Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,111,7270CT97.3% 119.7 / ‑3.6 38P289M (CCG→TTG) opgHosmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (23/13);  total (23/15)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01
*NC_0009131,111,7280CT97.4% 131.1 / NA 38P289M (CCG→TTG) opgHosmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (23/14);  minor base G (0/1);  total (23/15)
Fisher's exact test for biased strand distribution p-value = 3.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGA  >  NC_000913/1111504‑1111964
                                                                                                                                                                                                                               ||                                                                                                                                                                                                                                             
gggCTTCCTGAAACTGCTTATTGGTCGCGATACATAAAGTATATCTGCGTCACCAGTTGGCGATGAACCATTAAACACGGAGAATCCCACGGCGTTGATCATGCCTATCTGTAACGCAGAAGTGAACCGTGTTATTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGCCAGTTATAACTTGGATATCTGCG‑TCGCAGAGCaaaac                                                                                                                                                                                                                     >  2:413906/1‑249 (MQ=255)
           aaCTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCtggatgg                                                                                                                                                                                                           >  2:308309/1‑249 (MQ=255)
                    ttGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTAAATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCt                                                                                                                                                                                                >  1:171820/1‑251 (MQ=255)
                                aaTACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGCGCTTATCGCTGAAGTCGGTggg                                                                                                                                                                                    >  2:14413/1‑250 (MQ=255)
                                    cAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGa                                                                                                                                                                                  >  1:1278/1‑249 (MQ=255)
                                     aGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGACTGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGATTATCGCTGAAGTCGGTGGCGAAgg                                                                                                                                                                               >  2:111066/1‑251 (MQ=255)
                                      gTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACAGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAggg                                                                                                                                                                              >  1:236882/1‑250 (MQ=255)
                                                  cAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGACGTCGGTGGCGAAGGTCAGATTTTCTAt                                                                                                                                                                  >  2:68233/1‑251 (MQ=255)
                                                  cAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTAt                                                                                                                                                                  <  1:352880/251‑1 (MQ=255)
                                                                gAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTcg                                                                                                                                                    >  1:347821/1‑251 (MQ=255)
                                                                  aCCATTAAACCAGGGGCATCGCACGGCGTTGACCATGCCTATCTGTAACGAAGACGTGCACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGTGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGCAGGTCAGATTTTCTATCGCCGCCGCCGTcgcc                                                                                                                                                  >  2:96851/1‑251 (MQ=255)
                                                                        aaaCCCGGGGCATCGCCCGGGGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAGTGCCACCGGGAATGCCAAGCACTTTGATGTCTACATTCTTAGTGACAATTATAACTTGGATATC‑GCGGCCGCAGAGCAAATAGCCCGGATGGAGCTGATCGCTGAAGACCGTGGCGTAGGTCAGATTGTCTAGCGCCGCCGCGGGCTCCGCGTg                                                                                                                                             <  2:88519/250‑1 (MQ=255)
                                                                                 gCATCGCACGGAGTTGATCATGCCTATCTGTAACGAAGACGTGAAACGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGATATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGAGAAGGTCCGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTaaa                                                                                                                                    >  2:246848/1‑250 (MQ=255)
                                                                                          ggCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCg                                                                                                                                        <  2:67998/237‑1 (MQ=255)
                                                                                          ggCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGGGAAGCg                                                                                                                                        >  1:67998/1‑237 (MQ=255)
                                                                                          ggCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCAACCGGGCATGCCAAACACGTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCCGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGACGGACAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGGAAAAGCGGTAat                                                                                                                           >  2:145625/1‑250 (MQ=255)
                                                                                               tGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGa                                                                                                                      <  1:105920/250‑1 (MQ=255)
                                                                                                  gcATGCCTATCTGTAACGAAGACGCGACCCGTGTTTTTGCTGGCCGGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACGCTTTGATGTCTACATTCTTAGTGACAGGTATAACTTGGATATCTGCG‑TCGCAGAGCAAACAGCCTGGATGGAGCTTATCGCTGAAGTCGATGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCTCGTGAAGAGTCAAAGCGGGAAAACCGATGAc                                                                                                                  >  2:364693/2‑251 (MQ=255)
                                                                                                   cATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGAc                                                                                                                  <  1:111066/250‑1 (MQ=255)
                                                                                                       ccTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTg                                                                                                             >  2:312346/1‑251 (MQ=255)
                                                                                                         tATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGCTGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGcc                                                                                                           >  1:17302/1‑251 (MQ=255)
                                                                                                          aTCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGcc                                                                                                           <  1:91703/250‑1 (MQ=255)
                                                                                                          aTCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGc                                                                                                            <  1:401262/249‑1 (MQ=255)
                                                                                                                             aCCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCTACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTAc                                                                                       >  1:400219/1‑251 (MQ=255)
                                                                                                                                          tGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACAtggtgg                                                                          >  1:122806/1‑251 (MQ=255)
                                                                                                                                           ggCCTGCGGGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATAGCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATggtggt                                                                         <  1:266782/251‑1 (MQ=255)
                                                                                                                                                    gCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGc                                                                >  1:89329/1‑251 (MQ=255)
                                                                                                                                                           gggAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCg                                                         >  1:347198/1‑251 (MQ=255)
                                                                                                                                                                        aGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCT‑ATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGa                                           <  1:79858/251‑1 (MQ=255)
                                                                                                                                                                            aCCGGGAATGCCATCCACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTAGGTAATGACCGGTGCttg                                        >  2:138508/1‑251 (MQ=255)
                                                                                                                                                                            aCCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGAttg                                        <  1:161827/251‑1 (MQ=255)
                                                                                                                                                                                     gCCAAACACTGTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATAGCGAGGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGGGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCg                                <  2:434798/250‑1 (MQ=255)
                                                                                                                                                                                               ttGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTgc                       <  1:12049/249‑1 (MQ=255)
                                                                                                                                                                                                        aCATTCTTAGTGACAGGTATAACGTGGAGATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTGCTGCCGTCGCGGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATgg              <  2:122806/249‑1 (MQ=255)
                                                                                                                                                                                                               tAGTGACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCa        <  1:308309/248‑1 (MQ=255)
                                                                                                                                                                                                                aGTGACAGTTATAACTGTGAGATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCTCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAAccc    <  1:127455/251‑1 (MQ=255)
                                                                                                                                                                                                                 gTGACAGTTATAACTTGCATATCTGCG‑TCGCCGAGCAAAAAGCCTGGATGCAGCTTATCGCTGCAGTCGGTGGCGAAGGTCAGCTTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGAATTCTGCCGTCCCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGCGATTGTTTGTGCGGGCTGGTGCGCATGACGGAAGCCaaaacg   >  2:396516/1‑247 (MQ=255)
                                                                                                                                                                                                                   gACAGTTATAACTTGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGa  >  1:316415/1‑250 (MQ=255)
                                                                                                                                                                                                                               ||                                                                                                                                                                                                                                             
GGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCG‑TCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGA  >  NC_000913/1111504‑1111964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑