Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,336,4110GA97.7% 142.6 / ‑2.8 43coding (84/1260 nt)murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (18/24);  minor base . (1/0);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01
*NC_0009133,336,4120AT93.0% 131.0 / ‑2.5 43I28N (ATC→AAC) murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base T (17/23);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTT  >  NC_000913/3336175‑3336626
                                                                                                                                                                                                                                            ||                                                                                                                                                                                                                       
cccTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGagcagc                                                                                                                                                                                                           <  1:329430/251‑1 (MQ=255)
 ccTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCAGTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGGATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAgcagca                                                                                                                                                                                                          <  1:333885/251‑1 (MQ=255)
      ccAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCAttttt                                                                                                                                                                                                     >  1:98248/1‑251 (MQ=255)
       cAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGCGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCAttttt                                                                                                                                                                                                     >  1:110152/1‑250 (MQ=255)
               gcTACCAGCTGCCCCGTCGCCCAGATAGAAGCACGCATGGTTTTAACCAGAGCGTAAGTTGCGCTGAATACATTATCGTCTCGGTCAGCAATATGCACAGAACCCTTACGTACTACTTTCGCACCCAGCTGTCTGAGCAGCTTCATTGATGTATCGACGTCTTTCAGTATCGGGAAGTTCGGGGTCTCTTCCGGTTCGTCCGCTGGTAGTGCTGCAAAAAGATTT‑GGCATAGCTGCATTTTGAGCGCCGGa                                                                                                                                                                                            <  2:274523/251‑1 (MQ=255)
               gcTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGGGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCgg                                                                                                                                                                                             >  1:164013/1‑250 (MQ=255)
                cTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGa                                                                                                                                                                                            >  1:277996/1‑250 (MQ=255)
                         gCCCCAGCGCCCAGATAGAAGCACGCATTGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCCCCCAGCTGGCTTATCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACt                                                                                                                                                                                  <  1:110364/251‑1 (MQ=255)
                          ccccAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACtt                                                                                                                                                                                 >  1:251340/1‑251 (MQ=255)
                            ccAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCAGCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTTCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCg                                                                                                                                                                               <  2:132015/251‑1 (MQ=255)
                                         aGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCTCCCAGCTGGCTTAGCGTCTTCATTTAGGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTGCCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCATGGCTGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCg                                                                                                                                                                  <  1:396595/251‑1 (MQ=255)
                                          taaGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATCCATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCCCCCAGCGGGCTGAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTGGGGGACGGTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGagc                                                                                                                                                                                                              <  2:47669/205‑1 (MQ=255)
                                          gAAGCACGCATGGTTTTAACCAGATCGTAAGGAGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACACAACCATTACGTTCTACTTTCGCACCAAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGagc                                                                                                                                                                                                              >  1:47669/1‑206 (MQ=255)
                                                   aTGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTg                                                                                                                                                        <  1:117703/251‑1 (MQ=255)
                                                     ggTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTCCGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGa                                                                                                                                                       >  1:362574/1‑250 (MQ=255)
                                                     ggTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGACGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCGGCTGGCTTATCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGAGCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGaa                                                                                                                                                      <  2:169428/251‑1 (MQ=255)
                                                        tttAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCCGTTTCGGGACGTTCTGGATCTCTACCGGTTATTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAacac                                                                                                                                                   >  2:432748/1‑251 (MQ=255)
                                                                gATCGTAAGGGGCGCAGAATACATTAACGACGCGGGCATCAATATGCACAGAACCCTTACGTTCTACTTTCGCCCCCAGATGGCATAGAAGCTTCATTGATGTATCGCCGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGATCATCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCATCGCATCGTTTTTAGCGCCGGACATTGGGACTTCGCCCTGCAGCTTCGTTGGCCCCTGAACACgcattttc                                                                                                                                           >  2:367011/1‑244 (MQ=255)
                                                                         gTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCAttt                                                                                                                                    >  1:387301/1‑249 (MQ=255)
                                                                         gTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCAGTTTTAGCGCCGTAAATTGTGACTTCGCCCGGGAGCGTCGTTGGCCCCTTAACACGAAATTTATCCATTTAg                                                                                                                                  <  2:185959/251‑1 (MQ=255)
                                                                           gcgcAGAATACATTAACGGCGCGGGCATCAATATGCACAGAACCATTACGTTCTACGTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGtt                                                                                                                                <  1:125367/251‑1 (MQ=255)
                                                                            cgcAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGttt                                                                                                                               >  1:308010/1‑251 (MQ=255)
                                                                            cgcAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGttt                                                                                                                               <  2:277996/251‑1 (MQ=255)
                                                                             gcAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTg                                                                                                                              <  2:308010/251‑1 (MQ=255)
                                                                             gcAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTg                                                                                                                              >  1:209212/1‑251 (MQ=255)
                                                                              cAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAACGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGt                                                                                                                             >  1:436132/1‑251 (MQ=255)
                                                                                       ttAACGTCGCGGGCATCAATGTGCACAGCACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGCCGTTCTGGCTCTCTACCGGTTATTCCGCCAGTAGTGCGGCACAAAG‑ATACGGCCGAGACGCATTTTTAGCGCCGGTAATTGAGACTTAGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGtt                                                                                                                     >  2:156805/1‑250 (MQ=255)
                                                                                          aCGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGTAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACa                                                                                                                 <  1:282570/251‑1 (MQ=255)
                                                                                           cGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGAAGCTTCATTGATGTATCGACGTCTTTCAGTTTAGGGACGTTATGGAGCTATACCGGTTCTTCCGCCAGTAGTGCGGAAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAAATGTGACTTCGCCCTGGAGATTCGTTGGCCCCTGAAAACGAACTTTATCCATTTAGTTTGTTCTCAGTTAACa                                                                                                                 >  1:222726/1‑250 (MQ=255)
                                                                                                 gggCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCATCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCata                                                                                                          <  1:352019/251‑1 (MQ=255)
                                                                                                  ggCACCAATATGCACTTACCCAATACGTGCTACTTTCGCACCCAGATGGCGTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGGCGTGCTGGATCTCTACCGGTTCGTCCGCCAGTAGCGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCTGCGGAAATTGTGAGTTCTTCCTGTAGCTTCGTTGTCCACTGAACACGAAGTTGATCCATGTAGTTTGTTCTCAGTTAACATTTCatat                                                                                                         <  2:49629/251‑1 (MQ=255)
                                                                                                                     ccATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGcc                                                                                      >  1:221603/1‑251 (MQ=255)
                                                                                                                                 aCTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCGGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGt                                                                                                                      <  1:403531/207‑1 (MQ=255)
                                                                                                                                 aCTTTCGCACACAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGt                                                                                                                      >  2:403531/1‑207 (MQ=255)
                                                                                                                                   tttCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGc                                                                        >  1:376665/1‑251 (MQ=255)
                                                                                                                                                      agcagcTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATcgcg                                                     <  1:141076/251‑1 (MQ=255)
                                                                                                                                                        cagcTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTAACGGTTCTTCCGCCAGTAGTGCGGCAAAAAGAATA‑GGCAGAGCAGCATTTTTAGCGCCGGAAGTTGTGGCTTCGACCTGGAGCGTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCAGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATcgcgcg                                                   >  1:1736/1‑251 (MQ=255)
                                                                                                                                                                     gTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCGACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAg                                       <  2:251340/250‑1 (MQ=255)
                                                                                                                                                                             gTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACg                              <  1:386573/251‑1 (MQ=255)
                                                                                                                                                                                                    ggATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTTCCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATg       <  1:422238/251‑1 (MQ=255)
                                                                                                                                                                                                          ctACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGc      <  1:371377/246‑1 (MQ=255)
                                                                                                                                                                                                          ctACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGc      <  1:121714/246‑1 (MQ=255)
                                                                                                                                                                                                            tccGGTTCTTCCGCCCGTAGTGCGGCAAAAAGAATA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGtt  <  2:98248/247‑1 (MQ=255)
                                                                                                                                                                                                                                            ||                                                                                                                                                                                                                       
CCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATA‑GGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTT  >  NC_000913/3336175‑3336626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑