Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,040,126	G→T	L277L (CTG→CTT)	appB →	cytochrome bd‑II subunit 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,040,126	0	G	T	100.0%	58.3 / NA	18	L277L (CTG→CTT)	appB	cytochrome bd‑II subunit 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/10);  total (8/10)

ATTCATCTGCGTTCACAGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGCTACT  >  NC_000913/1039881‑1040370                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     aTTCATCTGCGTTCACAGCTGGCGACCAAACGCGCGGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGGGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGt                                                                                                                                                                                                                                                 <  2:104557/251‑1 (MQ=255)              cacaGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTa                                                                                                                                                                                                                                     <  1:209773/250‑1 (MQ=255)                          cccaaagcgcTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTAGTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTACTTCCAATCCGTTAATGAAAATGGTGGCAGTGCTACCTGGTGCCTGGAAGAATAATTTTGTCGAGGCGGCCGGTTTGTGGAACTTCCCGCGGCTGGGGTTCTTCTGGCCATTGCTTAAGGTGATGGGGGTTTATTGTGGTTGCCCg                                                                                                                                                                                                                        >  2:220779/7‑251 (MQ=255)                                                 aTGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGcatc                                                                                                                                                                                                 >  1:114788/1‑251 (MQ=255)                                                          tttttGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTTTACTGCTCGCCCAGGGTGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGGTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCCTTGCTTACGGTGATGGCGGTTTATCGTGGTCGCCCGGGGTGGGGATTTTTGATGGCATCATTTATGcc                                                                                                                                                                                        >  2:128670/1‑250 (MQ=255)                                                               gCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTg                                                                                                                                                                                   <  2:114788/251‑1 (MQ=255)                                                                                     gTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCAt                                                                                                                                                             >  2:7125/1‑251 (MQ=255)                                                                                                                                                            ggCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACtt                                                                                      <  2:155995/251‑1 (MQ=255)                                                                                                                                                                     aCCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGc                                                                             <  2:6391/251‑1 (MQ=255)                                                                                                                                                                         ggTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGAc                                                                         <  1:128670/251‑1 (MQ=255)                                                                                                                                                                          atGCCTGGCTGACTACTTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCAGCTGCTGAGATTCTTCTGCACATTGCTTACGGTGATGGCGTTTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGCTGCACTTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCACGTTGTCATTCCGTCCAGCGTGAGTCCGATCTGCAGCCTGCCGTTGTGGGCAAGCACTTCCGGTCAGCCGACg                                                                        >  2:268779/2‑251 (MQ=255)                                                                                                                                                                            gCCTGGCTGAATACTTTTGTCGCGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCCGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCt                                                                      >  2:224689/1‑251 (MQ=255)                                                                                                                                                                                ggATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCTCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTTATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCGCGCTGTGCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCGCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAg                                                                   <  1:83295/250‑1 (MQ=255)                                                                                                                                                                                        aatTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTGTTGATGGCATCATTGATGCAATTTGGCGTGATGTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGTGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGt                                                           <  1:224689/250‑1 (MQ=255)                                                                                                                                                                                                 gAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGt                                                 <  1:7125/251‑1 (MQ=255)                                                                                                                                                                                                  aGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCAt                                                                >  2:147517/1‑235 (MQ=255)                                                                                                                                                                                                  aGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCAt                                                                <  1:147517/235‑1 (MQ=255)                                                                                                                                                                                                                                                aTTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGctact  >  1:91803/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     ATTCATCTGCGTTCACAGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGCTACT  >  NC_000913/1039881‑1040370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑