Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,737,689	IS150 (+) +3 bp	intergenic (+43/‑54)	pheL → / → pheA	phe operon leader peptide/fused chorismate mutase/prephenate dehydratase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2737689 =	0 (0.000)	14 (0.530)	14/488	0.7	100%	intergenic (+43/‑56)	pheL/pheA	phe operon leader peptide/fused chorismate mutase/prephenate dehydratase
	?	NC_000913	= 3722075	NA (NA)					noncoding (1443/1443 nt)	IS150	repeat region
*	?	NC_000913	= 2737691	0 (0.000)	23 (0.870)	23/486	0.3	100%	intergenic (+45/‑54)	pheL/pheA	phe operon leader peptide/fused chorismate mutase/prephenate dehydratase
	?	NC_000913	3720633 =	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

CGTAATTAATCTTTCCAGCAAATCGCGTTCACGATCAATATCACGTACCGGGCGATGCGAGAGCAGTTTGGCTTTTCCCACCTCGACGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2737927‑2737689 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATATTACTGAACTCATCAAGATAAAAACACTCCGACTTTAAGGTTCCAAAGAAACACTCCACCACAGCATTATCCAGACAATTGCCTTTTCT  <  NC_000913/3722075‑3721834                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   CGTAATTAATCTTTCCAGCAAATCGCGTTCACGATCAATATCACGTACCGGGCGATGCGAGAGCAGTTTGGCTTTTCCCACCTCGACGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCC                                                                                                                                                                                                                                        <  2:172185/251‑1    AATTAATCTTGCCAGCAAATCGCGTTCACGATCAATATCACGTACCGGGCGATGCGAGAGCAGTTTGGCTTTTCCCACCTCGACGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCTTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAA                                                                                                                                                                                                                                      <  2:58251/250‑1                     AATCGCGTTCACGATCAATATCACGTACCGGGCGATGCGAGAGCAGTTTGGCTTTTCCCACCTCGACGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAAC                                                                                                                                                                                                                    <  2:191813/251‑1                                          CACGTACCGGGCGATGCGAGAGCAGTTTGGCTTTTCCCACCTCGACGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATT                                                                                                                                                                                               >  1:113187/1‑251                                               ACCGGGCGATGCGAGAGCAGTTTGGCTTTTCCCACCTCGACGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAA‑GTTGGACAGTTA                                                                                                                                                                                                                       >  1:154571/1‑221                                               ACCGGGCGATGCGAGAGCAGTTTGGCTTTTCCCACCTCGACGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAA‑GTTGGACAGTTA                                                                                                                                                                                                                       <  2:154571/221‑1                                                    GCGATGCGAGAGCAGTTTGGCTTTTCCCACCTCGACGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCA                                                                                                                                                                                     <  1:29154/251‑1                                                        TGCGAGAGCAGTTTGGCTTTTCCCACCTCGACGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTG                                                                                                                                                                                 >  2:242469/1‑251                                                                                                                                             TGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGCTGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTAT                                                                                            >  2:13438/1‑251                                                                                                                                                      CTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATAGATGCCGTAACAGCATCCTTCAGTGCGCTTATAGTACTG                                                                                     <  2:113187/249‑1                                                                                                                                                                                      ATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATATTACTGAACTCATCAAGATAAAAACACTCCGACTTTAAGG                                                   <  2:53931/251‑1                                                                                                                                                                                                       TTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAACCACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATATTACTGAACTCATCAAGATAAAAAAACTCCGACTTTAAGGTTCCAAAGAAACACTCC                                  >  1:202836/1‑251                                                                                                                                                                                                                 AAAAGGCCCCCCGATTTGGGAGGCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATATTACTGAACTCATCAAGATAAAAACACTCCGACTTTAAGGTTCCAAAGAAACACTCCACCACAGCAT                        >  1:262212/1‑251                                                                                                                                                                                                                                       GCCTTATTGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATATTACTGAACTCATCAAGATAAAAACACTCCGACTTTAAGGTTCCAAAGAAACACTCCACCACAGCATTATCCAGACAATTGCCTTTTCT  <  2:54839/251‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   CGTAATTAATCTTTCCAGCAAATCGCGTTCACGATCAATATCACGTACCGGGCGATGCGAGAGCAGTTTGGCTTTTCCCACCTCGACGGCCAGTTCGCGCCGTTCTGCCAGTAACGCTAATAATTTTTCATCCAGCGCGCTGATTTTCTCTCGCAGCGCCAGTAACGGGTTTTCCGATGTCATAGTGTTGCCTTTTTGTTATCAATAAAAAAGGCCCCCCGATTTGGGAGGCCTTATTGt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2737927‑2737689 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCGCTTATATTACTGAACTCATCAAGATAAAAACACTCCGACTTTAAGGTTCCAAAGAAACACTCCACCACAGCATTATCCAGACAATTGCCTTTTCT  <  NC_000913/3722075‑3721834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.