Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	109,960	G→A	R561H (CGT→CAT)	secA →	protein translocation ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	109,960	0	G	A	95.2%	55.4 / NA	21	R561H (CGT→CAT)	secA	protein translocation ATPase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (10/10);  total (11/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCATGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGATTGCCAACG  >  NC_000913/109720‑110166                                                                                                                                                                                                                                                  |                                                                                                                                                                                                               ccAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCTCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCTCGATGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCATCACGAATcc                                                                                                                                                                                                      <  1:176585/251‑1 (MQ=255)     aTTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTAGCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCATCACGAATCCcgtc                                                                                                                                                                                                  <  1:133816/251‑1 (MQ=255)                aCGAAGCGGCGAATGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGGCCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCATCACGAATCCCGTCGTATCGATAAc                                                                                                                                                                                       <  1:42194/251‑1 (MQ=255)                              gttgCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTc                                                                                                                                                                          <  1:33299/250‑1 (MQ=255)                                        caggTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGGACAGCTATTTTGCTCGGTGGGAGCTGGCAGGGCGAAGTTTCCGCGCTGGGAAAACCCACCGCCGAGCAAATTTAAAAAATTAAAAACGCCTGGCAAGGAAGGCACGATGCGGGGATGGGAGGCGGGGGCGCGCATATCATTCGGT‑CCCCGCGACACCAATCCCCTCCTATCGATAACCAGCTGCGCgggcgctcttggcgt                                                                                                                                                               >  1:188517/1‑238 (MQ=255)                                                                                      gggTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATg                                                                                                                 <  1:157579/250‑1 (MQ=255)                                                                                                                gCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCCACTTGAAAAAATTAAAGCCGACTGGCAGGTCCGTCCCGATGGGGGACTGGAAGCAAGTGGCCTGCATATAAT‑CGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGGTCTGGTCGTACGGGGGGTGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGGTGCGTCTTTTTGTTTcctgc                                                                                       >  2:230862/1‑248 (MQ=255)                                                                                                                        gcagAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATc                                                                               <  1:111067/251‑1 (MQ=255)                                                                                                                             aGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCa                                                                          <  1:232409/251‑1 (MQ=255)                                                                                                                                     cgcTGGAAAATCCGACCGCGGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGCACGTCACGAAGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGGTGGAAGATGCGCTGATGCGGATTTTTGCTTCCGACCGAGTTTACGGCATGATGCgg                                                                  >  2:239774/1‑250 (MQ=255)                                                                                                                                                ccGACCGCAGAGCAAATTGAAAAAAGTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGGATTTTTGCTTCCGACCGAGTATCCGGCATGATGCGTAAACTGGGTAt                                                       <  2:137534/251‑1 (MQ=255)                                                                                                                                                        agagCAAATTGAAACAATTAAAGCCGACTGGCAGGTAAGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCATGATGCGTAAACTGGGTATGAAGcc                                                 >  1:225055/1‑249 (MQ=255)                                                                                                                                                                  gAAAAAATTAAAGCCGACTGGCAGGGACGTCACGATGCGGTACTGGAAACAGGTGGCCTGCATATCAT‑CGGTACCGAGCATCACGAATCCCGGCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGCTGGAAGATGCGCTGGTGCGTATTTTTGCTTCCGACCGAGTATCCGGCATGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCAt                                     >  2:58229/1‑251 (MQ=255)                                                                                                                                                                      aaaTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCATGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGa                                  >  1:46227/1‑250 (MQ=255)                                                                                                                                                                           aaaGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCACATCAA‑CGGTACCGCGCATCACGAATCCCGTCGTCTCGATAACCAGTTGCGCGCTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGCTGCGCTGATGCGTATTTTTGCTTCCGACCGCGTATCCGGCATGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACAccc                            >  1:174191/1‑251 (MQ=255)                                                                                                                                                                             aGCCGACTGGCAGGTACGTCACGATGCGGTACTGGCAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCATGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGt                          >  1:137045/1‑251 (MQ=255)                                                                                                                                                                                 gacgggCAGGTACGTCCCGATGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCATCACGAAGCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCATGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGt                      <  2:143465/247‑1 (MQ=255)                                                                                                                                                                                          gTACGTCACGATGCGGTACTGGACGCAGGTGGCCTGCCTATCCT‑CGGCACAGAGGATAACGCATCCCGTCGTATCGATCACAAGTTGCGCGGTCGTTCTGGTCGTCCGGGGGATGCTGGCTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGCGTATCCGGCATGACGCGTAAACTGGGTCTGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGc             >  2:250699/1‑251 (MQ=255)                                                                                                                                                                                                     tGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGGACCGAGCATCACGAATCCCGTCGAATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGATCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTACGACCGAGTATACGGCATGATGCGTAAAATGGGTATGAAGCCAGGAGAAGACATTGAACACCCGTGGGTGACTAAAGCGATTGCCAACg  >  1:61839/1‑251 (MQ=255)                                                                                                                                                                                                                               atatCAT‑CGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTGTGCGTCCGACCGAGTATCCGGCATGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGc             <  1:238966/214‑1 (MQ=255)                                                                                                                                                                                                                               atatCAT‑CGGCACCGCGCATCAAGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCATGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGc             >  2:238966/1‑214 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                               CCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCAT‑CGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCATGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGATTGCCAACG  >  NC_000913/109720‑110166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑