGGAAGATATCGAAACCg                                                  <  1:265999/251‑1 (MQ=255)                                                                                 aGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGTCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCg                                   >  1:3577/1‑251 (MQ=255)                                                                                 aGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGTCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCg                                   >  1:43093/1‑251 (MQ=255)                                                                                  gCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGTCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCg                                   >  1:114436/1‑250 (MQ=255)                                                                                         atcatTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGTCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCt                           <  1:76687/251‑1 (MQ=255)                                                                                                      tttACAGGGTGCATTTACGCCTATGAAGTCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTgag               <  1:240993/250‑1 (MQ=255)                                                                                                                   tttACGCCTATGAAGTCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATg  >  2:215620/1‑250 (MQ=255)                                                                                                                                  |                                                                                                                                                                                                                                           AACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCGACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATG  >  NC_000913/1264889‑1265252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑