Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,682,515	C→T	M311I (ATG→ATA)	dctA ←	C4 dicarboxylate/orotate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,682,515	0	C	T	100.0%	122.2 / NA	35	M311I (ATG→ATA)	dctA	C4 dicarboxylate/orotate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/15);  total (20/15)

GCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCC‑ACC‑GCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACA  >  NC_000913/3682272‑3682753                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               tccGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATACAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCC‑ACC‑GCAGAGAGCGGCGCCGCCAACACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAA‑ACAGCAACACGATTAACAGCGTGATTTGGTGGCCGATATCCATATGACTGTAAGTGGCCTGGGAGATAAACACCACCGCTATTgcac                                                                                                                                                                                                                                         >  2:254839/2‑248 (MQ=255)    gTTACCGACCAGGTTAGTAAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCC‑ACC‑GCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAA‑GCAACAACACGATTAAAAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTCTTGTCAgg                                                                                                                                                                                                                                       >  2:12208/1‑250 (MQ=255)             cAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCC‑ACC‑GCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGCATATCgctg                                                                                                                                                                                                                             >  2:103632/1‑248 (MQ=255)                        gcgcACGAGCTTCTGACAAAAAGCGGTCGATACGGAGGAACAGCCCCACCCCCTCTACCGGCAAAGGGCCC‑ACC‑GCAGAGAGCGTCGCCGCCAGCACGAAAAAGCCACTCCCCGTTCCCCCTGCCTCCCCTTTAGAAAAAA‑GCAGCAACACGATAAACAGCGTTATTTGGGGGACGATATCCATCGGACTGTTAGGGGCCGGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAgg                                                                                                                                                                                                                  <  1:365479/251‑1 (MQ=255)                                                ggacGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCC‑ACC‑GCAGCGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCACCTTTAGAAGAAA‑GCAGCCACACGATTAACAGCTTGATTTGGTGGACGATATACATCTGACTGTTACTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATAACGATGTGCCATCCAGGTTAAACGAGTAGCATGTCgggga                                                                                                                                                                                           >  2:229037/4‑248 (MQ=255)                                                   cGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCC‑ACC‑GCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGAcc                                                                                                                                                                                       >  1:299889/1‑251 (MQ=255)                                                         cgggggaccagccccacccccgccccGGCAAAAGGCCCCACC‑GCAGTGAGCGCCCCCGCAAGCACGAAAAACCCCCCCCCCATTCCCCCT‑CCCCCCCTTTAAAAAAAAAGCAGCACCACGATAAACAGCGTGATTTGGTGGACGATATCTATCTGACTTTGGGGGGCCGGGGCAAAAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGccc                                                                                                                                                                                  <  2:358178/227‑1 (MQ=255)                                                               ccagcccccgccccGCTACCGGCAAAGGGCCC‑CCC‑GCAGAGAGCGTCCCCGCCAGCACAATAAAGCCCCTACCCGTTACCCCGGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTGGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCTATAACCACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCaccacc                                                                                                                                                                           <  1:42628/240‑1 (MQ=255)                                                                    gcCAGACCCGCTACCGGCAAATGGCCC‑ACC‑GCAGAGAGCGTAGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGAttt                                                                                                                                                                      >  1:416407/1‑251 (MQ=255)                                                                     ccAGACCCGCTACCGGCAAATGGCCC‑ACC‑GCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGTTGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGCCCAGCCCCACCCCCGAtttt                                                                                                                                                                     >  2:89032/1‑250 (MQ=255)                                                                        acccccgcacacgacaaTGGCCC‑CCCCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCCCTCCCCGTTCCCCCTGCCGCCCCTTAAGAAAAAA‑GCAGCAACACGATTAACAGCTTGTTTTGGTGGACGATACCCATCTGCCTGTTAGGGGCCTGGGGGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACg                                                                                                                                                                   <  2:149809/236‑1 (MQ=255)                                                                                            ccccccc‑GCAGAGAGCGTCGCCGCAAGCACGATAAAGCCCCTCCCCGTTCCCCCTGCCGCCCCTTTAAAAAAAA‑GCACCAACACGATTAACAGCGTGATTTGGTGGAAGATATCCATCTTACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCt                                                                                                                                               <  1:395167/246‑1 (MQ=255)                                                                                                  c‑GCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGAGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCg                                                                                                                                          >  1:431646/1‑250 (MQ=255)                                                                                                     cAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGCTTTACGGCAGCCGAGTTTCTCCATCTTGTCgcgc                                                                                                                                       >  2:361933/1‑248 (MQ=255)                                                                                                            cGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATAcgcg                                                                                                                                >  2:151559/1‑251 (MQ=255)                                                                                                                   cccAGCACTATAAACCCCCTCCCTGTTCCCCCCGCCCCCCCTTAAGAAAAAA‑GCAGCAACACGATTACCAGCGTGATTGGGTGGACGATTTCCTTCTGACTGTTAGTGGCCGGGGAGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAgcgc                                                                                                                         <  1:270351/250‑1 (MQ=255)                                                                                                                    ccagccggATAAAGCCACTCCCCGTTCCCCCTGCCCCCCCTTTAGAAAAAA‑GCAGCAACACGATTAACAGCGTGATTTGGGGGACGATATCCATCTGACTGTTAGTGGCCGGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGcc                                                                                                                        <  1:151130/244‑1 (MQ=255)                                                                                                                         acaaTAAAGCCACTCCCCTTTCCCCCTGCCCCCCCTTTAGAAAAAA‑GCAGCAACACGATTAACAGCGTGTTTTGGTGGACAATATCCATCTGCCTTTTAGTGGCCGGGGCGAAAACCACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTc                                                                                                                   <  1:314943/248‑1 (MQ=255)                                                                                                                         aCGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTTGGTGGACGATGTCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTc                                                                                                                   >  1:247005/1‑251 (MQ=255)                                                                                                                           gATAAAGCCACTACCCGTTACCACTGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATGTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCgg                                                                                                                 >  2:75278/1‑251 (MQ=255)                                                                                                                                agcccctccccGTTCCCCCTGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACCGCGGTTTTTGGTGGACGATATCCTTTTGCGTGGTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGAt                                                                                                            <  1:12208/243‑1 (MQ=255)                                                                                                                                    aCTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGa                                                                                                          >  1:271029/1‑249 (MQ=255)                                                                                                                                       ccccGTTCCCCCTCCCGCCCCTTGAGAAGAAA‑GCAGCAACCCGATTAACAGCGTGATTGGGTGGACGATATCCATCGGACTGTTAGGGGCCGGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTc                                                                                                      <  1:69039/249‑1 (MQ=255)                                                                                                                                             tACCCCTGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGCGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGt                                                                                                >  1:166563/1‑250 (MQ=255)                                                                                                                                                              tAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGttcttc                                                                               >  1:238244/1‑250 (MQ=255)                                                                                                                                                                               agcGATGAACAGTGTGATGTGGGTGAAGATATCCATCTGATGGTTAGGGCCCGGGGAAATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATaa                                                              <  2:183081/249‑1 (MQ=255)                                                                                                                                                                                 cGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGGATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAAt                                                            >  1:318447/1‑251 (MQ=255)                                                                                                                                                                                             tGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGACGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGAAAAATTTGAAGATACTg                                                >  2:78479/1‑251 (MQ=255)                                                                                                                                                                                               aTTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGCATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGa                                               >  1:380916/1‑250 (MQ=255)                                                                                                                                                                                                   ggtgcaagattgtcgtctggtttttAGGGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAAcc                                           <  2:247005/227‑1 (MQ=255)                                                                                                                                                                                                      ggACGATATCCATCTGACTGTTAGAGGCCTGGGCGATAAACACCGCCACTATTGTCAGGTATATCGATGTGCCATCACGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCAGACTCGGAAGATGAAGTCCCCCGTAAAATCAGCAGCTCTTCAAGGATTTAGCGGATAATGTTGAAGATACTGAAACCTGTc                                       >  2:18558/1‑251 (MQ=255)                                                                                                                                                                                                                         gTTAGGGGCCGGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAAccc                     <  1:361933/250‑1 (MQ=255)                                                                                                                                                                                                                          ttAGTAGCCTGGGCGATAATCACCGACGCTATTGTCAGGTCTATCGTTTTGCCCTCAAGGATAAAAGAGAAGACTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGATTCTCCATCTTGTCGCGCATACGCGGCAACGCAGACTCGGAAGATGAAATCCCCAGTACACTAAGCAGTTCATCACGGATGTAGCGGATAAATTTGCAGATACTGAAAACAGTAGCTTTCGCGTTTGAAcaca                    >  2:372812/1‑247 (MQ=255)                                                                                                                                                                                                                                gccggggCGATAAACCCCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAg             <  1:227109/247‑1 (MQ=255)                                                                                                                                                                                                                                           aaaCACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACa  <  1:256807/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               GCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCC‑ACC‑GCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAA‑GCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACA  >  NC_000913/3682272‑3682753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑