Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,282,332	(A)5→6	coding (744/1293 nt)	yjcF ←	pentapeptide repeat‑containing protein YjcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,282,332	1	.	A	88.2%	50.6 / 1.7	17	coding (744/1293 nt)	yjcF	pentapeptide repeat‑containing protein YjcF
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base A (5/10);  total (6/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

ATAACCATTTCGAGGAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAA‑AA‑TTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAAT  >  NC_000913/4282108‑4282573                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  ataACCATTTCGAGGAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAA‑AAATTTGAATAATCAAGGAAGGTAttt                                                                                                                                                                                                                           <  1:90304/250‑1 (MQ=255)            gAGGAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAA‑AAAGTTGAATAATCAAGGAAGGTAGTTTTAATATTGGct                                                                                                                                                                                                               <  1:39201/251‑1 (MQ=255)                                                 cTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAA‑AAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCAt                                                                                                                                                                            >  2:59222/1‑249 (MQ=255)                                                   aTCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGATAAGCTAAGTTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAA‑AAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGc                                                                                                                                                                         <  1:262344/250‑1 (MQ=255)                                                                                    aTTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAA‑AA‑TTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTATAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTa                                                                                                                                      <  1:138854/251‑1 (MQ=255)                                                                                       cAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAA‑AAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATc                                                                                                                                    <  1:141757/251‑1 (MQ=255)                                                                                                acGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAA‑AAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGaaa                                                                                                                           <  2:113619/251‑1 (MQ=255)                                                                                                       aGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAA‑AAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGt                                                                                                                    >  2:183214/1‑251 (MQ=255)                                                                                                           cAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAA‑AAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCAt                                                                                                                 <  1:4945/250‑1 (MQ=255)                                                                                                                  ttCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAA‑AAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAAt                                                                                                          >  1:214406/1‑250 (MQ=255)                                                                                                                                            agaAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAA‑AAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCAt                                                                               >  2:114515/1‑251 (MQ=255)                                                                                                                                                                          tatatGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCAATATAAA‑AAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACat                                                 >  2:167058/1‑251 (MQ=255)                                                                                                                                                                                     aTTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAA‑AAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTcc                                       <  1:254596/250‑1 (MQ=255)                                                                                                                                                                                                   aTTTACCTCAGCCATGTATGCCATATAAA‑AAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAAGAGCGttt                        <  1:61113/251‑1 (MQ=255)                                                                                                                                                                                                               cATGTATGCCATATAAA‑AAATTTGAATAATCAAGGAAGGTATTTTTAATAGTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCTGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCaa            <  1:264506/251‑1 (MQ=255)                                                                                                                                                                                                                    aTGCCATATAAA‑AAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGc       <  1:134996/251‑1 (MQ=255)                                                                                                                                                                                                                         atatAAACAA‑TTTGAATAATCAAGGAAGGTATTTTTAATAATGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAAt  >  1:204344/1‑251 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  ATAACCATTTCGAGGAACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAA‑AA‑TTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAAT  >  NC_000913/4282108‑4282573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑