Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	399,639	+A	coding (47/213 nt)	yaiZ →	DUF2754 domain‑containing protein YaiZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	399,639	1	.	A	100.0%	25.1 / NA	9	F16Y (TTC→TAC)	yaiZ	DUF2754 domain‑containing protein YaiZ
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (4/5);  total (4/5)

CATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTT‑CGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGA‑ATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGAC  >  NC_000913/399403‑399866                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                     cATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTACGCCATTGGCCt                                                                                                                                                                                                                          <  1:222138/250‑1 (MQ=255)                                                   ccctaaGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAgg                                                                                                                                                                       <  1:75971/247‑1 (MQ=255)                                                                     gTTGTGAGTCACAAAATGGCAACCTTAAGTTAACTTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGt                                                                                                                                                     >  1:12043/1‑251 (MQ=255)                                                                                   aaTGGCAACCTTCAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGCTTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACgg                                                                                                                                       >  2:27940/1‑250 (MQ=255)                                                                                       gCAACCTTAAGTTAAATTTAGTAACAATTTCGAAAATTGTGTTTTCACCTCAGACGGTTGTTGCAGAATATGCAAGGATGTGGTTTTTCGTTATCGGAGCTTCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGTCTGTTCTATGCGTTACTCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTTCTGTGATGTGAAGCTAAAGGTTGTCAGACCTATGCCGTCGGTCTGGTGACGTGGGt                                                                                                                                   <  1:265157/251‑1 (MQ=255)                                                                                         aaCCTTAAGTTAAATTTATTAACAATTACGATAATGTTGTATACACCACAGACGGTTGTTGCAAAATATGCACGGCTGTTGTTTTTCGTTACCGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTACGCCATTGCCATTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAATGTTTGCAGACCTATGCCTTCGGGCTGGATACGTGGGTgg                                                                                                                                 >  2:168992/1‑250 (MQ=255)                                                                                                                                                                                                                   ccgccgTGCCTGGCACTACTATGCGTTACGCCATTGGCCTTCTATTCATTCTTAATGGCGTGGTGGGGTTACTTGGATTTGAAGCAAAAAGTTGGCAGACCTATGCCGTCGGTCTGGTGCCGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGCTTATTCGTTGCCCCGCTGAAGGAACTGGAAACGCCCCAAACGACAT‑TTATCGGCCCTTCAAATATTACCTTTTGGTTTTTCATGAGTTATcagccccg      >  2:209525/1‑244 (MQ=255)                                                                                                                                                                                                                     gccgTGACTGTCACTACTATGCGTTACGCCATTGTCCTTATATTCATTCTTAATGGCGTGTGGGGGTTACTGGTATTTGAAGCAAAAGGTTGTGAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGTTTTGATTATTCGTCGTCGCTATGAAGAATCTGATAACGCCCAATAAGA‑ATATTATCGGTCGTTTAAATATTGCATTGTGTGTTTCAATCCTTTATCCTCAGCGTg    <  2:263271/251‑1 (MQ=255)                                                                                                                                                                                                                       cgTGACTGGCACTACTATGCGTTACGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAATGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTAGTCGTCTTCGCGATGAAGAAACTGAAAACGCCCAATAAGA‑ATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGAc  <  1:119369/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                     CATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTT‑CGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGA‑ATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGAC  >  NC_000913/399403‑399866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑