Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,771,979	G→T	intergenic (‑235/‑302)	yibI ← / → mtlA	DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,771,979	0	G	T	100.0%	93.9 / NA	27	intergenic (‑235/‑302)	yibI/mtlA	DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/13);  total (14/13)

TTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCGGCTACCTC  >  NC_000913/3771735‑3772210                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        ttAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGAAGTCAGGCTGATATTTTGACAATAATCCGGGATCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGACGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTca                                                                                                                                                                                                                                    >  2:316484/1‑250 (MQ=255)                ccTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATcaaaac                                                                                                                                                                                                                     >  1:236590/1‑250 (MQ=255)                                           ttttGGGCTGGCATCCGGAAGGAAGTCAGGCTGATATGATGACAATAGTCCGGTTTCGCTATTCACGCCATAACAACAAAGAATAATTTTTAGAGGTGATGAGTTGCTGAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTTAGATCTCAAATATACAACAAACCATGAAATCaaaaagaggacactcctctcagttaaa                                                                                                                                                                                          <  2:193881/250‑23 (MQ=255)                                                           aGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGaa                                                                                                                                                                          >  2:366521/1‑250 (MQ=255)                                                           aGAAGGGAGTCAGGCTGATATTTTAACAATACTCCCGGATCACGCTTCACGCCATAACACCAAAGAATCATTTTTAGAGGTGATGAGATGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATATTGTGATTCCGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGaa                                                                                                                                                                          >  2:436998/1‑250 (MQ=255)                                                              aGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCAGCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGTAAATc                                                                                                                                                                      <  1:345291/251‑1 (MQ=255)                                                                ggAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATcgc                                                                                                                                                                    <  1:202245/251‑1 (MQ=255)                                                                           gATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCGTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCttgttg                                                                                                                                                          <  2:204397/250‑1 (MQ=255)                                                                                                gTTCGCGCTTCTCGCCCTAACACCAAAAACTAATTTTTAGAGGTGATCAGTTGCTTAGTTACATACCGCTTGTATGACGCAGGCATACCATGCTGTAGATAACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCAGACTTGTTGCTTTTTTACACAAGCGTTTtgt                                                                                                                                    >  2:439560/1‑251 (MQ=255)                                                                                                      gATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAAGCGCCGCCGTGTTGCTTTGTTACACAAGCGTTTTGTGATGaa                                                                                                                              <  1:366521/251‑1 (MQ=255)                                                                                                            cGCCATAACACCAAAGAATAATTGTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAacgtcac                                                                                                                        <  2:358196/251‑1 (MQ=255)                                                                                                               cATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAacgtcacgtc                                                                                                                     >  2:164644/1‑251 (MQ=255)                                                                                                                            aataatTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTTGATGACGAATGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTGAAATGCCATTTTTAGCGAAAATCGCCGCCTTTTTGCGTTTTTACACAAGTGTTTTGGGATGAACGTCACGTCAATTGCCTCTCTa                                                                                                        <  2:242986/251‑1 (MQ=255)                                                                                                                                   tttAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACAGTTAAAGGCCATTTTTAGCGAAAAACGCCGCCTTGTGGATTTTTTACACAAGCGTTGTGTGATGAACGTCTCGTCAATGACCTCGCTACCCCCt                                                                                                  <  2:436454/250‑1 (MQ=255)                                                                                                                                    ttAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCta                                                                                                 <  1:316484/250‑1 (MQ=255)                                                                                                                                       gagGTGATGAGTTGCTTAGTTACATAACGATTGGATGACGAAGGCATAACATGCGGTAGATCACATCAGGTGAACGCCGTAAGAAAATAGCTTGTGATTCAGATCACAAATATTCAACAAACGATCAAAACAAAAATGTGACACTACTCACATTGAAAGGCCATTTTTAGCGGAAATCGCGGCCTTGTTGCTTTGTTACACAAGCGTTTTGTGAGGAACGTCGCGTCAAGTACCTCTCTACGCCCTATAtt                                                                                             <  2:419428/251‑1 (MQ=255)                                                                                                                                                                               aaGGCATAACATGCTGTAGATCACATCCGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATAACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGTCCGTCGACTGGAc                                                       >  2:218562/1‑249 (MQ=255)                                                                                                                                                                                     tAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAATAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTTATGAacgtcacg                                                                                                                       <  2:375166/179‑1 (MQ=255)                                                                                                                                                                                     tAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAATAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAacgtcacg                                                                                                                       >  1:375166/1‑179 (MQ=255)                                                                                                                                                                                     tAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAAcc                                               >  2:346046/1‑251 (MQ=255)                                                                                                                                                                                           gCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACAGATTc                                          >  2:378266/1‑250 (MQ=255)                                                                                                                                                                                                  aTCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACACATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACGAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGcc                                    >  1:242297/1‑249 (MQ=255)                                                                                                                                                                                                  aTCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGCCAGTTATCCGATTCAGTGcc                                    >  2:94973/1‑249 (MQ=255)                                                                                                                                                                                                     acaTCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGAtt                               <  1:265480/251‑1 (MQ=255)                                                                                                                                                                                                              gAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACACACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTAt                      >  2:257021/1‑251 (MQ=255)                                                                                                                                                                                                                       aaGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGt             >  2:5334/1‑251 (MQ=255)                                                                                                                                                                                                                                   tgtgATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCGGCTACctc  <  1:312584/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        TTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCGGCTACCTC  >  NC_000913/3771735‑3772210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑