Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,532,409	T→G	intergenic (‑758/+28)	sgcX ← / ← yjhP	putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,532,409	0	T	G	100.0%	130.7 / NA	36	intergenic (‑758/+28)	sgcX/yjhP	putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/11);  total (25/11)

AAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTG  >  NC_000913/4532168‑4532619                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                   aaaCCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGccagccag                                                                                                                                                                                                            <  1:241221/250‑1 (MQ=255)             gCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAAc                                                                                                                                                                                               >  1:111622/1‑251 (MQ=255)                   tcCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTAAGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTACGCAAGCCTGACAGAAATGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCtgttg                                                                                                                                                                                         >  1:36689/1‑251 (MQ=255)                    cccAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGCAATACCGGGAACCtgttgt                                                                                                                                                                                        >  1:168689/1‑251 (MQ=255)                    cccAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCtgttg                                                                                                                                                                                         <  1:78752/250‑1 (MQ=255)                     ccAGGGATTGAGATTGCCGTTGATGTCGCTGCCTTACGCACAATATGGAGCAATGGCACTGGAAGCATCTGTGGCCGAAATCGGCATTCCGCACGCCTGACAGAACTGGTAATGTGGTGACATAGGATGCTCCGTATGGAGACATCTTGATTAATAAACATACTGAATATGTATTGGGCAGGGGATACATCAGATCAAAGAAAAGGAAAAGCATCGATAGTGTCAGCCAGAAATAACGGGAACCTgttgtt                                                                                                                                                                                       >  2:394/1‑251 (MQ=255)                      cAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTgttgtt                                                                                                                                                                                       >  2:205197/1‑250 (MQ=255)                       aGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGCGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTa                                                                                                                                                                                      >  2:199470/1‑250 (MQ=255)                               agaTTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGAt                                                                                                                                                                             >  2:225415/1‑251 (MQ=255)                                       gTTGCTGTCGCTGCCTTACGCTCAATATTGATCACTGGCACTGTGTGCATCTGCCGCCGACCTCGCCATTACGAAAGCCTGACAGAACTATACATGTTGTGCCATATGATGCTCCTAATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGAGCAGGTGCTACATCAGATCACAGCAAAGGTAAAGAATCGATATTGCCAGACAGAAATACCGGGAACCTGTTGTTACCGCGCGAGCAGTGCg                                                                                                                                                                      >  2:246948/1‑250 (MQ=255)                                               cGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGcc                                                                                                                                                              <  2:270276/250‑1 (MQ=255)                                                             caATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATTCCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTc                                                                                                                                               <  1:98668/251‑1 (MQ=255)                                                                tatTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCcg                                                                                                                                            >  1:178810/1‑251 (MQ=255)                                                                 atTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCcgc                                                                                                                                           >  1:120870/1‑251 (MQ=255)                                                                          tGGCACTGTGTGCGTCTGGCGCCGACATCGGCATTCCGCAAGCATGACAGAACTGTTCATGTTGTGACATCTGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAGTATGTATTGTGCAGGAGATACATCAGATCAAAGCAAAGGTAAATCCTCGATATTGCCAGCAAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGAGAACACGCCCCCGCCAAAGCATTCCCGCGCGTAggcc                                                                                                                                  >  2:180100/1‑249 (MQ=255)                                                                               cTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGACAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGAATTCCCGCGCGTAGGTCACGt                                                                                                                              >  1:69527/1‑250 (MQ=255)                                                                                  tgtgCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCg                                                                                                                          >  1:196050/1‑251 (MQ=255)                                                                                         cTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGgc                                                                                                                   >  2:42/1‑251 (MQ=255)                                                                                                 gACATCGGCCTTCCGCAAGCCTGACAGAACTGTTCATGTTGTTACATATGATGCTCCGTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTTAAGCCTCGATATTGCCAGCCAGAAAGACCGGGAACCTGTTGGTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGTTCACGTCGCGTTTCGTCGCGAGGtt                                                                                                           <  2:229853/251‑1 (MQ=255)                                                                                                         cATTCCGCAAGCCTGACAGAACTGTTCATGCAGTGCCATATGATGCCACTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCCGGTGATACATCAGATCAACGCAAAGGTAAAGCCTCGATATTGCCAGACAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCAAGCCAAAGCATTCCCGCGCGGCGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCgg                                                                                                   >  2:113758/1‑251 (MQ=255)                                                                                                          aTTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGACAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGc                                                                                                  >  1:110097/1‑251 (MQ=255)                                                                                                                cAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGAc                                                                                            <  1:202420/251‑1 (MQ=255)                                                                                                                    ccTGACAGAACTGTTCATGTTGTGACATATGACGCTCCTTAAGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGAAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCCATATTGACAGACAGACATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCAATCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTcg                                                                                        >  1:271858/1‑251 (MQ=255)                                                                                                                        aCAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCTATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTcggcgg                                                                                    <  1:14070/251‑1 (MQ=255)                                                                                                                               tGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAACCATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTc                                                                             >  2:244596/1‑251 (MQ=255)                                                                                                                                     tgttgtGACATCTGATGCTACTTATGGTGACATCTTGATTACTCAACCTCCTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGCTGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTc                                                                       >  2:36948/1‑251 (MQ=255)                                                                                                                                           gACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCTGCGGCGAAGTCATCGTCAGGAtt                                                                 <  2:60470/251‑1 (MQ=255)                                                                                                                                             cATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGGTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCg                                                               <  1:36948/251‑1 (MQ=255)                                                                                                                                                            aTGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCTGGAACCTGTTGTTACCGGGCGATGAGGTCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGGCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCAGCGTCAGGATTCGCTTCCAGTCAgcggc                                                <  2:89465/251‑1 (MQ=255)                                                                                                                                                                  aCATCTTGATTAATAAACATACTGAATATGTATTGTGCCGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGCAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATgg                                          >  1:88456/1‑251 (MQ=255)                                                                                                                                                                        tGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCGCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGTTCACGTAGCGTTGCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCAGCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAg                                      <  2:177394/249‑1 (MQ=255)                                                                                                                                                                          aTTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCAt                                  >  2:3568/1‑251 (MQ=255)                                                                                                                                                                                      actgactctatATTGTGCAGGTGATACATCAGATCAACGCACAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGAGCGAACACGCCCCAGCCAAAGCATTCCAGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCCTGGTCAGCCATTTTGCGGCTTc                       >  2:112332/11‑250 (MQ=255)                                                                                                                                                                                               gTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTcc             >  1:165301/1‑251 (MQ=255)                                                                                                                                                                                                   tgtgCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGc         >  1:81497/1‑251 (MQ=255)                                                                                                                                                                                                          gTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTg  >  1:119659/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                   AAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTG  >  NC_000913/4532168‑4532619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑