Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,084,903	T→G	N307T (AAC→ACC)	fdoG ←	formate dehydrogenase O subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,084,903	0	T	G	100.0%	251.9 / NA	70	N307T (AAC→ACC)	fdoG	formate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/39);  total (31/39)

GGAACGCGTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACG  >  NC_000913/4084661‑4085148                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      ggAACGCGTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAg                                                                                                                                                                                                                                               <  1:433192/251‑1 (MQ=255)      gcgTCTTTTGGCGTACCACAGATGTTTTCACCCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGTGGTGGTATAGGCTTc                                                                                                                                                                                                                                          <  2:182789/251‑1 (MQ=255)        gTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCgg                                                                                                                                                                                                                                        >  1:314972/1‑251 (MQ=255)            tttGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGgtgt                                                                                                                                                                                                                                     <  1:33881/250‑1 (MQ=255)            tttGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGGATAGGCTTCGgtgt                                                                                                                                                                                                                                     >  1:251555/1‑250 (MQ=255)               ggCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTAtt                                                                                                                                                                                                                                  <  1:239103/250‑1 (MQ=255)               ggCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTc                                                                                                                                                                                                                                 <  1:201643/251‑1 (MQ=255)               ggCGTACAACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATAGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGATTCGGTGTATTc                                                                                                                                                                                                                                 >  1:312059/1‑251 (MQ=255)                      cacaGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTg                                                                                                                                                                                                                          <  1:381073/251‑1 (MQ=255)                          gATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCTTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGGATTCGCGGTTGAAtt                                                                                                                                                                                                                      <  1:146242/251‑1 (MQ=255)                               tttCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTc                                                                                                                                                                                                                  <  1:207420/250‑1 (MQ=255)                                ttCAACCACATCTGGCGTGTTGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTGTTTCCGCGTCGTAGCCGGTTAACAGGCCATCTTCAAAGCCTTAATCCTCACGCACTATCAGGCGGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCa                                                                                                                                                                                                                 <  1:221931/250‑1 (MQ=255)                                  cAACCACATCTGGCGTGTAGCGGGAAACTTGCTGTTTCAGCAAGTGCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTg                                                                                                                                                                                                              <  2:186157/251‑1 (MQ=255)                                        caTCTGGCGTGTAGCGGGAAACGTGCTTTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTcagc                                                                                                                                                                                                        <  1:328538/251‑1 (MQ=255)                                        caTCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTcagc                                                                                                                                                                                                        <  1:253386/251‑1 (MQ=255)                                             ggCGTGTGGCGGGAAACGTGCTGTTTCGGCAAGGTCCACACGCAGCGCGGATGTTGCAGCGTGTTTTCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTTCTTTCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTGCAAAGCCGTAATCCTCCCGCTCGATCATGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGt                                                                                                                                                                                                    <  2:312059/250‑1 (MQ=255)                                             ggCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACCCGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTa                                                                                                                                                                                                   <  1:357298/251‑1 (MQ=255)                                             ggCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGGTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTACGGTGTATTCGCGGTTGAATTTTTCATTGGTcagcag                                                                                                                                                                                                      <  1:89842/248‑1 (MQ=255)                                                    aGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAAt                                                                                                                                                                                            <  2:304597/251‑1 (MQ=255)                                                      cGGGAAACGTGCTGTTTCAGCAAGATCCACACGCAGCGCGGATGTTGCCGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCAAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCCCGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCTTTGTTCAGCAGGGACAGCAATAc                                                                                                                                                                                          >  2:256418/1‑251 (MQ=255)                                                          aaaCGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCt                                                                                                                                                                                      >  1:357472/1‑251 (MQ=255)                                                             cGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGGGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGAc                                                                                                                                                                                   >  2:248866/1‑251 (MQ=255)                                                               tGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGATGCAGCGTGGTATCGCGTTTGACGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACCGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATCGGCTTCGGTGTATTCGCGGCTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGAc                                                                                                                                                                                   >  1:211623/1‑249 (MQ=255)                                                                 cTGTTTAAGCAAGTTCCACACGCAGCGCGGACGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAAGAGGATTTATAGTACTTGCGTTTTTCCGAGTCGTAGCAGGTGAACTGGCCATCTTCAAAGCCGAACTCCTCACGCACGATCAGGCTGGCGGTGGTAAAGGCTTCGGGGTATTCGCAGTTGAATTTTTCCTTGTTCAGTAGGTACAGCAATACCCCTGTCAgg                                                                                                                                                                                >  1:186514/1‑250 (MQ=255)                                                                      tCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGGGGTATAGGCTTCGGTGTATTCGCGGTTGACTTTTTCATTGTTACGCAGGTACAACAATACGCCTGACAGGAAAGc                                                                                                                                                                           >  2:76378/1‑250 (MQ=255)                                                                           aaGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTc                                                                                                                                                                     >  1:424368/1‑251 (MQ=255)                                                                            aGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAAt                                                                                                                                                                                                                       >  1:12767/1‑200 (MQ=255)                                                                            aGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAAt                                                                                                                                                                                                                       <  2:12767/200‑1 (MQ=255)                                                                               tCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGt                                                                                                                                                                  <  1:143061/250‑1 (MQ=255)                                                                                ccACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCTATACGCCTGACAGGAAAGCAATGTCAGTa                                                                                                                                                                 <  2:306400/250‑1 (MQ=255)                                                                                ccACACGCAGCGAGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCGGGCGGTGGTATAGGCTGCGGTGTATTCGCGGTTGAAGTTTTCATTGTTCAGCAGGTACAGCAAGTCGCCTGACAGGAAAGCAATGTCAGTAc                                                                                                                                                                <  2:48307/251‑1 (MQ=255)                                                                                   cacGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCgg                                                                                                                                                             >  1:315651/1‑251 (MQ=255)                                                                                         gcgcGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGaa                                                                                                                                                       <  1:360630/251‑1 (MQ=255)                                                                                          cgcgGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAAt                                                                                                                                                      <  1:70894/251‑1 (MQ=255)                                                                                           gcgGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAAt                                                                                                                                                      >  1:210321/1‑250 (MQ=255)                                                                                            gtgATGTTGCAGCGTTGTATCGCGTGTGGCGAAGCCGTTTTCGTCCAGTTCATATGTCCAGGAGGATTTATCGTACTTGCGTTGTTCCGCGTCGTAGCCGGTGAACAGGCCATCTACAGAGCCGTAATCCTCACGCACGATCTGGCTGGCTGTGGGATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGGAGGTGCAGCAGTACGCGTGACAGGAAAGCAATGGCAGTACCGGAACGAATAg                                                                                                                                                    <  1:243307/249‑1 (MQ=255)                                                                                                 gTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGc                                                                                                                                                >  1:129362/1‑250 (MQ=255)                                                                                                  ttGCAGAGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATAAGCCTGACAGGAAAGCAATGACAGTACCGGAACGAATAGGGGCAt                                                                                                                                              >  1:271314/1‑251 (MQ=255)                                                                                                         gTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTc                                                                                                                                       >  1:52126/1‑251 (MQ=255)                                                                                                                         cGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGt                                                                                                                        >  2:162014/1‑250 (MQ=255)                                                                                                                           aaGCCGTTTTCGTCCAGTTCAGAATTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTAcg                                                                                                                     <  2:194192/251‑1 (MQ=255)                                                                                                                              ccGTGTTCGTCCAGTTCAGAAGTCCAGGAGGATTTATCGTACTTGCGTTTTGCCGCGTCGTAGCAGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGGACAGCAATACGCCGGACAGGAAAGCAAGGTCAGTACCGGAACGAAGAGGGGCATAGTGGTCAGCTACCGCCGCCGTACGCGt                                                                                                                  <  2:160720/251‑1 (MQ=255)                                                                                                                                gTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTa                                                                                                                 >  1:159951/1‑250 (MQ=255)                                                                                                                                  tttagtccatgtcagaaGTCCAGGAGGATTTCACGTACTTGCGTTTTCCCGCGGCGTAGCCGGTGAACAGGCCAGTTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTGCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATAAGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATATGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTaaa                                                                                                               <  2:96500/235‑1 (MQ=255)                                                                                                                                      gTCCAGTTCATAAGTCCAGGAGGATTTATCTTTCGTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTAAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTTACAGGAAAGCAATGTCAGTACCGGAACGATTAGGGGCATAGTGGTCAGCTACCGCCGCCGTACGCGTAAAGCGAg                                                                                                          <  2:315651/251‑1 (MQ=255)                                                                                                                                        ccAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGAAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCCCGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGGGTATTCGCGGTTGAATTTTTCCTTGTTCAGCAGGGACAGCAATACGCCTGACCGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGGAGTCAGATACCGCCGCCGTACGCGTAAGGCGAggg                                                                                                        >  2:388207/1‑250 (MQ=255)                                                                                                                                                 aaGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATcac                                                                                                <  1:88980/250‑1 (MQ=255)                                                                                                                                                  aGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGACCTGGCCTTCTTAAAAGCAGTAATCCTCACGCACGATCACGCTGGCGGTGGCATTGGCTTCGGTGTATTCGAGGATGAATTTTTCAt                                                                                                                                                                                                                >  2:191600/1‑137 (MQ=255)                                                                                                                                                  aGTCCAGGAGGAGTTATCGTACTTGCGTTTTTCCGCGTCGTAGCGGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGAGCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCAt                                                                                                                                                                                                                <  1:191600/137‑1 (MQ=255)                                                                                                                                                    tCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCCGGTACAGCAATGCGCCTGACAGGAAAGCAATGTCAGTACCGGCACGAATTGGGGCCTAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATc                                                                                            >  2:155314/1‑251 (MQ=255)                                                                                                                                                      cAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTCGTCAGCTAACGCCGCCGTACGCGTAAAGCGGAGATGGATCACaaac                                                                                            >  2:116407/1‑247 (MQ=255)                                                                                                                                                        ggaggaGTTATCGTACTTGCGTTTTTCCGCGTCGTGGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCGACCGCCGCCTTACGCGTAAAGCGAGGATCGATCACAATCAGCt                                                                                        <  2:211623/251‑1 (MQ=255)                                                                                                                                                             aTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATAAGCTTcgcg                                                                                   >  1:253587/1‑251 (MQ=255)                                                                                                                                                                    gTACTGGCTTTTTTCCGCGGCGTAGTCGTGGAACGGGCCATCTTCAAAGCCTTAATCCCCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTTTATTCTCGGTTGAAGTTGTCATGGTTCAGCAGGTACTGCAATACGCCTTACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATATTAGTCATCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCTCAATCAGCTTCGCGCCGTtgt                                                                            <  2:364746/251‑1 (MQ=255)                                                                                                                                                                              ttttCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGGGGGATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGAGAGGATCGCTCACAATCAGCTTCGCGCCGTTGGGATTTTTGGc                                                                  >  2:298949/1‑251 (MQ=255)                                                                                                                                                                              ttttCCGCGTCGTAGCAAGAACACAGGACATCTTCAAAGCCGTAATCCTCAAGCACGATCAGGCTGGCGGTGGAATAGGATTCGGTGTATTCGCGGTTGCATTTTTCATTGTTCAGCAGGTCCAGCAATACGCCTGGCAGGGACGCAATGTCAGTACCGGAACGCATAGGGGAATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGACCACAATAAGCTTCGCGCCGTTGTGACtttttgc                                                                  >  2:435808/1‑248 (MQ=255)                                                                                                                                                                                 tCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGAGAGGATCGATAACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTc                                                               >  1:264274/1‑251 (MQ=255)                                                                                                                                                                                 cccGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCCCAATCAGTCTTGCGGTGGTATAGGCTTCGGTGTATCCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTc                                                               <  1:376563/250‑1 (MQ=255)                                                                                                                                                                                 cccGCGGCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTACAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCATCTACCGCCGCCGTACGCGTAAAGCGAGGAGCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTc                                                               <  2:129362/250‑1 (MQ=255)                                                                                                                                                                                   cgcgTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTcc                                                              >  1:405970/1‑250 (MQ=255)                                                                                                                                                                                          tAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTAccgcc                                                                                                                             <  1:103057/180‑1 (MQ=255)                                                                                                                                                                                          tAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTAccgcc                                                                                                                             >  2:103057/1‑180 (MQ=255)                                                                                                                                                                                                    aCAGGCCATCTTCAAAGCCGTAATCCTCACGCCCGATCAGGCTGGCGGTGGTATAGGCTTCGGGGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCg                                            <  1:240185/251‑1 (MQ=255)                                                                                                                                                                                                      aggtcgtattCAAAGCCGTAATCCTCACGGACGATTAGGCTGGCGGTGGTATAGGCTGCGGTGTATGCGCGGTTGAATTTTTCATTGTGCAGCTGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTTCCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGTCGGACGCGTAAAGCGAGGATCGATCACAATCATCTTCGAGCCGGTGTGAAGTTTGGCTTCCATCGCCCAGCGGAACCCGAc                                          <  2:274694/243‑1 (MQ=255)                                                                                                                                                                                                                            ccTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGGATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGtt                     <  1:347907/250‑1 (MQ=255)                                                                                                                                                                                                                             cTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGGGTATTCGCGGTTGAACTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGACGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAAACCGACCGGGTGAGCTTCAGCGGCGTTAc                   >  2:63205/1‑251 (MQ=255)                                                                                                                                                                                                                                 cgcacgATCAGGCTAGCGGTGGAATAGGCTTCGTTGAATTCTCGGTTGAATTTTTCATTGTTCCGCAGGGACAACAATACACCTGACAGGGAAGCAATGTCATTACCGGAACGAATAAGGGCATAGTAGTCAGCTACCGCCGCCGACCGCGTAAAGCGCGGATCGATCACAATCAACTTCCCGCCGTTGTGTATTTTGGCATCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTACGCTGCGTTACCGcc               >  2:110561/1‑251 (MQ=255)                                                                                                                                                                                                                                  gcacgATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGccc              >  1:88052/1‑251 (MQ=255)                                                                                                                                                                                                                                     agagcgtgCTGGCGGTGGGATAGGCGTCGGTGTATTCGCGGTTGAATTTTTAATTGTTCAGCAGGTACAGCAATACGCCGGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATATTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATc           <  2:148807/244‑1 (MQ=255)                                                                                                                                                                                                                                              gggCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCGGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCacgacg  <  2:88052/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      GGAACGCGTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACG  >  NC_000913/4084661‑4085148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑