Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,546,601	C→T	I502I (ATC→ATT)	fimD →	type I fimbriae usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,546,601	0	C	T	100.0%	108.9 / NA	33	I502I (ATC→ATT)	fimD	type I fimbriae usher protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/15);  total (18/15)

GTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGGACGCTCAGCTATAGCCTGACGAAAA  >  NC_000913/4546372‑4546845                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     gTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTAtt                                                                                                                                                                                                                                 >  2:120982/1‑251 (MQ=255)      gggAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGGTGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAAGCACGAATATTCAGTTAGTGGGATACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGt                                                                                                                                                                                                                            >  1:191480/1‑251 (MQ=255)                       aCTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCg                                                                                                                                                                                                           <  1:59652/251‑1 (MQ=255)                                tctgtctgCGGATATGCCGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCCTGACGGACAATCGGTGCGTTTTCTCTATAGCAAATCGCTCAATGAATCAGGCACGAATATTCCGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGACGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTCTTCAGGTTAAGCCGAAATTCACCGACTAtgac                                                                                                                                                                                                   >  2:257692/1‑247 (MQ=255)                                       gtgGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCTAAATTCACCGACTATTACAACCt                                                                                                                                                                                              <  2:54623/248‑1 (MQ=255)                                             atGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGTTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATaa                                                                                                                                                                                     <  1:95998/251‑1 (MQ=255)                                                         aaTTCCACACTTCCCGCTGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGTGTTAACGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTAGTCAGGTTAAGGCGTTATTCACCGGCTATTAAAACCTCGCTtaaaaacacagaggga                                                                                                                                                                          >  2:194749/1‑236 (MQ=255)                                                             ccACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCATCGGATAGTGTAATTTCGCTGATACAATATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGTTTAAGCCGAAATCCACCGGCTATTACAACCTCGCTTATAACAAACGCGGGAAATTa                                                                                                                                                                     <  1:277482/251‑1 (MQ=255)                                                                     cccGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAc                                                                                                                                                             <  1:152319/251‑1 (MQ=255)                                                                      ccGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAcc                                                                                                                                                            >  1:252958/1‑251 (MQ=255)                                                                              aGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                                                                                                                                                                                       <  1:69285/216‑1 (MQ=255)                                                                              aGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                                                                                                                                                                                       >  2:69285/1‑216 (MQ=255)                                                                                                 cGGTGCGTTTTCTCTATAACAAATCGCTCAATGACTCAGGCACGAATATTCAGTTAGTGGGTTACCGATATTCGACCAGCGGATATTTTAATTTCGCTGATACAACCTACAGTCGAATGAACGGCTACAACATTGCAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACATCGCTTATAACAAACGCGGGTAATTACAACTCACCGTAACTCAGCAACTCGGGCGCACATc                                                                                                                                  >  2:93321/1‑250 (MQ=255)                                                                                                                                tGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACtt                                                                                                  >  1:233545/1‑251 (MQ=255)                                                                                                                                      aGGCACGAATAATCAGTTAGTGGGTTAACGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTAACTCAGCAACTCGGGCGAACATCAACACTGTATTTGAGTGGTAGCCATCAAACTAATTggg                                                                                            >  2:210423/1‑251 (MQ=255)                                                                                                                                            gAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAATTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACg                                                                                       >  1:64610/1‑250 (MQ=255)                                                                                                                                             aaTATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACg                                                                                       >  1:217538/1‑249 (MQ=255)                                                                                                                                                atTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATCTTTTAATTTCGCTGATACAACATACAGTCGAATGAATGTCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCGACTCGGGCGCACATCAACTCTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTa                                                                                   >  1:146862/1‑250 (MQ=255)                                                                                                                                                atTCAGGTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCCCCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTaa                                                                                  <  1:31278/251‑1 (MQ=255)                                                                                                                                                           gggTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGc                                                                       <  1:41365/251‑1 (MQ=255)                                                                                                                                                           gggTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGc                                                                       >  1:226615/1‑251 (MQ=255)                                                                                                                                                                   gTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTcc                                                                 >  1:127314/1‑249 (MQ=255)                                                                                                                                                                         cGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCAAGGCTgg                                                          >  2:169389/1‑250 (MQ=255)                                                                                                                                                                            ccAGCGGATATTTTAATTTCGCTGATACAACATACAGTCTAATGAATGGCTACAACATTGATACACATGTCTGAGTGATTCAGGTTAAGCCGAAATTCTCCGACTATTACTACCGCGCTTATAACAAACGCTGGAAATTACAACTCACCGTTACTCAGCAACTCGTGCGCTCAACAACACTGTATTTGAGTGGTTGCCATCAGACTGATTTGGGAACGATTAATGGCGAtttgtaatggcagtcttgagta                                                      <  2:272979/251‑22 (MQ=255)                                                                                                                                                                                        ttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAACTTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTc                                          >  1:38987/1‑251 (MQ=255)                                                                                                                                                                                                   gATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGGAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATc                                 <  1:106185/249‑1 (MQ=255)                                                                                                                                                                                                      acaacaTACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCaa                                                                                                                                                <  1:24920/135‑1 (MQ=255)                                                                                                                                                                                                      acaacaTACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCaa                                                                                                                                                >  2:24920/1‑135 (MQ=255)                                                                                                                                                                                                             aCAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCTCCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGGACGCt                      <  2:146372/250‑1 (MQ=255)                                                                                                                                                                                                              cAGTCGAATGAATGGCTACAACATTGAAACACAGGACGTAGTTATTCAGGTTAAGCCGAAAGTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGTCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGGACGCTCa                    <  1:194749/251‑1 (MQ=255)                                                                                                                                                                                                                        aatgGCTACAACATTGAAACACCGGACGGGGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCTCCGTTACTCAGCAACTCGGGCTCACATCAACACTGTATTTTAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGGACGCTCAGCTATAGCCt          <  1:214520/251‑1 (MQ=255)                                                                                                                                                                                                                          tggctccgacaTTGAAACACAGGACGGAGTTATTAAGGTTAAGCCGAAATTCACCGACTATTACAACCACGCTTATAACAAACGCGGGCAATCACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCAATCAAACTTATTGGGGAACGCGTAATGTCGATGAGCAATTCCAGTCTGGATTAAATACTGCGTTCGAAGATATCAACTGGACGCTCAGCTATAGCCTg         >  1:233505/9‑250 (MQ=255)                                                                                                                                                                                                                                caacaTTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGGACGCTCAGCTATAGCCTGACGaaaa  <  2:13596/251‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     GTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGGACGCTCAGCTATAGCCTGACGAAAA  >  NC_000913/4546372‑4546845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑