Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	42,108	G→A	intergenic (‑177/‑295)	caiT ← / → fixA	L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	42,108	0	G	A	100.0%	64.0 / NA	21	intergenic (‑177/‑295)	caiT/fixA	L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/13);  total (8/13)

ATGCCGACGATTATTAACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAGGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTTATTTCAAAATTAAAGGG  >  NC_000913/41861‑42338                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       aTGCCGACGATTATTAACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGc                                                                                                                                                                                                                                     <  1:6550/251‑1 (MQ=255)      gactattattAACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAAGTATTATTAGCCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTTATTGGTAGCACATTTTTGTTTCGGGTGAATAGAAGGCGtttt                                                                                                                                                                                                                                <  1:128943/247‑1 (MQ=255)                                           aTTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGc                                                                                                                                                                                             <  1:203402/249‑1 (MQ=255)                                             tCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAATACGAATATTTATTTTGCGTTCCCTTATGTTTTATTTTTGTTAACATTTCATATAATTAGTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGTTGAATAGAAGGCGTTTTTTCTTTAATTTTGTTTAATAATCAGTTTTTTATGctct                                                                                                                                                                                          <  1:144813/250‑1 (MQ=255)                                                    tttCTCTTTTCATTCTTCATGCGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTtgtg                                                                                                                                                                                    >  1:261906/1‑249 (MQ=255)                                                          ttttCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTaaaa                                                                                                                                                                             >  1:97857/1‑250 (MQ=255)                                                                    tcATGAGTTAATTCCACTGTGAAAACGTATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTGTTGTTTCGGGTGAATAGAAGGCGTTTTTTCTTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATc                                                                                                                                                                    <  1:236176/249‑1 (MQ=255)                                                                          gTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGTCGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTTTGATTGGTATCTCATTTGTGTTGCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTc                                                                                                                                                            <  1:51721/251‑1 (MQ=255)                                                                                   aCTGTGAAAACGAATATGTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACACGTTAATGACTAACTAATAATGGGTATTCAATAAGCTGTCTTCTGTGATTGGTATCACATTATTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTAACTTTCAATATTGGt                                                                                                                                                   >  2:237116/1‑251 (MQ=255)                                                                                    cTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTg                                                                                                                                                  >  2:148785/1‑251 (MQ=255)                                                                                    cTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTg                                                                                                                                                  >  1:90258/1‑251 (MQ=255)                                                                                      gtgAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGAt                                                                                                                                                >  2:225685/1‑251 (MQ=255)                                                                                        gAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATATTGATTAATAATCAGTTTGTTATGCGCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATcc                                                                                                                                              <  2:64102/251‑1 (MQ=255)                                                                                                                          ttagttttGTTAACATTTAATATAATTATTAGTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCGGTATTCTGTGATTGTAATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGAGTAATAATCAGGTTGTTATGCTCTGTTGAGAGTAAAAAATAACATCTGGCGTTCAATATTGGTTATCCATAAATCAATATTGaaaaatagtttgtgctacg                                                                                                             <  2:193440/246‑16 (MQ=255)                                                                                                                                                        attaACCTCGTGGACGCGTTAATGGCTAACTACCAATGGGCATCCAAAAAGCCGTATTCAGTGATTGGAATAACATTTTTGTTTCGGGTGACAAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGATCTGTTGTGAGTAAAAAATAACATCTGCCTTTATATATTGGTGATCCATAACACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTacc                                                                              >  1:14718/1‑249 (MQ=255)                                                                                                                                                                       gcgTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCGTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTgg                                                               <  1:244134/251‑1 (MQ=255)                                                                                                                                                                                                             gTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCTTGTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTTGCTTTCAATATTGTTGATCCATAAAACAATATTGAAAATTTCTTTTTTCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTGATCTGCGTGTGACATTTTCAATATTGGTGAt                            <  1:208852/248‑1 (MQ=255)                                                                                                                                                                                                              tATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTaa                         >  1:198188/1‑250 (MQ=255)                                                                                                                                                                                                                             aTCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTTATTTCaaaa          <  1:225685/250‑1 (MQ=255)                                                                                                                                                                                                                             aTCACATTGTTGTTTCGGGTGAATAGAAGGCGTTTTTTCTTTAATTTTGATTAATAATCATTTTGTGATGCGCTGTTGTTAGTAAAAAATTACATCTGCCTTTCAATATTGGTTATCCATAAAACAATATTGCATATTTCTTTTTGCTTCGCCGTGTTTTCAATATTGGTGAGGATCTGAACAATATTGAAATTTGGAATTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTAATTTCaaaa          <  2:268309/250‑1 (MQ=255)                                                                                                                                                                                                                                     tttGTTTCGGGTGAATAGAAGGTGTTTTTTCGTTAATTTTGATTAAGAATCAGGTTGGTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTCTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTTATTTCAAAATTAAAggg  <  1:42395/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       ATGCCGACGATTATTAACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAGGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTTATTTCAAAATTAAAGGG  >  NC_000913/41861‑42338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑