Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,474,425	T→C	K43R (AAA→AGA)	rpsL ←	30S ribosomal subunit protein S12

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,474,425	0	T	C	100.0%	235.1 / NA	66	K43R (AAA→AGA)	rpsL	30S ribosomal subunit protein S12
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/33);  total (33/33)

CCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATAC  >  NC_000913/3474183‑3474664                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 ccTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCAAACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAg                                                                                                                                                                                                                                            >  2:227097/1‑249 (MQ=255)     aGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGgt                                                                                                                                                                                                                                        >  1:293162/1‑249 (MQ=255)         cccTTCCCGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCGGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACTCCCGGG‑GGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGATTACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGtat                                                                                                                                                                                                                                   <  2:168295/248‑1 (MQ=255)                cGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGACGGCGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGACACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGCGTGGTAGTATATACACg                                                                                                                                                                                                                            >  2:159613/1‑250 (MQ=255)                          ggAACGAGCCTGCTTACGGCCTTAAACGCCGGACCAGTCAAGCGCCCCCCGTACGGTGTGGTA‑CCAACCCCCGGGGAGGCCTTTACCCCGACCGCCACGGATCAGGATCCCGGAGTGCTCCTGCAGGTTGTGCCCTTCACCCCCGATGTAGGAAGTCCCTTCGAACCCGTTAGTCGGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGTTTTTCTAG‑AGTGGTAGTATATACACGAGTACA‑TACGcc                                                                                                                                                                                                               <  1:232259/251‑1 (MQ=255)                           gAACGAGCCTGCTTACGGTCTTGAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTACCGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGcc                                                                                                                                                                                                               <  2:100956/251‑1 (MQ=255)                             acgagctggCTTTCGGTATTTAACGCCGGAGCAGTCAAGCGCCCCACGTACGGTGTGGTAACGACCACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGTAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCAc                                                                                                                                                                                                             <  1:406188/243‑1 (MQ=255)                              cGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACg                                                                                                                                                                                                            <  1:59389/251‑1 (MQ=255)                               gAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGt                                                                                                                                                                                                           >  1:362736/1‑251 (MQ=255)                                  ccTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCCCCACGGACGGGGTGGTAACGAACACCCGGG‑AGGTCTTAAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCTCCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTGTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGtttt                                                                                                                                                                                                        <  2:373250/251‑1 (MQ=255)                                       ttACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCgg                                                                                                                                                                                                   >  1:186238/1‑251 (MQ=255)                                        tACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCCCGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCg                                                                                                                                                                                                    <  1:296030/249‑1 (MQ=255)                                           ggTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCAt                                                                                                                                                                                               >  1:290562/1‑251 (MQ=255)                                               tttAACGCCGGAGCAGTCAAGCGCAGCACGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGCTCACGGAGTGCTCCTGCAGGTTGTGACCTTCACAACCGCTGTAGGAAGTCACTTCGAAACAGTTAGTCAGACGACCAAGGCATACTTTACGCAGCGCGGAGGTAGGTTTTCTAGGCGTGGTAGTATATACACGAG‑ACACTACGCCACGTTTTTGCGGGCATGCtt                                                                                                                                                                                           >  2:301687/1‑251 (MQ=255)                                                      ccGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAgcgc                                                                                                                                                                                    >  1:35326/1‑251 (MQ=255)                                                         ggtgaggcAAGCGCACCACTTCCGGTGTGGTAACGGACACCCGGG‑AGGTCGTTAACACTACCGCCACGGATCAGGATCACGGAGTGCTCTTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGGGCTGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTACCAGCGCAgg                                                                                                                                                                                 <  2:264507/244‑1 (MQ=255)                                                          aGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGc                                                                                                                                                                                >  1:331583/1‑251 (MQ=255)                                                            cAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCAc                                                                                                                                                                              >  1:350147/1‑251 (MQ=255)                                                             aGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACg                                                                                                                                                                             >  1:377473/1‑251 (MQ=255)                                                             aGTCAACCGCACCCAGGACGGTGGGGGAACGAACACCCGGG‑CGGCCTTTACCACGCCCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TCCGCCACGTTTTTGCGTGCATGCTTCCATCGCATGCACg                                                                                                                                                                             <  2:49601/251‑1 (MQ=255)                                                                  atcgaccctgttgcGGTGTGGTAACGACCACCCGGG‑AGGTTTTTAACACGACCGCCACGGATCATGATCACGTAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑GACGCCACGTTTTTGCGGGCATGCTTCCTGCGGAGGCACGTTGCt                                                                                                                                                                        <  2:409756/238‑1 (MQ=255)                                                                          acGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCa                                                                                                                                                                 >  2:408949/1‑250 (MQ=255)                                                                            gtgcGGTGTGGAAACTAACACCCGGG‑AGTTTTTTAACCCGGCCTCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGAGTTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTTCA‑TACGCCACGTTTTTGCGTGCATGCTTCCAGCGCAGGCCCTTTGCTGTTTGCAACt                                                                                                                                                              <  2:282008/248‑1 (MQ=255)                                                                                     ggTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGc                                                                                                                                                      >  1:284813/1‑250 (MQ=255)                                                                                      gTAACGAACGCCCGGT‑AGGTCTTGAACACGACCGCCACGTATCAGGATCTCGGTGTGCGCCTGCAGGTTGTGATCTTCACCACCGATGTATGAAGTCGCTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGGGTTCGGTTTTCTAGTAGTGTTAGTATATACACGAGGACG‑TACGTCATGTTTTGGCGGGCATGCTTCCAGCGCAGTGGCGTTGGTTTTTGCAGCTGTGCGAGCAc                                                                                                                                                    <  2:209565/251‑1 (MQ=255)                                                                                        aaCGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGACACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGCGCACGt                                                                                                                                                  >  2:136989/1‑251 (MQ=255)                                                                                          cGAACACCGGGG‑AGGTATTAAAAACGACCGCCACGGAACAGGATCACGGAGTGCTCCTTCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGTTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTgg                                                                                                                                                <  2:383603/251‑1 (MQ=255)                                                                                               ccccGGG‑ATGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCAAACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGc                                                                                                                                           <  1:227097/250‑1 (MQ=255)                                                                                                  cGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGCAGTCACTTCGAAACCGTTAGTCAGACGAACACGGAATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCa                                                                                                                                                                                        >  1:22878/1‑203 (MQ=255)                                                                                                    gg‑AGGTCTTTAACCCAACCGCAACGGATCAGGATCCCGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGCAGTCACTTCGAAACAGTTAGTCAGCCGACCACGGCCTACTTTACGCAGCGCGGAGTTCGGTTTTCTCGGAGGGGTAGTATATCCACGAGTACA‑TACGCAACGTTTTTGCGGGAATGCTTCCAGCGCAGGCCCGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGAGTAcc                                                                                                                                      >  2:50027/1‑251 (MQ=255)                                                                                                            tttAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGt                                                                                                                               >  1:378570/1‑251 (MQ=255)                                                                                                                 cacGAACGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACt                                                                                                                          >  1:133021/1‑251 (MQ=255)                                                                                                                        gCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGcc                                                                                                                    >  1:231866/1‑250 (MQ=255)                                                                                                                        gCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCa                                                                                                                   <  1:212774/251‑1 (MQ=255)                                                                                                                             ggatcaggatcaCGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTGTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTGTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTGAACTGTTGCCATTaaa                                                                                                              <  2:211339/251‑1 (MQ=255)                                                                                                                                         cGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGtt                                                                                                  >  1:85571/1‑251 (MQ=255)                                                                                                                                          ggAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGttt                                                                                                 <  1:338620/251‑1 (MQ=255)                                                                                                                                          ggAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGACACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGttt                                                                                                 >  1:94398/1‑251 (MQ=255)                                                                                                                                          ggAGTGCTCCGGCAGGTTGTGTCCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTTCGGTCATGCGTCCAGCGCAGGCACGTTGCTTGTCGCAACTTTGCGAGCACGTGGTGTGCGTACCATCTGGTTAACTGTTGCCATTAAATAGCGCCTGGttt                                                                                                 <  1:175143/251‑1 (MQ=255)                                                                                                                                           gAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGtttt                                                                                                <  1:191560/251‑1 (MQ=255)                                                                                                                                               gCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGc                                                                                             <  1:330675/250‑1 (MQ=255)                                                                                                                                                  ccTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCtttt                                                                                         <  1:111142/251‑1 (MQ=255)                                                                                                                                                        ggTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCg                                                                                   >  2:35177/1‑251 (MQ=255)                                                                                                                                                            gtgACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTaa                                                                                <  1:301687/250‑1 (MQ=255)                                                                                                                                                            gtgACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATAATTTACGCAGCGCTGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTATCTGTTGCCATTACATAGCGCCTGGTTTTAGCTTTTGCTTCGTaa                                                                                <  1:439625/250‑1 (MQ=255)                                                                                                                                                               aCCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAacac                                                                            <  2:429758/251‑1 (MQ=255)                                                                                                                                                                  ttCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCCGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTACCTAGCTCCTGGTTTTAGCTTTTGCTTCGTAACCACGt                                                                          >  2:132114/1‑250 (MQ=255)                                                                                                                                                                     accaccGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTAGATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTGCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGtaata                                                                      <  1:440279/251‑1 (MQ=255)                                                                                                                                                                        cccGATGTAGGAAGTCACTGCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGATTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACGGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTGCGTAAACTCGTAATaaaa                                                                   <  2:186238/250‑1 (MQ=255)                                                                                                                                                                              gTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCt                                                             >  2:296668/1‑251 (MQ=255)                                                                                                                                                                                aGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTc                                                            >  2:154695/1‑250 (MQ=255)                                                                                                                                                                                  gAAGTCACTTCGAAACCGGTAGTCAGACGAACACGGCATACTTTACGCATCGCGGAGTTCTGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCAGTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTATACACGTAATAAAACGTCCTcaca                                                         <  2:77238/251‑1 (MQ=255)                                                                                                                                                                                     gTCACTTCGACACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGTCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAACCGTCCTaacaca                                                       >  2:127/1‑250 (MQ=255)                                                                                                                                                                                         cTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATg                                                  >  1:24392/1‑251 (MQ=255)                                                                                                                                                                                                         cAGACGAACACGGCATAGTTTACGCAGCGAGGAGTTCGGTTTTCTAGGAGTGGCAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCCACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTATATAGCTCCTGGTTTTAGATTTTGCTTCGTAAACACGTAATAAAACGTACTCACACAATATGAGGACGCCGAAtttt                                   >  2:51052/1‑250 (MQ=255)                                                                                                                                                                                                         cAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGACtttt                                   >  1:88715/1‑250 (MQ=255)                                                                                                                                                                                                           gACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAgg                                >  1:328223/1‑251 (MQ=255)                                                                                                                                                                                                             cGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACTCGTAATAAAACGACCTCACACAATATGAGGACGCCGAATTTTAGGGc                              <  2:176355/251‑1 (MQ=255)                                                                                                                                                                                                               aaCAAGGCATTCGTTACGCAGCGCGGAGTCCGGTTTTCTAGGAGTGGTAGTATATACACGTGTACA‑GTCGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCGTTTGCTTCGTAAACACGTAATAAAACGTCCTCACCCAATATGAGGACGCCGAATTTTATGGCGa                            <  2:15855/251‑1 (MQ=255)                                                                                                                                                                                                                 cacGGCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATg                          <  1:254943/251‑1 (MQ=255)                                                                                                                                                                                                                    ggCATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCg                       <  2:290562/251‑1 (MQ=255)                                                                                                                                                                                                                      cATACTTTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGa                      >  1:237530/1‑250 (MQ=255)                                                                                                                                                                                                                      cATACTGTACGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAACTTTAGGGCGATTCCGaa                     <  2:103492/251‑1 (MQ=255)                                                                                                                                                                                                                              aCGCAGCGCGGAGTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTc             <  1:95174/251‑1 (MQ=255)                                                                                                                                                                                                                                        gAGTTCGGTTTTCTAGGAGAGGTAGTATATACACGCGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGAACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTAAGGGCGATGCCGAACAGGAGTCAAGAAatat    >  2:170239/1‑250 (MQ=255)                                                                                                                                                                                                                                          gTTCGGTTTTCTAGGAGTGGTAGTATATACACGAGTACA‑TAAGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCCTTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACCATATGAGGACGCAGAATTTTAGGGCGATGCCGAAACGGTGTCAAGAAATATAc  >  2:249618/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 CCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGG‑AGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACA‑TACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATAC  >  NC_000913/3474183‑3474664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑